1887

Abstract

Analysis of the morphological and genotypic properties of three obligately thermophilic strains of and , originally described as green non-sulfur bacteria, indicates that these taxa belong to the subdivision of the . EM of the cell wall clearly showed morphology typical of Gram-positive bacteria. A comparison of 16S rRNA gene sequences, including signature nucleotide pairs and secondary structural features considered diagnostic for the subclass , revealed that the three strains of were highly similar and should be considered as members of group 2 of this subclass, represented up to now only by uncultured organisms.

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2003-03-01
2021-03-08
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References

  1. Altschul S. F., Madden T. L., Schaffer A. A., Zhang J., Zhang Z., Miller W., Lipman D. J. 1997; Gapped blast and psi-blast: a new generation of protein database search programs. Nucleic Acids Res 25:3389–3402 [CrossRef]
    [Google Scholar]
  2. Anzai Y., Kim H., Park J.-Y., Wakabayashi H., Oyaizu H. 2000; Phylogenetic affiliation of the pseudomonads based on 16S rRNA sequence. Int J Syst Evol Microbiol 50:1563–1589 [CrossRef]
    [Google Scholar]
  3. Holmes A. J., Bowyer J., Holley M. P., O'Donoghue M., Montgomery M., Gillings M. R. 2000; Diverse, yet-to-be-cultured members of the Rubrobacter subdivision of the Actinobacteria are widespread in Australian arid soils. FEMS Microbiol Ecol 33:111–120 [CrossRef]
    [Google Scholar]
  4. Merkel G. J., Stapleton S. S., Perry J. J. 1978; Isolation and peptidoglycan of Gram-negative hydrocarbon-utilizing thermophilic bacteria. J Gen Microbiol 109:141–148 [CrossRef]
    [Google Scholar]
  5. Perry J. J. 1984; Genus Thermomicrobium Jackson, Ramaley and Meinschein 1973, 34AL. In Bergey's Manual of Systematic Bacteriology vol. 1 pp 338–339Edited by Krieg N. R., Holt J. G. Baltimore: Williams & Wilkins;
    [Google Scholar]
  6. Phillips W. E. Jr, Perry J. J. 1976; Thermomicrobium fosteri sp. nov., a hydrocarbon-utilizing obligate thermophile. Int J Syst Bacteriol 26:220–225 [CrossRef]
    [Google Scholar]
  7. Rheims H., Spröer C., Rainey F. A., Stackebrandt E. 1996; Molecular biological evidence for the occurrence of uncultured members of the actinomycete line of descent in different environments and geographical locations. Microbiology 142:2863–2870 [CrossRef]
    [Google Scholar]
  8. Stackebrandt E., Rainey F. A., Ward-Rainey N. L. 1997; Proposal for a new hierarchic classification system, Actinobacteria classis nov. Int J Syst Bacteriol 47:479–491 [CrossRef]
    [Google Scholar]
  9. Zarilla K. A., Perry J. J. 1984; Thermoleophilum album , gen. nov. and sp. nov., a bacterium obligate for thermophily and n -alkane substrates. Arch Microbiol 137:286–290 [CrossRef]
    [Google Scholar]
  10. Zarilla K. A., Perry J. J. 1986a; Deoxyribonucleic acid homology and other comparisons among obligately thermophilic hydrocarbonoclastic bacteria, with a proposal for Thermoleophilum minutum sp. nov. Int J Syst Bacteriol 36:13–16 [CrossRef]
    [Google Scholar]
  11. Zarilla K. A., Perry J. J. 1986b; Thermoleophilum album gen. nov., sp. nov. In Validation of the Publication of New Names and New Combinations Previously Effectively Published Outside the IJSB List no. 20. Int J Syst Bacteriol 36:354–356 [CrossRef]
    [Google Scholar]
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