1887

Abstract

sp. nov. is proposed for two moderately halophilic, psychrophilic, alkalitolerant bacteria, LC1 (=DSM 15516=ATCC BAA-759) and LC2 (=DSM 15517=ATCC BAA-760), both of which were isolated from a soil sample around the lake Laguna Colorada, located at 4300 m above sea level in the south-west region of Bolivia. The bacteria are aerobic, motile, Gram-negative rods that produce colonies with a cream pigment. Moreover, they are heterotrophs that are able to utilize various carbohydrates as carbon sources. The organisms reduce nitrate and show tryptophan deaminase activity. The genomic DNA G+C contents were 51·4 mol% for isolate LC1 and 52·6 mol% for isolate LC2. Based on 16S rDNA sequence analysis, isolates LC1 and LC2 were identified as members of the genus and clustered closely with DSM 3051 and DSM 5425. However, DNA–DNA relatedness between the new isolates and the closest related species was low.

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2004-05-01
2019-10-15
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References

  1. Arahal, D. R., Ludwig, W., Schleifer, K. H. & Ventosa, A. ( 2002; ). Phylogeny of the family Halomonadaceae based on 23S and 16S rDNA sequence analyses. Int J Syst Evol Microbiol 52, 241–249.
    [Google Scholar]
  2. Baumgarte, S., Moore, E. R. B. & Tindall, B. J. ( 2001; ). Re-examining the 16S rDNA sequence of Halomonas salina. Int J Syst Evol Microbiol 51, 51–53.
    [Google Scholar]
  3. Fendrich, C. ( 1988; ). Halovibrio variabilis gen. nov. sp. nov, Pseudomonas halophila sp. nov. and a new halophilic aerobic coccoid Eubacterium from Great Salt Lake, Utah, USA. Syst Appl Microbiol 11, 36–43.[CrossRef]
    [Google Scholar]
  4. Hurlbert, S. H. & Chang, C. C. Y. ( 1988; ). The distribution, structure, and composition of freshwater ice deposits in Bolivian salt lakes. Hydrobiologia 158, 271–299.[CrossRef]
    [Google Scholar]
  5. Johnson, J. L. ( 1994; ). Similarity analysis of DNAs. In Methods for General and Molecular Bacteriology, pp. 655–682. Edited by P. Gerhardt, R. G. E. Murray, W. A. Wood & N. R. Krieg. Washington, DC: American Society for Microbiology.
  6. Jones, T. A. (editor) ( 1993; ). A Directory of Wetlands of International Importance, 4th edn, vol. 4. Gland, Switzerland: Ramsar Convention Bureau.
  7. Kumar, S., Tamura, K., Jakobsen, I. B. & Nei, M. ( 2001; ). mega2: molecular evolutionary genetic analysis software. Bioinformatics 17, 1244–1245.[CrossRef]
    [Google Scholar]
  8. Logan, N. A. & Berkeley, C. W. ( 1984; ). Identification of Bacillus strains using the API system. J Gen Microbiol 130, 1871–1882.
    [Google Scholar]
  9. Maidak, B. L., Cole, J. R., Lilburn, T. G. & 9 other authors ( 2000; ). The RDP (Ribosomal Database Project) continues. Nucleic Acids Res 28, 173–174.[CrossRef]
    [Google Scholar]
  10. Marmur, J. ( 1961; ). A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3, 208–218.[CrossRef]
    [Google Scholar]
  11. Morita, R. Y. ( 1975; ). Psychrophilic bacteria. Bacteriol Rev 39, 144–167.
    [Google Scholar]
  12. Oren, A. ( 2002; ). Diversity of halophilic microorganisms: environments, phylogeny, physiology, and applications. J Ind Microbiol Biotechnol 28, 56–63.[CrossRef]
    [Google Scholar]
  13. Reddy, G. S. N., Raghavan, P. U. M., Sarita, N. B., Prakash, J. S. S., Nagesh, N., Delille, D. & Shivaji, S. ( 2003; ). Halomonas glaciei sp. nov. isolated from fast ice of Adelie Land, Antartica. Extremophiles 7, 55–61.
    [Google Scholar]
  14. Risacher, F. ( 1992; ). Géochimie des lacs salés et croûtes de sel de l'Altiplano Bolivien. Sci Géol Bull 45, 135–214 (in French).
    [Google Scholar]
  15. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  16. Smibert, R. M. & Krieg, N. R. ( 1994; ). Phenotypic characterization. In Methods for General and Molecular Bacteriology, pp. 607–655. Edited by P. Gerhardt, R. G. E. Murray, W. A. Wood & N. R. Krieg. Washington, DC: American Society for Microbiology.
  17. Tindall, B. J. ( 1994; ). Chemical analysis of Archaea and Bacteria: a critical evaluation of its use in taxonomy and identification. In Bacterial Diversity and Systematics (FEMS Symposium no. 75), pp. 243–258. Edited by F. G. Priest, A. Ramos-Cormenzana & B. J. Tindall. New York: Plenum.
  18. Ventosa, A., Quesada, E., Rodriguez-Valera, F., Ruiz-Berraquero, F. & Ramos-Cormenzana, A. ( 1982; ). Numerical taxonomy of moderately halophilic Gram-negative rods. J Gen Microbiol 128, 1959–1968.
    [Google Scholar]
  19. Ventosa, A., Nieto, J. J. & Oren, A. ( 1998; ). Biology of moderately halophilic aerobic bacteria. Microbiol Mol Biol Rev 62, 504–544.
    [Google Scholar]
  20. Vreeland, R. H., Litchfield, C. D., Martin, E. L. & Elliot, E. ( 1980; ). Halomonas elongata, a new genus and species of extremely salt-tolerant bacteria. Int J Syst Bacteriol 30, 485–495.[CrossRef]
    [Google Scholar]
  21. Wayne, L. G., Brenner, D. J., Colwell, R. R. & 9 other authors ( 1987; ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef]
    [Google Scholar]
  22. Weisburg, W. G., Barns, S. M., Pelletier, D. A. & Lane, D. J. ( 1991; ). 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol 173, 697–703.
    [Google Scholar]
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vol. , part 3, pp. 721 – 725

Comparison of differential characteristics of isolates LC1 and LC2 with some type strains of the genus .

Transmission electron micrographs of uranyl acetate-stained cells of isolates LC1 and LC2.

Scanning electron micrographs of LC1 .

Scanning electron micrographs of LC2.

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