1887

Abstract

One of the spore-formers isolated from a spacecraft-assembly facility, belonging to the genus , is described on the basis of phenotypic characterization, 16S rDNA sequence analysis and DNA–DNA hybridization studies. It is a Gram-positive, facultatively anaerobic, rod-shaped eubacterium that produces endospores. The spores of this novel bacterial species exhibited resistance to UV, γ-radiation, HO and desiccation. The 16S rDNA sequence analysis revealed a clear affiliation between this strain and members of the low G+C . High 16S rDNA sequence similarity values were found with members of the genus and this was supported by fatty acid profiles. The 16S rDNA sequence similarity between strain FO-92 and DSM 5391 was very high. However, molecular characterizations employing small-subunit 16S rDNA sequences were at the limits of resolution for the differentiation of species in this genus, but DNA–DNA hybridization data support the proposal of FO-92 as sp. nov. (type strain is FO-92 =ATCC BAA-519 =DSM 15077).

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2003-01-01
2019-12-07
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