@article{mbs:/content/journal/ijsem/10.1099/ijs.0.027383-0, author = "Chang, Xianbo and Liu, Wenzheng and Zhang, Xiao-Hua", title = "Spinactinospora alkalitolerans gen. nov., sp. nov., an actinomycete isolated from marine sediment", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2011", volume = "61", number = "12", pages = "2805-2810", doi = "https://doi.org/10.1099/ijs.0.027383-0", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.027383-0", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", abstract = "A novel marine actinomycete, designated CXB654T, was isolated from marine sediment collected at a depth of 17.5 m near the Yellow Sea Cold Water Mass, China. Optimal growth occurred at 37.0 °C, at pH 7.0–8.0 and in 3–8 % (w/v) NaCl. Strain CXB654T formed branched substrate mycelium without fragmentation. Abundant aerial mycelium differentiated into long or short chains of spores and spores were elliptical and cylindrical with spiny surfaces. Strain CXB654T contained meso-diaminopimelic acid as the diagnostic diamino acid, and ribose and glucose as the major whole-cell components. Phospholipids were diphosphatidylglycerol, phosphatidylcholine, phosphatidylglycerol and phosphatidylinositol. MK-10(H8), MK-10(H6) and MK-9(H8) were the predominant menaquinones. The major fatty acids were i-C16 : 0 (24.46 %), ai-C17 : 0 (20.66 %) and C18 : 0 (20.14 %). The DNA G+C content was 71.1 mol%. Comparative analysis of 16S rRNA gene sequences showed that the novel strain was most closely related to genera within the family Nocardiopsaceae, but formed a separate lineage. Highest sequence similarities were to Murinocardiopsis flavida DSM 45312T (96.6 %), Thermobifida halotolerans YIM 90462T (96.5 %) and Marinactinospora thermotolerans DSM 45154T (96.1 %). On the basis of phenotypic, chemotaxonomic and phylogenetic distinctiveness, strain CXB654T was considered to represent a novel species in a new genus in the family Nocardiopsaceae, and the name Spinactinospora alkalitolerans gen. nov., sp. nov. is proposed; the type strain is CXB654T ( = DSM 45414T = LMG 25485T).", }