1887

Abstract

Two extremely halophilic archaeal strains, designated TNN44 and TNN58, were isolated from Tainan marine solar saltern near Lianyungang city, Jiangsu province, China. Cells of the two strains were pleomorphic and Gram-stain-negative; colonies were red-pigmented. Strains TNN44 and TNN58 were able to grow at 20–50 °C (optimum 37 °C for both), in the presence of 1.4–5.1 M NaCl (optimum 3.4–3.9 M NaCl) and at pH 5.5–9.0 (optimum pH 6.5–7.0); neither strain required Mg for growth. Cells lysed in distilled water. On the basis of 16S rRNA gene sequence analysis, strains TNN44 and TNN58 were related closely to RO2-11 (96.2 and 97.2 % similarity, respectively). The polar lipids of the two strains were phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, phosphatidylglycerol sulfate (trace), and one major glycolipid and one minor glycolipid chromatographically identical to sulfated mannosyl glucosyl diether and mannosyl glucosyl diether, respectively; other trace unidentified lipids were also detected. The DNA G+C content of strains TNN44 and TNN58 was 64.0 and 62.0 mol%, respectively. The level of DNA–DNA relatedness between strains TNN44 and TNN58 was 37.2 %, and these two strains showed a low level of DNA–DNA relatedness with RO2-11 (40.6 and 44.4 %, respectively). Two novel species of the genus are proposed to accommodate these two strains, sp. nov. (type strain TNN44  = CGMCC 1.10119  = JCM 16426) and sp. nov. (type strain TNN58  = CGMCC 1.10121  = JCM 16428).

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2011-04-01
2019-10-20
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Phase-contrast micrographs of cells of strain TNN44 (a) and strain TNN58 (b). Bars, 2 µm.

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Analysis of the polar lipid composition of strains TNN44 and TNN58 by using TLC. (a–e) Two-dimensional TLC of strain TNN44 (a, c), strain TNN58 (b, d), RO2-11 (e) and JCM 14791 (f). (g) One-dimensional TLC. Lanes: 1, CGMCC 1.2367 (=ATCC 33170); 2, RO2-11 ; 3, strain TNN44 ; 4, strain TNN58 ; 5, JCM 14791 . Circled spots are minor polar lipids of haloarchaeal strains tested. PG, phosphatidylglycerol; PGP-Me, phosphatidylglycerol phosphate methyl ester; PGS, phosphatidylglycerol sulfate; GL, glycolipid; DGD-1, mannosyl glucosyl diether; S-DGD-1, sulfated mannosyl glucosyl diether; F, first dimension of TLC; S, second dimension of TLC.

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Phylogenetic tree derived from maximum-parsimony analysis based on 16S rRNA gene sequences showing the relationship between strains TNN44 and TNN58 and other close relatives within the family . Bootstrap values (percentages) are based on 1000 replicates and are shown for branches with more than 50 % support. Bar, 50 expected changes per 1000 nt positions.

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