1887

Abstract

A Gram-stain-negative, aerobic, rod-shaped bacterium, designated strain J3-A127, was isolated from the roots of fresh rice plants (). Cells were non-motile and no flagellum was detected. Comparison of 16S rRNA gene sequences indicated that the strain was phylogenetically related to species of the genus , with closest similarity to Alt 505 (96.4 %). The low levels of 16S rRNA gene sequence similarity (<90 %) found between the , , and gene sequences of strain J3-A127 and the type strains of recognized species of the genus also indicated that it represented a separate species. The temperature range for growth was 10–40 °C (optimum around 28 °C) and the pH range was 6.0–11.0 (optimum pH 7.0–8.0). Strain J3-A127 tolerated NaCl concentrations up to 5.0 % (w/v). The strain was catalase- and oxidase-positive. The main cellular fatty acids were summed feature 8 (Cω7 and/or Cω6; 46.7 %). The DNA G+C content of strain J3-A127 was 59.5 mol%. Strain J3-A127 did not form any nodules on four different legumes and the and genes were not detected by PCR. According to physiological and biochemical characteristics and genotypic data, strain J3-A127 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is J3-A127 ( = ACCC 10380 = KCTC 23294).

Funding
This study was supported by the:
  • , National Natural Science Foundation of China , (Award 30900001)
  • , Special scientific research funds for commonwealth agricultural industry , (Award 200803029)
  • , special foundation for central academy of the Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences , (Award 2009-10)
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.026146-0
2011-10-01
2021-01-24
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/61/10/2425.html?itemId=/content/journal/ijsem/10.1099/ijs.0.026146-0&mimeType=html&fmt=ahah

References

  1. Berge O., Lodhi A., Brandelet G., Santaella C., Roncato M. A., Christen R., Heulin T., Achouak W. 2009; Rhizobium alamii sp. nov., an exopolysaccharide-producing species isolated from legume and non-legume rhizospheres. Int J Syst Evol Microbiol 59:367–372 [CrossRef][PubMed]
    [Google Scholar]
  2. Chun J., Lee J.-H., Jung Y., Kim M., Kim S., Kim B. K., Lim Y. W. 2007; EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57:2259–2261 [CrossRef][PubMed]
    [Google Scholar]
  3. Dong X. Z., Cai M. Y. 2001 Determinative Manual for Routine Bacteriology Beijing: Science Press; (in Chinese)
    [Google Scholar]
  4. Euzéby J. P. 1997; List of Bacterial Names with Standing in Nomenclature: a folder available on the Internet. Int J Syst Bacteriol 47:590–592 [CrossRef][PubMed]
    [Google Scholar]
  5. Frank B. 1889; Über die Pilzsymbiose der Leguminosen. Ber Dtsch Bot Ges 7:332–346 (in German)
    [Google Scholar]
  6. Gao J. L., Sun J. G., Li, Y., Wang E. T., Chen W. X. 1994; Numerical taxonomy and DNA relatedness of tropical rhizobia isolated from Hainan Province, China. Int J Syst Bacteriol 44:151–158 [CrossRef][PubMed]
    [Google Scholar]
  7. García-Fraile P., Rivas R., Willems A., Peix A., Martens M., Martínez-Molina E., Mateos P. F., Velázquez E. 2007; Rhizobium cellulosilyticum sp. nov., isolated from sawdust of Populus alba . Int J Syst Evol Microbiol 57:844–848 [CrossRef][PubMed]
    [Google Scholar]
  8. Hunter W. J., Kuykendall L. D., Manter D. K. 2007; Rhizobium selenireducens sp. nov.: a selenite-reducing α-Proteobacteria isolated from a bioreactor. Curr Microbiol 55:455–460 [CrossRef][PubMed]
    [Google Scholar]
  9. Jordan D. C. 1984; Genus I. Rhizobium Frank 1889, 338AL . In Bergey’s Manual of Systematic Bacteriology vol. 1 pp. 235–242 Edited by Krieg N. R., Holt J. G. Baltimore: Williams & Wilkins;
    [Google Scholar]
  10. Laguerre G., Nour S. M., Macheret V., Sanjuan J., Drouin P., Amarger N. 2001; Classification of rhizobia based on nodC and nifH gene analysis reveals a close phylogenetic relationship among Phaseolus vulgaris symbionts. Microbiology 147:981–993[PubMed]
    [Google Scholar]
  11. Lin D. X., Chen W. F., Wang F. Q., Hu D., Wang E. T., Sui X. H., Chen W. X. 2009; Rhizobium mesosinicum sp. nov., isolated from root nodules of three different legumes. Int J Syst Evol Microbiol 59:1919–1923 [CrossRef][PubMed]
    [Google Scholar]
  12. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from micro-organisms. J Mol Biol 3:208–218 [CrossRef]
    [Google Scholar]
  13. Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5:109–118 [CrossRef][PubMed]
    [Google Scholar]
  14. Martens M., Delaere M., Coopman R., De Vos P., Gillis M., Willems A. 2007; Multilocus sequence analysis of Ensifer and related taxa. Int J Syst Evol Microbiol 57:489–503 [CrossRef][PubMed]
    [Google Scholar]
  15. Peng G. X., Yuan Q. H., Li H. X., Zhang W., Tan Z. Y. 2008; Rhizobium oryzae sp. nov., isolated from the wild rice Oryza alta . Int J Syst Evol Microbiol 58:2158–2163 [CrossRef][PubMed]
    [Google Scholar]
  16. Quan Z. X., Bae H. S., Baek J. H., Chen W. F., Im W. T., Lee S. T. 2005; Rhizobium daejeonense sp. nov. isolated from a cyanide treatment bioreactor. Int J Syst Evol Microbiol 55:2543–2549 [CrossRef][PubMed]
    [Google Scholar]
  17. Raja P., Uma S., Sundaram S. 2006; Non-nodulating pink-pigmented facultative Methylobacterium sp. with a functional nifH gene. World J Microbiol Biotechnol 22:1381–1384 [CrossRef]
    [Google Scholar]
  18. Sasser M. 1990; Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc.;
  19. Squartini A., Struffi P., Döring H., Selenska-Pobell S., Tola E., Giacomini A., Vendramin E., Velázquez E., Mateos P. F. et al. 2002; Rhizobium sullae sp. nov. (formerly ‘Rhizobium hedysari’), the root-nodule microsymbiont of Hedysarum coronarium L. Int J Syst Evol Microbiol 52:1267–1276 [CrossRef][PubMed]
    [Google Scholar]
  20. Tamura K., Dudley J., Nei M., Kumar S. 2007; mega4: Molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol 24:1596–1599 [CrossRef][PubMed]
    [Google Scholar]
  21. Tan Z. Y., Kan F. L., Peng G. X., Wang E. T., Reinhold-Hurek B., Chen W. X. 2001; Rhizobium yanglingense sp. nov., isolated from arid and semi-arid regions in China. Int J Syst Evol Microbiol 51:909–914 [CrossRef][PubMed]
    [Google Scholar]
  22. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [CrossRef][PubMed]
    [Google Scholar]
  23. Tighe S. W., de Lajudie P., Dipietro K., Lindström K., Nick G., Jarvis B. D. 2000; Analysis of cellular fatty acids and phenotypic relationships of Agrobacterium, Bradyrhizobium, Mesorhizobium, Rhizobium and Sinorhizobium species using the Sherlock Microbial Identification System. Int J Syst Evol Microbiol 50:787–801 [CrossRef][PubMed]
    [Google Scholar]
  24. Ueda T., Suga Y., Yahiro N., Matsuguchi T. 1995; Remarkable N2-fixing bacterial diversity detected in rice roots by molecular evolutionary analysis of nifH gene sequences. J Bacteriol 177:1414–1417[PubMed]
    [Google Scholar]
  25. Vincent J. M. 1970; The cultivation, isolation and maintenance of rhizobia. In A Manual for the Practical Study of the Root-Nodule Bacteria pp. 1–13 Edited by Vincent J. M. Oxford: Blackwell Scientific;
    [Google Scholar]
  26. Zhang X. X., Sun L., Qiu F. B., McLean R. J. C., Jiang R. B., Song W. 2008; Rheinheimera tangshanensis sp. nov., a rice root-associated bacterium. Int J Syst Evol Microbiol 58:2420–2424 [CrossRef][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.026146-0
Loading
/content/journal/ijsem/10.1099/ijs.0.026146-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error