1887

Abstract

Strain A2 is an anaerobic, variably Gram-stain-positive, non-spore-forming, small and irregularly rod-shaped bacterium from the ruminal fluid of a sheep that has been described informally as a representative of ‘ (basonym ) ’. Three phenotypically similar bacterial strains (lac15, lac16 and lac31) were isolated in concert with lac18 from the mucosal jejunum of a pig. A phylogenetic analysis based on 16S rRNA gene sequences revealed that strains A2, lac15, lac16 and lac31 formed a genetically coherent group (100 % interstrain sequence similarity) within the bigeneric branch of the family , class . This group was most closely related to the type strains of the two recognized species, namely (sequence similarity of 96.85 %) and (sequence similarity of 97.20 %). The sequence similarity to the type strain of , the type species of the genus , was 92.33 %. Unlike those of and , outgrown colonies of strains A2, lac15, lac16 and lac31 were opaque and greyish-white with an umbonate elevation on solid culture media. The four novel strains were characterized as being well-adapted and presumably indigenous to the gastrointestinal tract of homoeothermic vertebrates: they were mesophilic, microaerotolerant, neutrophilic and acidotolerant, bile-resistant, mucin-utilizing and markedly peptidolytic lactic acid bacteria. The results of DNA–DNA hybridizations, cellular fatty acid analysis and other differential phenotypic (physiological and biochemical) tests confirmed that strains A2, lac15, lac16 and lac31 represent a novel species of the genus . On the basis of the genotypic and phenotypic results, we therefore describe sp. nov., with lac31 ( = CCUG 58604  = DSM 22620  = JCM 16156) as the type strain and A2 ( = CCUG 58212  = DSM 22619  = JCM 16157) as an additionally available reference strain. Also, based on our data, we propose emended descriptions of the genus and the species and .

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2011-04-01
2019-09-16
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Neighbour-joining tree based on partial 16S rRNA gene sequences showing the phylogenetic relationship of strains lac15, lac16, lac31 and A2 to other members of the bigeneric branch and the family , including all type species. [PDF](44 KB)

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Scanning electron micrographs of cells of strain lac31 . Bar, 2 µm (a), 1 µm (b) and 0.5 µm (c). Cells were grown on peptone-yeast extract-glucose (PYG) agar under anaerobic conditions for 48 h at 37 °C.

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Scanning electron micrographs of cells of strain A2. Bars, 5 µm (a), 2 µm (b) and 0.5 µm (c). Cells were grown on porcine gastric mucin (PGM) agar under anaerobic conditions for 14 days at 37 °C.

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Scanning electron micrographs of cells of strain lac31 . Bars, 2 µm (a) and 1 µm (b, c). Cells were grown on porcine jejunal mucus-mucosa (PJMM) agar under anaerobic conditions for 14 days at 37 °C.

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