@article{mbs:/content/journal/ijsem/10.1099/ijs.0.02800-0, author = "Quillaguamán, Jorge and Hatti-Kaul, Rajni and Mattiasson, Bo and Alvarez, Maria Teresa and Delgado, Osvaldo", title = "Halomonas boliviensis sp. nov., an alkalitolerant, moderate halophile isolated from soil around a Bolivian hypersaline lake", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2004", volume = "54", number = "3", pages = "721-725", doi = "https://doi.org/10.1099/ijs.0.02800-0", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.02800-0", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", abstract = " Halomonas boliviensis sp. nov. is proposed for two moderately halophilic, psychrophilic, alkalitolerant bacteria, LC1T (=DSM 15516T=ATCC BAA-759T) and LC2 (=DSM 15517=ATCC BAA-760), both of which were isolated from a soil sample around the lake Laguna Colorada, located at 4300 m above sea level in the south-west region of Bolivia. The bacteria are aerobic, motile, Gram-negative rods that produce colonies with a cream pigment. Moreover, they are heterotrophs that are able to utilize various carbohydrates as carbon sources. The organisms reduce nitrate and show tryptophan deaminase activity. The genomic DNA G+C contents were 51·4 mol% for isolate LC1T and 52·6 mol% for isolate LC2. Based on 16S rDNA sequence analysis, isolates LC1T and LC2 were identified as members of the genus Halomonas and clustered closely with Halomonas variabilis DSM 3051T and Halomonas meridiana DSM 5425T. However, DNA–DNA relatedness between the new isolates and the closest related Halomonas species was low.", }