1887

Abstract

A Gram-negative, yellow-pigmented, rod-shaped, strictly aerobic, non-flagellated, oxidase- and catalase-positive, marine bacterium, designated A2, was isolated from a marine sponge, , collected from the coast of Jeju Island, South Korea. Phylogenetic analysis based on nearly complete 16S rRNA gene sequences revealed that strain A2 was a member of the family . Its closest relatives were KMM 3901 and KMM 3553 (96.99 and 96.98 % 16S rRNA gene sequence similarity, respectively). DNA–DNA relatedness between strain A2 and KMM 3901 and KMM 3553 was 14.1 and 26.8 %, respectively. The dominant fatty acids (>5 %) of strain A2 were iso-C (33.9 %), iso-C 3-OH (20.8 %), iso-C G (10.5 %) and iso-C 3-OH (6.1 %). The DNA G+C content of strain A2 was 36.0 mol% and the major respiratory quinone was MK-6. On the basis of phenotypic and phylogenetic analysis, strain A2 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is A2 (=KCTC 22662 =DSM 22637).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.023499-0
2011-02-01
2019-10-18
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/61/2/330.html?itemId=/content/journal/ijsem/10.1099/ijs.0.023499-0&mimeType=html&fmt=ahah

References

  1. Bernardet, J.-F., Nakagawa, Y. & Holmes, B. ( 2002; ). Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 52, 1049–1070.[CrossRef]
    [Google Scholar]
  2. Bowman, J. P. ( 2000; ). Description of Cellulophaga algicola sp. nov., isolated from the surfaces of Antarctic algae, and reclassification of Cytophaga uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa comb. nov. Int J Syst Evol Microbiol 50, 1861–1868.
    [Google Scholar]
  3. Bruns, A., Rohde, M. & Berthe-Corti, L. ( 2001; ). Muricauda ruestringensis gen. nov., sp. nov., a facultatively anaerobic, appendaged bacterium from German North Sea intertidal sediment. Int J Syst Evol Microbiol 51, 1997–2006.[CrossRef]
    [Google Scholar]
  4. Chun, J., Lee, J.-H., Jung, Y., Kim, M., Kim, S., Kim, B. K. & Lim, Y.-W. ( 2007; ). EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57, 2259–2261.[CrossRef]
    [Google Scholar]
  5. Cole, J. R., Chai, B., Marsh, T. L., Farris, R. J., Wang, Q., Kulam, S. A., Chandra, S., McGarrell, D. M., Schmidt, T. M. & other authors ( 2003; ). The Ribosomal Database Project (RDP-II): previewing a new autoaligner that allows regular updates and the new prokaryotic taxonomy. Nucleic Acids Res 31, 442–443.[CrossRef]
    [Google Scholar]
  6. Cowan, S. T. & Steel, K. J. ( 1965; ). Manual for the Identification of Medical Bacteria. London. : Cambridge University Press.
    [Google Scholar]
  7. Cowan, S. T. & Steel, K. J. ( 1993; ). Manual for the Identification of Medical Bacteria, 3rd edn. Cambridge. : Cambridge University Press.
    [Google Scholar]
  8. Felsenstein, J. ( 2002; ). phylip (phylogeny inference package) version 3.6a. Distributed by the author. Department of Genome Sciences, University of Washington, Seattle, USA.
  9. Ivanova, E. P., Alexeeva, Y. V., Flavier, S., Wright, J. P., Zhukova, N. V., Gorshkova, N. M., Mikhailov, V. V., Nicolau, D. V. & Christen, R. ( 2004; ). Formosa algae gen. nov., sp. nov., a novel member of the family Flavobacteriaceae. Int J Syst Evol Microbiol 54, 705–711.[CrossRef]
    [Google Scholar]
  10. Johnson, J. L. ( 1994; ). Similarity analysis of rRNAs. In Methods for General and Molecular Bacteriology, pp. 683–700. Edited by Gerhardt, P., Murray, R. G. E., Wood, W. A. & Krieg, N. R.. Washington, DC. : American Society for Microbiology.
    [Google Scholar]
  11. Komagata, K. & Suzuki, K. ( 1987; ). Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19, 161–207.
    [Google Scholar]
  12. Lányí, B. ( 1987; ). Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19, 1–67.
    [Google Scholar]
  13. Marmur, J. & Doty, P. ( 1962; ). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5, 109–118.[CrossRef]
    [Google Scholar]
  14. Nedashkovskaya, O. I., Kim, S. B., Vancanneyt, M., Snauwaert, C., Lysenko, A. M., Rohde, M., Frolova, G. M., Zhukova, N. V., Mikhailov, V. V. & other authors ( 2006; ). Formosa agariphila sp. nov., a budding bacterium of the family Flavobacteriaceae isolated from marine environments, and emended description of the genus Formosa. Int J Syst Evol Microbiol 56, 161–167.[CrossRef]
    [Google Scholar]
  15. Thompson, J. D., Higgins, D. G. & Gibson, T. J. ( 1994; ). clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22, 4673–4680.[CrossRef]
    [Google Scholar]
  16. Yoon, J. H., Lee, K. C., Kho, Y. H., Kang, K. H., Kim, C. J. & Park, Y. H. ( 2002; ). Halomonas alimentaria sp. nov., isolated from jeotgal, a traditional Korean fermented seafood. Int J Syst Evol Microbiol 52, 123–130.
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.023499-0
Loading
/content/journal/ijsem/10.1099/ijs.0.023499-0
Loading

Data & Media loading...

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error