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Abstract

A Gram-stain-negative, aerobic, rod-shaped bacterium, designated strain J3-A127, was isolated from the roots of fresh rice plants (). Cells were non-motile and no flagellum was detected. Comparison of 16S rRNA gene sequences indicated that the strain was phylogenetically related to species of the genus , with closest similarity to Alt 505 (96.4 %). The low levels of 16S rRNA gene sequence similarity (<90 %) found between the , , and gene sequences of strain J3-A127 and the type strains of recognized species of the genus also indicated that it represented a separate species. The temperature range for growth was 10–40 °C (optimum around 28 °C) and the pH range was 6.0–11.0 (optimum pH 7.0–8.0). Strain J3-A127 tolerated NaCl concentrations up to 5.0 % (w/v). The strain was catalase- and oxidase-positive. The main cellular fatty acids were summed feature 8 (Cω7 and/or Cω6; 46.7 %). The DNA G+C content of strain J3-A127 was 59.5 mol%. Strain J3-A127 did not form any nodules on four different legumes and the and genes were not detected by PCR. According to physiological and biochemical characteristics and genotypic data, strain J3-A127 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is J3-A127 ( = ACCC 10380 = KCTC 23294).

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2011-10-01
2020-01-22
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vol. , part 10, pp. 2425 - 2429

Transmission electron micrograph of negatively stained cells of strain J3A-127 .

Differential carbon source utilization between strain J3A-127 and the type strains of closely related species of the genus



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