1887

Abstract

A taxonomic study was carried out on a novel bacterial strain, designated C115, isolated from a crude-oil-degrading consortium, enriched from deep-sea water of the Indian Ocean. Cells were Gram-negative short rods, mobile by means of a monopolar flagellum. Growth was observed at salinities of 0–7 % and at 10–43 °C. It was unable to degrade Tween 80 or gelatin. 16S rRNA gene sequence analysis showed that strain C115 was related most closely to NL21 (96.5 % similarity), KY 101 (96.4 %) and J3 (96.2 %). The predominant fatty acids were summed feature 8 (C 7 and/or C 6, 81.8 %) and C (7.0 %). The G+C content of the chromosomal DNA of strain C115 was 59 mol%. Based on its morphology, physiology and fatty acid composition together with 16S rRNA gene sequence comparisons, the novel strain most appropriately belongs to the genus , but can be distinguished readily from recognized species of the genus. Strain C115 is therefore considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is C115 (=RC92-7 =CCTCC AB 209298 =LMG 25540 =MCCC 1A01260).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.022962-0
2011-02-01
2019-10-16
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/61/2/295.html?itemId=/content/journal/ijsem/10.1099/ijs.0.022962-0&mimeType=html&fmt=ahah

References

  1. Ausubel, F. M., Brent, R., Kingston, R. E., Moore, D. D., Seidman, J. G., Smith, J. A. & Struhl, K. (editors) ( 1995; ). Short Protocols in Molecular Biology: a Compendium of Methods from Current Protocols in Molecular Biology, 3rd edn. New York. : Wiley.
    [Google Scholar]
  2. Kang, H. S., Yang, H. L. & Lee, S. D. ( 2009; ). Nitratireductor kimnyeongensis sp. nov., isolated from seaweed. Int J Syst Evol Microbiol 59, 1036–1039.[CrossRef]
    [Google Scholar]
  3. Kim, K.-H., Roh, S. W., Chang, H.-W., Nam, Y.-D., Yoon, J.-H., Jeon, C. O., Oh, H.-M. & Bae, J.-W. ( 2009; ). Nitratireductor basaltis sp. nov., isolated from black beach sand. Int J Syst Evol Microbiol 59, 135–138.[CrossRef]
    [Google Scholar]
  4. Labbé, N., Parent, S. & Villemur, R. ( 2004; ). Nitratireductor aquibiodomus gen. nov., sp. nov., a novel α-proteobacterium from the marine denitrification system of the Montreal Biodome (Canada). Int J Syst Evol Microbiol 54, 269–273.[CrossRef]
    [Google Scholar]
  5. Lai, Q., Yuan, J., Gu, L. & Shao, Z. ( 2009; ). Marispirillum indicum gen. nov., sp. nov., isolated from a deep-sea environment. Int J Syst Evol Microbiol 59, 1278–1281.[CrossRef]
    [Google Scholar]
  6. Liu, C. & Shao, Z. ( 2005; ). Alcanivorax dieselolei sp. nov., a novel alkane-degrading bacterium isolated from sea water and deep-sea sediment. Int J Syst Evol Microbiol 55, 1181–1186.[CrossRef]
    [Google Scholar]
  7. Mesbah, M. & Whitman, W. B. ( 1989; ). Measurement of deoxyguanosine/thymidine ratios in complex mixtures by high-performance liquid chromatography for determination of the mole percentage guanine+cytosine of DNA. J Chromatogr 479, 297–306.[CrossRef]
    [Google Scholar]
  8. Rzhetsky, A. & Nei, M. ( 1992; ). A simple method for estimating and testing minimum-evolution trees. Mol Biol Evol 9, 945–967.
    [Google Scholar]
  9. Rzhetsky, A. & Nei, M. ( 1993; ). Theoretical foundation of the minimum-evolution method of phylogenetic inference. Mol Biol Evol 10, 1073–1095.
    [Google Scholar]
  10. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  11. Sasser, M. ( 1990; ). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101 . Newark, DE. : MIDI.
    [Google Scholar]
  12. Tamura, K., Dudley, J., Nei, M. & Kumar, S. ( 2007; ). mega4: molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol 24, 1596–1599.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.022962-0
Loading
/content/journal/ijsem/10.1099/ijs.0.022962-0
Loading

Data & Media loading...

Supplements

Cellular fatty acid contents of strain C115 and the type strains of recognized species. [PDF](59 KB)

PDF

Transmission electron micrograph of a negatively stained cell of strain C115 grown on marine agar 2216 (BD) for 24 h. Bar, 0.5 µm.

IMAGE

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error