1887

Abstract

A taxonomic study was carried out on a novel bacterial strain, designated C115, isolated from a crude-oil-degrading consortium, enriched from deep-sea water of the Indian Ocean. Cells were Gram-negative short rods, mobile by means of a monopolar flagellum. Growth was observed at salinities of 0–7 % and at 10–43 °C. It was unable to degrade Tween 80 or gelatin. 16S rRNA gene sequence analysis showed that strain C115 was related most closely to NL21 (96.5 % similarity), KY 101 (96.4 %) and J3 (96.2 %). The predominant fatty acids were summed feature 8 (C 7 and/or C 6, 81.8 %) and C (7.0 %). The G+C content of the chromosomal DNA of strain C115 was 59 mol%. Based on its morphology, physiology and fatty acid composition together with 16S rRNA gene sequence comparisons, the novel strain most appropriately belongs to the genus , but can be distinguished readily from recognized species of the genus. Strain C115 is therefore considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is C115 (=RC92-7 =CCTCC AB 209298 =LMG 25540 =MCCC 1A01260).

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2011-02-01
2020-08-10
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References

  1. Ausubel F. M., Brent R., Kingston R. E., Moore D. D., Seidman J. G., Smith J. A., Struhl K. (editors) (1995). Short Protocols in Molecular Biology: a Compendium of Methods from Current Protocols in Molecular Biology, 3rd edn. New York: Wiley;
    [Google Scholar]
  2. Kang H. S., Yang H. L., Lee S. D. 2009; Nitratireductor kimnyeongensis sp. nov., isolated from seaweed. Int J Syst Evol Microbiol 59:1036–1039 [CrossRef]
    [Google Scholar]
  3. Kim K.-H., Roh S. W., Chang H.-W., Nam Y.-D., Yoon J.-H., Jeon C. O., Oh H.-M., Bae J.-W. 2009; Nitratireductor basaltis sp. nov., isolated from black beach sand. Int J Syst Evol Microbiol 59:135–138 [CrossRef]
    [Google Scholar]
  4. Labbé N., Parent S., Villemur R. 2004; Nitratireductor aquibiodomus gen. nov., sp. nov., a novel α -proteobacterium from the marine denitrification system of the Montreal Biodome (Canada). Int J Syst Evol Microbiol 54:269–273 [CrossRef]
    [Google Scholar]
  5. Lai Q., Yuan J., Gu L., Shao Z. 2009; Marispirillum indicum gen. nov., sp. nov. isolated from a deep-sea environment. Int J Syst Evol Microbiol 59:1278–1281 [CrossRef]
    [Google Scholar]
  6. Liu C., Shao Z. 2005; Alcanivorax dieselolei sp. nov., a novel alkane-degrading bacterium isolated from sea water and deep-sea sediment. Int J Syst Evol Microbiol 55:1181–1186 [CrossRef]
    [Google Scholar]
  7. Mesbah M., Whitman W. B. 1989; Measurement of deoxyguanosine/thymidine ratios in complex mixtures by high-performance liquid chromatography for determination of the mole percentage guanine+cytosine of DNA. J Chromatogr 479:297–306 [CrossRef]
    [Google Scholar]
  8. Rzhetsky A., Nei M. 1992; A simple method for estimating and testing minimum-evolution trees. Mol Biol Evol 9:945–967
    [Google Scholar]
  9. Rzhetsky A., Nei M. 1993; Theoretical foundation of the minimum-evolution method of phylogenetic inference. Mol Biol Evol 10:1073–1095
    [Google Scholar]
  10. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  11. Sasser M. 1990; Identification of bacteria by gas chromatography of cellular fatty acids , MIDI Technical Note, 101 . Newark, DE: MIDI;
    [Google Scholar]
  12. Tamura K., Dudley J., Nei M., Kumar S. 2007; mega4: molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol 24:1596–1599 [CrossRef]
    [Google Scholar]
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