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Volume 61, Issue 4

sp. nov., isolated from an oyster Free

Abstract

A novel Gram-stain-positive, slightly halophilic, facultatively alkaliphilic, catalase-positive, oxidase-negative, endospore-forming, motile, rod-shaped, aerobic bacterium, designated strain JSM 082006, was isolated from an oyster collected from Naozhou Island in the South China Sea. The isolate grew in 0–18 % (w/v) NaCl (optimum, 0.5–4.0 %), at pH 6.0–10.5 (optimum, pH 8.0) and at 15–45 °C (optimum, 30 °C). -Diaminopimelic acid was present in the cell-wall peptidoglycan. The major cellular fatty acids were anteiso-C, iso-C and C. Strain JSM 082006 contained MK-7 as the predominant respiratory quinone and diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine as the major polar lipids. The genomic DNA G+C content was 40.2 mol%. Phylogenetic analysis based on 16S rRNA gene sequence comparisons revealed that strain JSM 082006 should be assigned to the genus and that it was most closely related to the type strains of (sequence similarity 99.1 %) and (97.5 %), followed by those of (96.6 %) and (96.1 %). Phylogenetic analysis, DNA–DNA relatedness values, phenotypic characteristics and chemotaxonomic data support the view that strain JSM 082006 represents a novel species of the genus , for which the name sp. nov. is proposed; the type strain is JSM 082006 ( = DSM 23009  = KCTC 13712).

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Funding
This study was supported by the:
  • National Basic Research Program of China (Award 2010CB833800)
  • National Natural Science Foundation of China (Award 30970007)
  • International Cooperation Research Program of Yunnan Province (Award 2009AC017)
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Bacillus nanhaiensis sp. nov., isolated from an oyster
Int J Syst Evol Microbiol 61, 888 (2011); https://doi.org/10.1099/ijs.0.022889-0
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