1887

Abstract

A novel marine actinomycete, designated CXB654, was isolated from marine sediment collected at a depth of 17.5 m near the Yellow Sea Cold Water Mass, China. Optimal growth occurred at 37.0 °C, at pH 7.0–8.0 and in 3–8 % (w/v) NaCl. Strain CXB654 formed branched substrate mycelium without fragmentation. Abundant aerial mycelium differentiated into long or short chains of spores and spores were elliptical and cylindrical with spiny surfaces. Strain CXB654 contained -diaminopimelic acid as the diagnostic diamino acid, and ribose and glucose as the major whole-cell components. Phospholipids were diphosphatidylglycerol, phosphatidylcholine, phosphatidylglycerol and phosphatidylinositol. MK-10(H), MK-10(H) and MK-9(H) were the predominant menaquinones. The major fatty acids were i-C (24.46 %), ai-C (20.66 %) and C (20.14 %). The DNA G+C content was 71.1 mol%. Comparative analysis of 16S rRNA gene sequences showed that the novel strain was most closely related to genera within the family , but formed a separate lineage. Highest sequence similarities were to DSM 45312 (96.6 %), YIM 90462 (96.5 %) and DSM 45154 (96.1 %). On the basis of phenotypic, chemotaxonomic and phylogenetic distinctiveness, strain CXB654 was considered to represent a novel species in a new genus in the family , and the name gen. nov., sp. nov. is proposed; the type strain is CXB654 ( = DSM 45414 = LMG 25485).

Funding
This study was supported by the:
  • National High Technology Research and Development Program of China (Award 2007AA09Z434)
  • National Natural Science Foundation of China (Award 40876067)
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2011-12-01
2024-12-05
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References

  1. Cai M., Zhi X.-Y., Tang S.-K., Zhang Y.-Q., Xu L.-H., Li W.-J. 2008; Streptomonospora halophila sp. nov., a halophilic actinomycete isolated from a hypersaline soil. Int J Syst Evol Microbiol 58:1556–1560 [View Article][PubMed]
    [Google Scholar]
  2. Chen Y.-G., Cui X.-L., Kroppenstedt R. M., Stackebrandt E., Wen M.-L., Xu L.-H., Jiang C.-L. 2008; Nocardiopsis quinghaiensis sp. nov., isolated from saline soil in China. Int J Syst Evol Microbiol 58:699–705 [View Article][PubMed]
    [Google Scholar]
  3. Chen Y.-G., Wang Y.-X., Zhang Y.-Q., Tang S.-K., Liu Z.-X., Xiao H.-D., Xu L.-H., Cui X.-L., Li W.-J. 2009; Nocardiopsis litoralis sp. nov., a halophilic marine actinomycete isolated from a sea anemone. Int J Syst Evol Microbiol 59:2708–2713 [View Article][PubMed]
    [Google Scholar]
  4. Chun J., Lee J.-H., Jung Y., Kim M., Kim S., Kim B. K., Lim Y.-W. 2007; EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57:2259–2261 [View Article][PubMed]
    [Google Scholar]
  5. Cui X. L., Mao P. H., Zeng M., Li W. J., Zhang L. P., Xu L. H., Jiang C. L. 2001; Streptimonospora salina gen. nov., sp. nov., a new member of the family Nocardiopsaceae . Int J Syst Evol Microbiol 51:357–363[PubMed]
    [Google Scholar]
  6. Gonzalez C., Gutierrez C., Ramirez C. 1978; Halobacterium vallismortis sp. nov. An amylolytic and carbohydrate-metabolizing, extremely halophilic bacterium. Can J Microbiol 24:710–715 [View Article][PubMed]
    [Google Scholar]
  7. Groth I., Rodríguez C., Schütze B., Schmitz P., Leistner E., Goodfellow M. 2004; Five novel Kitasatospora species from soil: Kitasatospora arboriphila sp. nov., K. gansuensis sp. nov., K. nipponensis sp. nov., K. paranensis sp. nov. and K. terrestris sp. nov.. Int J Syst Evol Microbiol 54:2121–2129 [View Article][PubMed]
    [Google Scholar]
  8. Hall T. A. 1999; BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41:95–98
    [Google Scholar]
  9. Kämpfer P., Schäfer J., Lodders N., Martin K. 2010; Murinocardiopsis flavida gen. nov., sp. nov., an actinomycete isolated from indoor walls. Int J Syst Evol Microbiol 60:1729–1734 [View Article][PubMed]
    [Google Scholar]
  10. Kelly K. L. 1964 Inter-Society Color Council – National Bureau of Standards Color Name Charts Illustrated with Centroid Colors Washington, DC: US Government Printing Office;
    [Google Scholar]
  11. Kroppenstedt R. M., Evtushenko L. I. 2006; The family Nocardiopsaceae . In The Prokaryotes: A Handbook on the Biology of Bacteria, 3rd edn. vol. 3 pp. 754–795 Edited by Dworkin M., Falkow S., Rosenberg E., Schleifer K. H., Stackebrandt E. New York: Springer-Verlag;
    [Google Scholar]
  12. Li M.-G., Li W.-J., Xu P., Cui X.-L., Xu L.-H., Jiang C.-L. 2003; Nocardiopsis xinjiangensis sp. nov., a halophilic actinomycete isolated from a saline soil sample in China. Int J Syst Evol Microbiol 53:317–321 [View Article][PubMed]
    [Google Scholar]
  13. Li W.-J., Park D.-J., Tang S.-K., Wang D., Lee J.-C., Xu L.-H., Kim C.-J., Jiang C.-L. 2004; Nocardiopsis salina sp. nov., a novel halophilic actinomycete isolated from saline soil in China. Int J Syst Evol Microbiol 54:1805–1809 [View Article][PubMed]
    [Google Scholar]
  14. Li W.-J., Kroppenstedt R. M., Wang D., Tang S.-K., Lee J.-C., Park D.-J., Kim C.-J., Xu L.-H., Jiang C.-L. 2006; Five novel species of the genus Nocardiopsis isolated from hypersaline soils and emended description of Nocardiopsis salina Li et al. 2004. Int J Syst Evol Microbiol 56:1089–1096 [View Article][PubMed]
    [Google Scholar]
  15. Li W.-J., Xu P., Schumann P., Zhang Y. Q., Pukall R., Xu L. H., Stackebrandt E., Jiang C. L. 2007; Georgenia ruanii sp. nov., a novel actinobacterium isolated from forest soil in Yunnan (China), and emended description of the genus Georgenia . Int J Syst Evol Microbiol 57:1424–1428 [View Article][PubMed]
    [Google Scholar]
  16. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [View Article]
    [Google Scholar]
  17. Meyer J. 1976; Nocardiopsis, a new genus of the order Actinomycetales . Int J Syst Bacteriol 26:487–493 [View Article]
    [Google Scholar]
  18. Rainey F. A., Ward-Rainey N., Kroppenstedt R. M., Stackebrandt E. 1996; The genus Nocardiopsis represents a phylogenetically coherent taxon and a distinct actinomycete lineage: proposal of Nocardiopsaceae fam. nov.. Int J Syst Bacteriol 46:1088–1092 [View Article][PubMed]
    [Google Scholar]
  19. Sabry S. A., Ghanem N. B., Abu-Ella G. A., Schumann P., Stackebrandt E., Kroppenstedt R. M. 2004; Nocardiopsis aegyptia sp. nov., isolated from marine sediment. Int J Syst Evol Microbiol 54:453–456 [View Article][PubMed]
    [Google Scholar]
  20. Shirling E. B., Gottlieb D. 1966; Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16:313–340 [View Article]
    [Google Scholar]
  21. Tamura K., Dudley J., Nei M., Kumar S. 2007; mega4: Molecular Evolutionary Genetics Analysis (mega) software version 4.0. Mol Biol Evol 24:1596–1599 [View Article][PubMed]
    [Google Scholar]
  22. Tang S.-K., Tian X.-P., Zhi X.-Y., Cai M., Wu J.-Y., Yang L.-L., Xu L.-H., Li W.-J. 2008; Haloactinospora alba gen. nov., sp. nov., a halophilic filamentous actinomycete of the family Nocardiopsaceae . Int J Syst Evol Microbiol 58:2075–2080 [View Article][PubMed]
    [Google Scholar]
  23. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [View Article][PubMed]
    [Google Scholar]
  24. Tian X.-P., Tang S.-K., Dong J.-D., Zhang Y.-Q., Xu L.-H., Zhang S., Li W.-J. 2009; Marinactinospora thermotolerans gen. nov., sp. nov., a marine actinomycete isolated from a sediment in the northern South China Sea. Int J Syst Evol Microbiol 59:948–952 [View Article][PubMed]
    [Google Scholar]
  25. Waksman S. A. 1961 The Actinomycetes, Classification, Identification and Description of Genera and Species vol. 2 Baltimore: Williams & Wilkins;
    [Google Scholar]
  26. Xu P., Li W.-J., Tang S.-K., Zhang Y.-Q., Chen G.-Z., Chen H.-H., Xu L.-H., Jiang C.-L. 2005; Naxibacter alkalitolerans gen. nov., sp. nov., a novel member of the family ‘Oxalobacteraceae’ isolated from China. Int J Syst Evol Microbiol 55:1149–1153 [View Article][PubMed]
    [Google Scholar]
  27. Yang L.-L., Tang S.-K., Zhang Y.-Q., Zhi X.-Y., Wang D., Xu L.-H., Li W.-J. 2008a; Thermobifida halotolerans sp. nov., isolated from a salt mine sample, and emended description of the genus Thermobifida . Int J Syst Evol Microbiol 58:1821–1825 [View Article][PubMed]
    [Google Scholar]
  28. Yang R.-L., Zhang L.-P., Guo L.-G., Shi N., Lu Z.-T., Zhang X.-M. 2008b; Nocardiopsis valliformis sp. nov., an alkaliphilic actinomycete isolated from alkali lake soil in China. Int J Syst Evol Microbiol 58:1542–1546 [View Article][PubMed]
    [Google Scholar]
  29. Zhang Z., Wang Y., Ruan J. 1998; Reclassification of Thermomonospora and Microtetraspora . Int J Syst Bacteriol 48:411–422[PubMed] [CrossRef]
    [Google Scholar]
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