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Volume 53,
Issue 2,
2003
Volume 53, Issue 2, 2003
- Validation List No. 90
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Validation of publication of new names and new combinations previously effectively published outside the IJSEM
The purpose of this announcement is to effect the valid publication of the following new names and new combinations under the procedure described previously [Int J Syst Bacteriol 27(3), iv (1977)]. Authors and other individuals wishing to have new names and/or combinations included in future lists should send two copies of the pertinent reprint or photocopies thereof to the IJSEM Editorial Office for confirmation that all of the other requirements for valid publication have been met. It should be noted that the date of valid publication of these new names and combinations is the date of publication of this list, not the date of the original publication of the names and combinations. The authors of the new names and combinations are as given below, and these authors' names will be included in the author index of the present issue and in the volume author index. Inclusion of a name on these lists validates the name and thereby makes it available in bacteriological nomenclature. The inclusion of a name on this list is not to be construed as taxonomic acceptance of the taxon to which the name is applied. Indeed, some of these names may, in time, be shown to be synonyms, or the organisms may be transferred to another genus, thus necessitating the creation of a new combination.
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- Notification List
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Notification that new names and new combinations have appeared in volume 52, part 6, of the IJSEM
This listing of names published in a previous issue of the IJSEM is provided as a service to bacteriology to assist in the recognition of new names and new combinations. This procedure was proposed by the Judicial Commission [Minute II(ii), Int J Syst Bacteriol 41 (1991), p. 185]. The names given herein have priority according to the page number of the IJSEM on which they were proposed.
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- New Taxa
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- Other Bacteria
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Flavobacterium limicola sp. nov., a psychrophilic, organic-polymer-degrading bacterium isolated from freshwater sediments
Three novel strains of cold-adapted bacteria, ST-82T, ST-10 and ST-92, were isolated from freshwater sediments. These three isolates were very similar to each other in phenotypic and chemotaxonomic traits, as well as in 16S rDNA sequence. The strains were Gram-negative, elongated filament-like rods that formed bright yellow colonies. They showed neither flexirubin pigments nor gliding motility. The strains were able to hydrolyse casein, gelatin, starch, agar, aesculin, urea, uric acid and tyrosine. They also lysed cells of Escherichia coli and Pseudomonas putida. The temperature range for growth was 0–25 °C, with optimum growth occurring at 15–20 °C. For all isolates, protease secretion increased as temperature decreased. Sodium chloride inhibited their growth, although the strains tolerated up to 1·5 % (w/v) NaCl. Menaquinone-6 was the major respiratory quinone. The major cellular fatty acids were C15 : 0, iso-C15 : 0, anteiso-C15 : 0, C15 : 1, iso-C15 : 1, C16 : 1 ω7cis, iso-C16 : 1, iso-C17 : 1, iso-C15 : 0 3-OH and iso-C16 : 0 3-OH. The DNA G+C content was 34·0–34·8 mol%. Phylogenetic analysis based on 16S rDNA sequences suggested that the strains belonged to the genus Flavobacterium and were closely related to Flavobacterium xanthum and Flavobacterium frigidarium, with sequence similarities of 96·9 and 96·3 %, respectively. In physiological and biochemical analyses, the isolates were differentiated from all known members of the genus Flavobacterium. The name Flavobacterium limicola is proposed for these novel strains, and the type strain is ST-82T (=JCM 11473T =DSM 15094T).
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- Proteobacteria
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Elevation of three subspecies of Pectobacterium carotovorum to species level: Pectobacterium atrosepticum sp. nov., Pectobacterium betavasculorum sp. nov. and Pectobacterium wasabiae sp. nov.
More LessA collection of 42 strains belonging to the five subspecies of Pectobacterium carotovorum (subspecies atrosepticum, betavasculorum, carotovorum, odoriferum and wasabiae) and 11 reference and type strains of biovars of Pectobacterium chrysanthemi, Pectobacterium cacticidum and Brenneria paradisiaca were studied by DNA–DNA hybridization, numerical taxonomy of 120 phenotypic characteristics, serology and new phylogenetic analysis of previously reported sequences from a database of aligned 16S rDNA sequences. The P. carotovorum subspecies formed a clade according to neighbour-joining methods, but they formed two paraphyletic clusters according to maximum-likelihood and maximum-parsimony. However, phylogenetic analysis of 16S rDNA sequences alone is not sufficient to justify generic differentiation and therefore, it is proposed to retain the P. carotovorum subspecies in the genus Pectobacterium. The strains of P. carotovorum were distributed in four genomospecies: genomospecies 1, harbouring all strains of subsp. atrosepticum, genomospecies 2, including the strains of subsp. betavasculorum isolated from sugar beet, sunflower, potato, hyacinth and artichoke, genomospecies 3, clustering all strains of subsp. wasabiae isolated from wasabi in Japan, and genomospecies 4, gathering together strains of subsp. carotovorum and strains of subsp. odoriferum. Four strains of P. carotovorum subsp. carotovorum remained unclustered. Biochemical criteria, deduced from a numerical taxonomy study of phenotypic characteristics and serological reactions, allowed discrimination of strains belonging to the four genomospecies. Thus, it is proposed that three genomospecies be elevated to species level as Pectobacterium atrosepticum sp. nov. (type strain CFBP 1526T=LMG 2386T =NCPPB 549T =ICMP 1526T), Pectobacterium betavasculorum sp. nov. (type strain CFBP 2122T=LMG 2464T =NCPPB 2795T =ICMP 4226T) and Pectobacterium wasabiae sp. nov. (type strain CFBP 3304T=LMG 8404T =NCPPB 3701T =ICMP 9121T). Only two subspecies are maintained within P. carotovorum, subsp. carotovorum (type strain CFBP 2046T=LMG 2404T =NCPPB 312T =ICMP 5702T) and subsp. odoriferum (type strain CFBP 1878T=LMG 5863T =NCPPB 3839T =ICMP 11553T), for which discriminating tests are available.
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Reclassification of ‘Pseudomonas fluorescens subsp. cellulosa’ NCIMB 10462 (Ueda et al. 1952) as Cellvibrio japonicus sp. nov. and revival of Cellvibrio vulgaris sp. nov., nom. rev. and Cellvibrio fulvus sp. nov., nom. rev.
More Less‘Pseudomonas fluorescens subsp. cellulosa’ NCIMB 10462 has been demonstrated by a polyphasic taxonomic approach to be a member of the genus Cellvibrio. 16S rDNA sequence analysis suggests that this is the only genus that could accept this specimen. The sequence is 95·5 % similar to that of Cellvibrio mixtus subsp. mixtus ACM 2601T (the type strain of the type species of the genus), which is its closest relation. The genomic DNA G+C content was determined to be 53·3 mol%, which is similar to the values obtained for the validly described Cellvibrio species. DNA–DNA hybridization experiments have shown that strain NCIMB 10462T (=NCDO 2697T) represents a novel species; therefore, it is proposed that it be designated as the type strain of the novel species Cellvibrio japonicus sp. nov. This study also used 16S rDNA analysis, DNA–DNA hybridization experiments and phenotypic testing to revive the species Cellvibrio vulgaris sp. nov., nom. rev. and Cellvibrio fulvus sp. nov., nom. rev. C. vulgaris NCIMB 8633T (=LMG 2848T) and C. fulvus NCIMB 8634T (=LMG 2847T) are the proposed type strains.
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Microvirga subterranea gen. nov., sp. nov., a moderate thermophile from a deep subsurface Australian thermal aquifer
More LessA strictly aerobic bacterium, strain FaiI4T, was isolated from free-flowing geothermal waters of a bore (bore register no. 3768) tapping the Great Artesian Basin of Australia. The non-sporulating, Gram-negative cells of strain FaiI4T produced light-pink colonies, were rod-shaped (1×1·5–4 µm) and were motile by a single polar flagellum. Strain FaiI4T grew optimally at 41 °C at a pH of 7·0 and had an absolute requirement for yeast extract. The strain grew on casein hydrolysate, tryptone, gelatin, xylose and acetate in a medium supplemented with 0·06 or 0·006 % yeast extract. Weak acid production was detected from glucose and arabinose. Catalase was produced. Nitrite was produced from nitrate. Strain FaiI4T was sensitive to antibiotics that inhibit growth of bacteria. The G+C content was 63·5±0·5 mol%. Strain FaiI4T was a member of the class ‘Alphaproteobacteria’, phylum Proteobacteria, placed almost equidistantly between Methylobacterium species, Chelatococcus asaccharovorans and Bosea thiooxidans (similarity value of 93 %) as its nearest phylogenetic relatives. Phylogenetic and phenotypic evidence suggest that strain FaiI4T (=ATCC BAA-295T =DSM 14364T) should be placed as the type strain of a species in a newly created genus, for which the name Microvirga subterranea gen. nov., sp. nov. is proposed.
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Idiomarina baltica sp. nov., a marine bacterium with a high optimum growth temperature isolated from surface water of the central Baltic Sea
More LessTwo bacterial strains isolated from the Baltic Sea, OS145T and OS146, were characterized on the basis of their physiological and biochemical features, their fatty acid profiles and their phylogenetic position based on 16S rDNA sequence analyses. The strains were isolated from the upper oxic water column of the central Baltic Sea. Phylogenetic analyses of the 16S rDNA gene sequences revealed a clear affiliation of the novel strains with members of the genus Idiomarina, of the Gammaproteobacteria. Closest sequence similarity was seen with Idiomarina abyssalis and Idiomarina zobellii (95–96 %). The mean G+C content of the DNA of strains OS145T and OS146 was 49·7 mol%. Both strains were non-pigmented, Gram-negative, polarly flagellated organisms that were strictly aerobic. Growth of the strains was observed at salinities ranging from 0·8 to 10 % NaCl. Temperature range for growth was rather broad and high for marine bacteria: both strains grew between 8 and 46 °C, showed good growth between 20 and 44 °C, and had an optimum between 30 and 40 °C. The fatty acids of the two strains were dominated by iso-branched fatty acids (54–80 %), with a high abundance of C15 : 0 iso (36 %), C16 : 1 ω7c , C17 : 0 iso and C17 : 1 iso ω9c . Growth temperature (8–40 °C) influenced the fatty acid composition of the strains in a way that the content of iso-branching fatty acids increased with increasing temperatures, while the mono-unsaturated fatty acids increased with decreasing temperatures. Salinity (1·7–10 % NaCl) had only a minor effect on the fatty acid composition. According to their morphology, physiology, fatty acid composition and 16S rDNA sequences, strains OS145T and OS146 fitted well into the genus Idiomarina, but could be easily distinguished from the recognized species of the genus. Because of their unique nature, it is proposed that the strains isolated from the Baltic Sea represent a novel species, for which the name Idiomarina baltica (type strain OS145T=DSM 15154T =LMG 21691T) is proposed.
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Psychrobacter pulmonis sp. nov., isolated from the lungs of lambs
Unusual Gram-negative, catalase- and oxidase-positive, coccus-shaped bacteria isolated from the lungs of two lambs were characterized by phenotypic and molecular-genetic methods. Comparative 16S rRNA gene sequencing studies demonstrated that the unknown isolates were genealogically highly related to each other (99·8 % sequence similarity) and represent a novel subline within the genus Psychrobacter. The unknown bacterium was phylogenetically closely related to, but distinct from, Psychrobacter phenylpyruvicus, Psychrobacter immobilis, Psychrobacter glacincola and Psychrobacter urativorans. The novel Psychrobacter isolates were readily distinguished from all other Psychrobacter species and other Gram-negative, oxidase-positive bacteria usually responsible for lung infections in sheep by physiological and biochemical tests. Based on molecular-genetic and phenotypic evidence, it is proposed that the unknown Psychrobacter isolates from lambs be classified as Psychrobacter pulmonis sp. nov. The type strain is strain S-606T (=CECT 5989T =CCUG 46240T).
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Paracoccus seriniphilus sp. nov., an l-serine-dehydratase-producing coccus isolated from the marine bryozoan Bugula plumosa
A novel marine Gram-negative, non-motile, non-spore-forming, aerobic bacterium, associated with the bryozoan Bugula plumosa, was isolated in a screening programme for strains containing enzymes able to convert the amino acid l-serine. Strain MBT-A4T produced l-serine dehydratase and was able to grow on l-serine as the sole carbon and nitrogen source. The nearest phylogenetic neighbour was Paracoccus marcusii, as determined by 16S rDNA sequence analysis (97·6 % similarity). The DNA–DNA reassociation value obtained for Paracoccus marcusii DSM11574T and MBT-A4T was 32·6 %. The major ubiquinone was Q-10. Based on genotypic, chemotaxonomic and physiological characteristics, a new species of the genus Paracoccus is proposed, Paracoccus seriniphilus sp. nov., the type strain being strain MBT-A4T (=DSM 14827T =CIP 107400T).
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Psychrobacter jeotgali sp. nov., isolated from jeotgal, a traditional Korean fermented seafood
More LessTwo Gram-negative, non-motile, non-spore-forming and moderately halophilic cocci (strains YKJ-103T and YKJ-105) were isolated from the traditional Korean fermented seafood, jeotgal. The two strains grew optimally at 25–30 °C and grew at 4 and 36 °C, but not above 37 °C. They grew in the presence of 0–10 % (w/v) NaCl with an optimum of 2–3 % (w/v) NaCl. Strains YKJ-103T and YKJ-105 were chemotaxonomically characterized by having ubiquinone-8 (Q-8) as the predominant isoprenoid quinone and C18 : 1 ω9c as the major fatty acid. The polar lipid analysis indicated the presence of diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. The DNA G+C contents of strains YKJ-103T and YKJ-105 were 44 and 43 mol%, respectively. Strains YKJ-103T and YKJ-105 showed no difference in their 16S rDNA sequences, and their mean level of DNA–DNA relatedness was 92·3 %. Phylogenetic analysis based on 16S rDNA sequences showed that the two strains form a distinct phylogenetic lineage within the cluster comprising Psychrobacter species. Strains YKJ-103T and YKJ-105 exhibited 16S rDNA similarities of 96·6 % with the type strain of Psychrobacter proteolyticus, the closest Psychrobacter species, and of 94·5–95·9 % with type strains of other Psychrobacter species. On the basis of phenotypic properties, phylogenetic and genomic data, strains YKJ-103T and YKJ-105 should be placed in the genus Psychrobacter as members of a new species, for which the name Psychrobacter jeotgali sp. nov. is proposed. The type strain of the new species is strain YKJ-103T (=KCCM 41559T =JCM 11463T).
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Taxonomic study of Cellvibrio strains and description of Cellvibrio ostraviensis sp. nov., Cellvibrio fibrivorans sp. nov. and Cellvibrio gandavensis sp. nov.
More LessThirty-one cellulolytic bacterial isolates from soils that were phenotypically very similar and phylogenetically highly related to Cellvibrio strains were further characterized using a polyphasic taxonomic approach. By using repetitive extragenic palindromic DNA-PCR fingerprinting, six different fingerprints could be recognized among the isolates. Representative strains and four reference strains of the genus Cellvibrio were used for DNA–DNA hybridization, which yielded eight DNA hybridization groups at a cut-off level of 70 % DNA binding. One group was formed by three isolates and Cellvibrio vulgaris LMG 2848T and a second group consisted of Cellvibrio mixtus strains ACM 2601T and ACM 2603. Two isolates and Cellvibrio fulvus LMG 2847T constituted single-member groups. For the remaining groups, three novel species are proposed: Cellvibrio fibrivorans sp. nov. (six strains, type strain LMG 18561T =ACM 5172T), Cellvibrio ostraviensis sp. nov. (eight strains, type strain LMG 19434T =ACM 5173T) and Cellvibrio gandavensis sp. nov. (12 strains, type strain LMG 18551T =ACM 5174T). The novel Cellvibrio species could be differentiated from each other and from C. mixtus, C. vulgaris and C. fulvus on the basis of phenotypic features, their fatty acid compositions and the G+C content of their DNA.
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Sphingopyxis chilensis sp. nov., a chlorophenol-degrading bacterium that accumulates polyhydroxyalkanoate, and transfer of Sphingomonas alaskensis to Sphingopyxis alaskensis comb. nov.
More LessThe taxonomic position of a chlorophenol-degrading bacterium, strain S37T, was investigated. The 16S rDNA sequence indicated that this strain belongs to the genus Sphingopyxis, exhibiting high sequence similarity to the 16S rDNA sequences of Sphingomonas alaskensis LMG 18877T (98·8 %), Sphingopyxis macrogoltabida LMG 17324T (98·2 %), Sphingopyxis terrae IFO 15098T (95 %) and Sphingomonas adhaesiva GIFU 11458T (92 %). These strains (except Sphingopyxis terrae IFO 15098T, which was not investigated) and the novel isolate accumulated polyhydroxyalkanoates consisting of 3-hydroxybutyric acid and 3-hydroxyvaleric acid from glucose as carbon source. The G+C content of the DNA of strain S37T was 65·5 mol%. The major cellular fatty acids of this strain were octadecenoic acid (18 : 1ω7c), heptadecenoic acid (17 : 1ω6c) and hexadecanoic acid (16 : 0). The results of DNA–DNA hybridization experiments and its physiological characteristics clearly distinguished the novel isolate from all known Sphingopyxis species and indicated that the strain represents a novel Sphingopyxis species. Therefore, the species Sphingopyxis chilensis sp. nov. is proposed, with strain S37T (=LMG 20986T =DSM 14889T) as the type strain. The transfer of Sphingomonas alaskensis to the genus Sphingopyxis as Sphingopyxis alaskensis comb. nov. is also proposed.
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Vibrio ruber sp. nov., a red, facultatively anaerobic, marine bacterium isolated from sea water
More LessA red, heterotrophic, marine bacterium, designated strain VR1T, was isolated from a sea-water sample collected in the shallow coastal region of Keelung, Taiwan. Cells of the novel strain were facultatively anaerobic, Gram-negative rods that were motile by means of a polar flagellum. The strain grew optimally at 25–30 °C and pH 6–7. Growth required the presence of NaCl, the optimal concentration being about 2 %. The red pigment produced by the cells was identified as prodigiosin. Strain VR1T grew anaerobically by fermenting glucose and other carbohydrates and producing acids and gases. The strain did not require either vitamins or other organic growth factors for growth. It contained 2-OH-16 : 0 and 3-OH-14 : 0 as the major cellular fatty acids. The DNA G+C content was 45·8 mol%. Phenotypic and chemotaxonomic characterization indicated that strain VR1T represents a novel species in the genus Vibrio. Strain VR1T is phenotypically similar to Vibrio gazogenes. However, the reduction of nitrate to nitrite, the ability to utilize d-arabinose, melibiose and l-glycine as sole carbon sources, the inability to utilize sorbitol as a sole carbon source, resistance to O/129 and susceptibility to erythromycin and novobiocin allow differentiation between V. gazogenes and strain VR1T. The name Vibrio ruber sp. nov. is proposed for the novel species, with strain VR1T (=CCRC 17186T =JCM 11486T) as the type strain.
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Shewanella marinintestina sp. nov., Shewanella schlegeliana sp. nov. and Shewanella sairae sp. nov., novel eicosapentaenoic-acid-producing marine bacteria isolated from sea-animal intestines
More LessThree novel Shewanella species are described on the basis of phenotypic, chemotaxonomic and phylogenetic studies. A total of six novel halophilic, aerobic organisms with the ability to produce eicosapentaenoic acid (EPA) were isolated from various sea animals in Japan. Cells of all six isolates were Gram-negative, rod-shaped and motile by means of polar flagella. They were able to produce large amounts of EPA (about 20 % of the total fatty acids) and had isoprenoid quinones Q-7 and Q-8 as major components. Analysis of the nearly complete 16S rRNA gene sequences of the novel isolates showed that they are very close phylogenetically (sequence similarity >99 %) and the closest species was Shewanella pealeana, with 97 % sequence similarity. However, analysis of gyrB sequences indicated that the novel isolates were divided into three groups at sufficient phylogenetic distance to indicate that they are different species (<90 % sequence similarity). DNA–DNA hybridization experiments supported this conclusion. The first group (three strains) had positive reactions for lipase, DNase, ONPG and trimethylamine oxide (TMAO) reduction and had G+C contents of 43 mol% (determined by HPLC). The second group (two strains) was positive for urease, DNase, ONPG and TMAO reduction but not lipase. Their G+C content was 45 mol%. The third group (one strain) was negative for ONPG, DNase and TMAO reduction and had a G+C content of 43 mol%. Strains of the second group, but not those of the first or third groups, grew at 32 °C. On the basis of the polyphasic taxonomic data, the novel strains isolated from intestines of sea animals are placed in three novel species of the genus Shewanella: Shewanella marinintestina sp. nov. (type strain: JCM 11558T=LMG 21403T), Shewanella schlegeliana sp. nov. (type strain: JCM 11561T=LMG 21406T) and Shewanella sairae sp. nov. (type strain: JCM 11563T=LMG 21408T).
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Psychromonas profunda sp. nov., a psychropiezophilic bacterium from deep Atlantic sediments
A psychropiezophilic bacterium, strain 2825T (=LMG 21260T =JCM 11437T), isolated from deep Atlantic sediments at a depth of 2770 m and a temperature of 2 °C, was found by polyphasic analysis to represent a novel species of the genus Psychromonas, Psychromonas profunda sp. nov. It is a strict psychrophile and a moderate piezophile, whose degree of piezophily is increased markedly when the temperature is raised to 10 °C. The piezophily of P. profunda is intermediate between that of the type species, Psychromonas antarctica, which is not piezophilic, and that of Psychromonas kaikoae, which is an obligate piezophile.
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Moritella profunda sp. nov. and Moritella abyssi sp. nov., two psychropiezophilic organisms isolated from deep Atlantic sediments
Strains 2674T (=LMG 21259T =JCM 11435T) and 2693T (=LMG 21258T =JCM 11436T) were isolated from Atlantic sediments at a temperature of 2 °C and a depth of 2815 m off the West African coast. Polyphasic evidence indicates that the two strains belong to the genus Moritella and represent distinct species, for which the names Moritella profunda sp. nov. (for strain 2674T) and Moritella abyssi sp. nov. (for strain 2693T) are proposed. The moderate piezophily of the two organisms is intermediate between that of the type species, Moritella marina, which is not piezophilic, and Moritella yayanosii, an obligate piezophile. Both are strict psychrophiles with slightly different cardinal temperatures: at 0·1 MPa, maximal growth rates are observed at 2 °C (M. profunda) and 4 °C (M. abyssi) with maximum temperatures of 12 °C (M. profunda) or 14 °C (M. abyssi). The optimal pressure is lower than that at the site of isolation, and raising the temperature to 10 °C makes the organisms more piezophilic.
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Psychromonas arctica sp. nov., a novel psychrotolerant, biofilm-forming bacterium isolated from Spitzbergen
More LessUsing starch as a carbon source at a cultivation temperature of 4 °C, a number of Gram-negative, aerobic strains was isolated from sea-ice and sea-water samples collected at Spitzbergen in the Arctic. Analysis of the genetic diversity of the novel isolates by random amplification of polymorphic DNA (RAPD) and ERIC fingerprinting revealed a homogenic group of biofilm-forming bacteria that contained small extrachromosomal elements. As a representative of the group, strain Pull 5.3T, isolated from a sea-water sample, was used for detailed characterization. The results of phylogenetic analysis indicated that the newly isolated strain is a member of the γ-subclass of the Proteobacteria and belongs to the genus Psychromonas. On the basis of DNA–DNA hybridization experiments, chemotaxonomic studies and phenotypic characterization, strain Pull 5.3T (=CECT 5674T =DSM 14288T) clearly represents a novel species, for which the name Psychromonas arctica sp. nov. is proposed.
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Pannonibacter phragmitetus gen. nov., sp. nov., a novel alkalitolerant bacterium isolated from decomposing reed rhizomes in a Hungarian soda lake
Three alkalitolerant bacterial strains were isolated from the surface of decomposing rhizomes of reed [Phragmites australis (Cav.) Trin. et Steudel] in Lake Fertő (Hungary). Cells of the novel isolates were Gram-negative, motile rods and formed star-shaped aggregates. They were facultatively anaerobic and chemo-organotrophic. Bacteriochlorophyll a was not synthesized under aerobic conditions. The strains were catalase and oxidase positive, produced acid from d-glucose under aerobic and anaerobic conditions and reduced nitrate to nitrogen. They tolerated pH values from 7·0 to 11·0 and grew in the absence of NaCl as well as in up to 5 % (w/v) NaCl. The G+C content of the DNA was 64·6 mol% and the major isoprenoid quinone was Q-10. The dominant cellular fatty acid was C18 : 1 ω7c. The cell membrane contained phosphatidyl glycerol, diphosphatidyl glycerol, phosphatidyl ethanolamine, phosphatidyl serine and one unknown phospholipid as polar lipids. Polyphasic taxonomic characterization revealed that strain C6/19T is most closely related to the Stappia–Roseibium cluster in the α-subclass of the Proteobacteria (showing 95·8–93·6 % 16S rDNA sequence similarity). According to the phylogenetic and phenotypic evidence presented, a new genus and species is proposed, Pannonibacter phragmitetus gen. nov., sp. nov. The type strain is C6/19T (=DSM 14782T=NCAIM B02025T).
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Marinobacter litoralis sp. nov., a moderately halophilic bacterium isolated from sea water from the East Sea in Korea
More LessA Gram-negative, motile, non-spore-forming and moderately halophilic rod-shaped strain, SW-45T, was isolated from sea water of the East Sea in Korea. The organism grew optimally at 30–37 °C and grew at 4 and 46 °C. It grew in the presence of 0·5–18 % (v/w) NaCl, with an optimum of 2–7 % NaCl. Strain SW-45T was chemotaxonomically characterized by having ubiquinone-9 (Q-9) as the major respiratory lipoquinone and C16 : 0, C18 : 1 ω9c and C16 : 1 ω9c as the predominant fatty acids. The DNA G+C content was found to be 55 mol%. Phylogenetic analysis based on 16S rDNA sequences showed that strain SW-45T forms a coherent cluster with the clade comprising the two Marinobacter species. 16S rDNA sequence similarities between strain SW-45T and the Marinobacter species was 94·9 % to Marinobacter hydrocarbonoclasticus DSM 8798T and 95·3 % to Marinobacter aquaeolei DSM 11845T. Levels of DNA–DNA relatedness between strain SW-45T and the type strains of M. hydrocarbonoclasticus and M. aquaeolei were respectively 4·3 and 5·5 %. On the basis of phenotypic properties, phylogeny and genomic data, strain SW-45T (=KCCM 41591T =JCM 11547T) should be placed in the genus Marinobacter as a member of a novel species, for which the name Marinobacter litoralis sp. nov. is proposed. As part of this study, the major respiratory lipoquinone of M. hydrocarbonoclasticus and M. aquaeolei was also found to be Q-9.
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Shewanella fidelis sp. nov., isolated from sediments and sea water
Two marine bacterial strains, KMM 3582T and KMM 3589, isolated respectively from sediments of the South China Sea and sea water of the Sea of Japan, have been characterized. Comparative 16S rDNA sequence-based phylogenetic analysis placed the two strains in a separate branch of the γ-Proteobacteria within the members of the genus Shewanella. KMM 3582T showed the highest similarity (97·1 and 97·4 %, respectively) to Shewanella pealeana and Shewanella gelidimarina. The G+C contents of the DNAs of the two strains studied were 45·0 mol%. The level of DNA–DNA relatedness between the two strains was 82 %, indicating that they represent a single genospecies. These organisms were slightly pinkish, Gram-negative, polarly flagellated, facultatively anaerobic, mesophilic (with temperature range from 4 to 30 °C), neutrophilic and haemolytic and were able to degrade alginate, gelatin and DNA. The novel organisms were susceptible to gentamicin, lincomycin, oleandomycin, streptomycin and polymyxin. The predominant fatty acids were characteristic for shewanellae: 13 : 0-i, 15 : 0-i, 16 : 0 and 16 : 1ω7. Eicosapentaenoic acid, 20 : 5ω3, was not detected. Phylogenetic evidence, together with phenotypic characteristics, showed that the two bacteria constitute a novel species of the genus Shewanella. The name Shewanella fidelis sp. nov. is proposed, with the type strain KMM 3582T (=LMG 20551T =ATCC BAA-318T).
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Volumes and issues
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Volume 75 (2025)
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Volume 14 (1964)
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Volume 13 (1963)
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Volume 12 (1962)
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Volume 11 (1961)
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Volume 10 (1960)
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Volume 9 (1959)
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Volume 8 (1958)
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Volume 7 (1957)
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Volume 6 (1956)
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Volume 5 (1955)
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Volume 4 (1954)
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Volume 3 (1953)
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Volume 2 (1952)
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Volume 1 (1951)
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