1887

Abstract

A novel species, gen. nov., sp. nov., was isolated from the rhizosphere, roots and stems of salt-tolerant, mangrove-associated wild rice ( Tateoka) using nitrogen-free, semi-solid LGI medium at pH 5·5. Strains were Gram-negative, rod-shaped and motile with peritrichous flagella. The strains grew well in the presence of 0·35 % acetic acid, 3 % NaCl and 1 % KNO, and produced acid from -arabinose, -glucose, glycerol, ethanol, -mannose, -galactose and sorbitol. They oxidized ethanol and grew well on mannitol and glutamate agar. The fatty acids 18 : 17/9/12 and 19 : 0cyclo 8 constituted 30·41 and 11·80 % total fatty acids, respectively, whereas 13 : 1 AT 12–13 was found at 0·53 %. DNA G+C content was 57·6–59·9 mol% and the major quinone was Q-10. Phylogenetic analysis based on 16S rRNA gene sequences showed that these strains were related to the genera , , , , and in the . Isolates were able to fix nitrogen and solubilized phosphate in the presence of NaCl. Based on overall analysis of the tests and comparison with the characteristics of members of the , a novel genus and species is proposed for these isolates, gen. nov., sp. nov. The type strain is PA51 (=LMG 21291=MTCC 3852).

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2004-07-01
2020-12-03
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