1887

Abstract

Bdellovibrios are unique, predatory bacteria with an intraperiplasmic growth and multiplication phase within their prey, which consists of many Gram-negative bacteria. Until recently, all bacteria that exhibited these traits were included in the genus . However, analysis of 16S rDNA sequences and other studies have demonstrated substantial genotypic, phenotypic and ecotypic diversity among the organisms in this genus ( Baer ., 2000 ; Snyder ., 2002 ). This has resulted in reclassification of and into the newly constructed genus ( Baer ., 2000 ). In this study, examination of marine isolates of (designated SJ, AQ and JS5) has revealed them to be related more closely to the newly designated genus . Phylogenetic analysis of 16S rRNA gene sequences revealed that marine isolates SJ, AQ and JS5 clustered in a separate clade from 100 as part of the clade that contains spp., indicating a much closer taxonomic relationship to the latter. DNA–DNA hybridization experiments also demonstrated <5 % similarity between 100 and the marine isolates. Distinct differences between the salt-water group and spp. were also observed by determination of DNA G+C content, salinity growth testing and antibiotic sensitivity analysis. On the basis of the results from the studies described above, it is proposed that marine isolates SJ (=ATCC BAA-682=DSM 15412) and JS5 (=ATCC BAA-684=DSM 15409) should be classified within the genus as the type strains of sp. nov. and sp. nov., respectively.

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2004-07-01
2021-10-26
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