RT Journal Article SR Electronic(1) A1 Zhang, Xiaoxia A1 Sun, Lei A1 Ma, Xiaotong A1 Sui, Xin Hua A1 Jiang, RuiboYR 2011 T1 Rhizobium pseudoryzae sp. nov., isolated from the rhizosphere of rice JF International Journal of Systematic and Evolutionary Microbiology, VO 61 IS 10 SP 2425 OP 2429 DO https://doi.org/10.1099/ijs.0.026146-0 PB Microbiology Society, SN 1466-5034, AB A Gram-stain-negative, aerobic, rod-shaped bacterium, designated strain J3-A127T, was isolated from the roots of fresh rice plants (Oryza sativa). Cells were non-motile and no flagellum was detected. Comparison of 16S rRNA gene sequences indicated that the strain was phylogenetically related to species of the genus Rhizobium, with closest similarity to Rhizobium oryzae Alt 505T (96.4 %). The low levels of 16S rRNA gene sequence similarity (<90 %) found between the gyrB, atpD, recA and glnII gene sequences of strain J3-A127T and the type strains of recognized species of the genus Rhizobium also indicated that it represented a separate species. The temperature range for growth was 10–40 °C (optimum around 28 °C) and the pH range was 6.0–11.0 (optimum pH 7.0–8.0). Strain J3-A127T tolerated NaCl concentrations up to 5.0 % (w/v). The strain was catalase- and oxidase-positive. The main cellular fatty acids were summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6; 46.7 %). The DNA G+C content of strain J3-A127T was 59.5 mol%. Strain J3-A127T did not form any nodules on four different legumes and the nodD and nifH genes were not detected by PCR. According to physiological and biochemical characteristics and genotypic data, strain J3-A127T is considered to represent a novel species of the genus Rhizobium, for which the name Rhizobium pseudoryzae sp. nov. is proposed. The type strain is J3-A127T ( = ACCC 10380T = KCTC 23294T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.026146-0