1887

Abstract

Eight yellow-pigmented, Gram-negative, rod-shaped, oxidase-negative, motile, facultatively anaerobic bacteria were isolated from onion seed in South Africa and from an onion plant exhibiting centre rot symptoms in the USA. The isolates were assigned to the genus on the basis of phenotypic and biochemical tests. 16S rRNA gene sequence analysis and multilocus sequence analysis (MLSA), based on , , and sequences, confirmed the allocation of the isolates to the genus . MLSA further indicated that the isolates represented a novel species, which was phylogenetically most closely related to and Amplified fragment length polymorphism analysis also placed the isolates into a cluster separate from and . Compared with type strains of species of the genus that showed >97 % 16S rRNA gene sequence similarity with strain BD 390, the isolates exhibited 11–55 % whole-genome DNA–DNA relatedness, which confirmed the classification of the isolates in a novel species. The most useful phenotypic characteristics for the differentiation of the isolates from their closest phylogenetic neighbours are production of acid from amygdalin and utilization of adonitol and sorbitol. A novel species, sp. nov., is proposed, with type strain BD 390 ( = LMG 24248).

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2011-04-01
2019-12-06
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Supplements

vol. , part 4, pp. 932 - 937

Strains used in this study.

DNA–DNA relatedness between strains of sp. nov. and type strains of species of the genus that show >97 % 16S rRNA gene sequence similarity to strain BD 390 .

UPGMA dendrogram based on fluorescent amplified fragment polymorphism analysis of selected strains of species of the genus using the selective primer combination Eco-C/Mse-GC.

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