@article{mbs:/content/journal/ijsem/10.1099/ijs.0.02311-0, author = "Venkateswaran, Kasthuri and Kempf, Michael and Chen, Fei and Satomi, Masataka and Nicholson, Wayne and Kern, Roger", title = "Bacillus nealsonii sp. nov., isolated from a spacecraft-assembly facility, whose spores are γ-radiation resistant", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2003", volume = "53", number = "1", pages = "165-172", doi = "https://doi.org/10.1099/ijs.0.02311-0", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.02311-0", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "JPL-SAF, Jet Propulsion Laboratory Spacecraft Assembly Facility", keywords = "FAME, fatty acid methyl ester", abstract = "One of the spore-formers isolated from a spacecraft-assembly facility, belonging to the genus Bacillus, is described on the basis of phenotypic characterization, 16S rDNA sequence analysis and DNA–DNA hybridization studies. It is a Gram-positive, facultatively anaerobic, rod-shaped eubacterium that produces endospores. The spores of this novel bacterial species exhibited resistance to UV, γ-radiation, H2O2 and desiccation. The 16S rDNA sequence analysis revealed a clear affiliation between this strain and members of the low G+C Firmicutes. High 16S rDNA sequence similarity values were found with members of the genus Bacillus and this was supported by fatty acid profiles. The 16S rDNA sequence similarity between strain FO-92T and Bacillus benzoevorans DSM 5391T was very high. However, molecular characterizations employing small-subunit 16S rDNA sequences were at the limits of resolution for the differentiation of species in this genus, but DNA–DNA hybridization data support the proposal of FO-92T as Bacillus nealsonii sp. nov. (type strain is FO-92T =ATCC BAA-519T =DSM 15077T).", }