1887

Abstract

A Gram-negative, yellow-pigmented, rod-shaped, strictly aerobic, non-flagellated, oxidase- and catalase-positive, marine bacterium, designated A2, was isolated from a marine sponge, , collected from the coast of Jeju Island, South Korea. Phylogenetic analysis based on nearly complete 16S rRNA gene sequences revealed that strain A2 was a member of the family . Its closest relatives were KMM 3901 and KMM 3553 (96.99 and 96.98 % 16S rRNA gene sequence similarity, respectively). DNA–DNA relatedness between strain A2 and KMM 3901 and KMM 3553 was 14.1 and 26.8 %, respectively. The dominant fatty acids (>5 %) of strain A2 were iso-C (33.9 %), iso-C 3-OH (20.8 %), iso-C G (10.5 %) and iso-C 3-OH (6.1 %). The DNA G+C content of strain A2 was 36.0 mol% and the major respiratory quinone was MK-6. On the basis of phenotypic and phylogenetic analysis, strain A2 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is A2 (=KCTC 22662 =DSM 22637).

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2011-02-01
2021-10-28
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References

  1. Bernardet J.-F., Nakagawa Y., Holmes B. 2002; Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 52:1049–1070 [CrossRef]
    [Google Scholar]
  2. Bowman J. P. 2000; Description of Cellulophaga algicola sp. nov., isolated from the surfaces of Antarctic algae, and reclassification of Cytophaga uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa comb. nov. Int J Syst Evol Microbiol 50:1861–1868
    [Google Scholar]
  3. Bruns A., Rohde M., Berthe-Corti L. 2001; Muricauda ruestringensis gen. nov., sp. nov. a facultatively anaerobic, appendaged bacterium from German North Sea intertidal sediment. Int J Syst Evol Microbiol 511997–2006 [CrossRef]
    [Google Scholar]
  4. Chun J., Lee J.-H., Jung Y., Kim M., Kim S., Kim B. K., Lim Y.-W. 2007; EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57:2259–2261 [CrossRef]
    [Google Scholar]
  5. Cole J. R., Chai B., Marsh T. L., Farris R. J., Wang Q., Kulam S. A., Chandra S., McGarrell D. M., Schmidt T. M. other authors 2003; The Ribosomal Database Project (RDP-II): previewing a new autoaligner that allows regular updates and the new prokaryotic taxonomy. Nucleic Acids Res 31:442–443 [CrossRef]
    [Google Scholar]
  6. Cowan S. T., Steel K. J. 1965 Manual for the Identification of Medical Bacteria London: Cambridge University Press;
    [Google Scholar]
  7. Cowan S. T., Steel K. J. 1993 Manual for the Identification of Medical Bacteria, 3rd edn. Cambridge: Cambridge University Press;
    [Google Scholar]
  8. Felsenstein J. 2002 phylip (phylogeny inference package) version 3.6a. Distributed by the author. Department of Genome Sciences University of Washington; Seattle, USA:
    [Google Scholar]
  9. Ivanova E. P., Alexeeva Y. V., Flavier S., Wright J. P., Zhukova N. V., Gorshkova N. M., Mikhailov V. V., Nicolau D. V., Christen R. 2004; Formosa algae gen. , nov., sp. nov. a novel member of the family Flavobacteriaceae . Int J Syst Evol Microbiol 54705–711 [CrossRef]
    [Google Scholar]
  10. Johnson J. L. 1994; Similarity analysis of rRNAs. In Methods for General and Molecular Bacteriology. pp 683–700 Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;
  11. Komagata K., Suzuki K. 1987; Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19:161–207
    [Google Scholar]
  12. Lányí B. 1987; Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19:1–67
    [Google Scholar]
  13. Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5:109–118 [CrossRef]
    [Google Scholar]
  14. Nedashkovskaya O. I., Kim S. B., Vancanneyt M., Snauwaert C., Lysenko A. M., Rohde M., Frolova G. M., Zhukova N. V., Mikhailov V. V. other authors 2006; Formosa agariphila sp. nov., a budding bacterium of the family Flavobacteriaceae isolated from marine environments, and emended description of the genus Formosa . Int J Syst Evol Microbiol 56:161–167 [CrossRef]
    [Google Scholar]
  15. Thompson J. D., Higgins D. G., Gibson T. J. 1994; clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680 [CrossRef]
    [Google Scholar]
  16. Yoon J. H., Lee K. C., Kho Y. H., Kang K. H., Kim C. J., Park Y. H. 2002; Halomonas alimentaria sp. nov., isolated from jeotgal, a traditional Korean fermented seafood. Int J Syst Evol Microbiol 52:123–130
    [Google Scholar]
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