1887

Abstract

A novel actinomycete, designated strain RM287, was isolated from surface-sterilized roots of (Jacq.) Merr., collected from Bangkok, Thailand. The status of the novel strain was determined using a polyphasic taxonomic approach. Phylogenetic analyses based on 16S rRNA gene sequences showed that the organism formed a distinct phyletic line within the radiation of the genus . The 16S rRNA gene sequence similarity indicated that strain RM287 was most closely related to IMSNU 20056 (97.4 %), DSM 46095 (97.2 %), NRRL B-24129 (97.2 %), DSM 44654 (97 %) and DSM 44671 (97 %). The novel organism was found to have chemical properties typical of members of the genus such as -diaminopimelic acid as the dignostic diamino acid in the cell-wall peptidoglycan and arabinose and galactose as the diagnostic sugars. The major menaquinone was MK-9(H). The major fatty acids were iso-C iso-C, iso 2-OH-C and iso-C. The DNA G+C content was 71.7 mol%. Phenotypic data clearly distinguished the novel isolate from its closest relatives. The combined genotypic and phenotypic data indicated that strain RM287 represented a novel species of the genus . The proposed name for this organism is sp. nov., with the type strain RM287 ( = TISTR 1919 = BCC 35842 = NBRC 106095).

Funding
This study was supported by the:
  • , Kasetsart University Research and Development Institute
  • , Kitasato University
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.022699-0
2011-04-01
2020-08-12
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/61/4/951.html?itemId=/content/journal/ijsem/10.1099/ijs.0.022699-0&mimeType=html&fmt=ahah

References

  1. Becker B., Lechevalier M. P., Lechevalier H. A. 1965; Chemical composition of cell-wall preparations from strains of various form-genera of aerobic actinomycetes. Appl Microbiol 13:236–243[PubMed]
    [Google Scholar]
  2. Bian J., Li Y., Wang J., Song F.-H., Liu M., Dai H.-Q., Ren B., Gao H., Hu X. et al. 2009; Amycolatopsis marina sp. nov., an actinomycete isolated from an ocean sediment. Int J Syst Evol Microbiol 59:477–481 [CrossRef][PubMed]
    [Google Scholar]
  3. Collins M. D., Pirouz T., Goodfellow M., Minnikin D. E. 1977; Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 100:221–230[PubMed] [CrossRef]
    [Google Scholar]
  4. Duangmal K., Ward A. C., Goodfellow M. 2005; Selective isolation of members of the Streptomyces violaceoruber clade from soil. FEMS Microbiol Lett 245:321–327 [CrossRef][PubMed]
    [Google Scholar]
  5. Felsenstein J. 1985; Confidence limits on phylogeny: an appropriate use of the bootstrap. Evolution 39:783–791 [CrossRef]
    [Google Scholar]
  6. Felsenstein, J. (1993). phylip (phylogenetic inference package) version 3.5c. Distributed by the author. University of Washington, Seattle, USA.
  7. Fitch W. M. 1971; Towards defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20:406–416 [CrossRef]
    [Google Scholar]
  8. Fitch W. M., Margoliash E. 1967; Construction of phylogenetic trees. Science 155:279–284 [CrossRef][PubMed]
    [Google Scholar]
  9. Goodfellow M., Kim S. B., Minnikin D. E., Whitehead D., Zhou Z. H., Mattinson-Rose A. D. 2001; Amycolatopsis sacchari sp. nov., a moderately thermophilic actinomycete isolated from vegetable matter. Int J Syst Evol Microbiol 51:187–193[PubMed]
    [Google Scholar]
  10. Gordon R. E., Mihm J. M. 1957; A comparative study of some strains received as nocardiae. J Bacteriol 73:15–27[PubMed]
    [Google Scholar]
  11. Gordon R. E., Barnett D. A., Handerhan J. E., Pang C. H.-N. 1974; Nocardia coeliaca, Nocardia autotrophica, and the nocardin strain. Int J Syst Bacteriol 24:54–63 [CrossRef]
    [Google Scholar]
  12. Hasegawa T., Takizawa M., Tanida S. 1983; A rapid analysis for chemical grouping of aerobic actinomycetes. J Gen Appl Microbiol 29:319–322 [CrossRef]
    [Google Scholar]
  13. Huang Y., Paściak M., Liu Z. H., Xie Q., Gamian A. 2004; Amycolatopsis palatopharyngis sp. nov., a potentially pathogenic actinomycete isolated from a human clinical source. Int J Syst Evol Microbiol 54:359–363 [CrossRef][PubMed]
    [Google Scholar]
  14. Jacobson E., Grauville W. C., Fogs C. E. 1958 Color Harmony Manual, 4th edn. Chicago: Container Corporation of America;
    [Google Scholar]
  15. Kieser T., Bibb M. J., Buttner M. J., Chater K. F., Hopwood D. A. 2000 Practical Streptomyces Genetics Norwich: John Innes Foundation;
    [Google Scholar]
  16. Küster E., Williams S. T. 1964; Selection of media for isolation of streptomycetes. Nature 202:928–929[PubMed] [CrossRef]
    [Google Scholar]
  17. Labeda D. P., Donahue J. M., Williams N. M., Sells S. F., Henton M. M. 2003; Amycolatopsis kentuckyensis sp. nov., Amycolatopsis lexingtonensis sp. nov. and Amycolatopsis pretoriensis sp. nov., isolated from equine placentas. Int J Syst Evol Microbiol 53:1601–1605 [CrossRef][PubMed]
    [Google Scholar]
  18. Lane D. J. 1991; 16S/23S rRNA sequencing. In Nucleic Acid Techniques in Bacterial Systematics pp. 115–148 Edited by Stackebrandt E., Goodfellow M. Chichester: John Wiley & Sons;
    [Google Scholar]
  19. Lechevalier M. P., Prauser H., Labeda D. P., Ruan J.-S. 1986; Two new genera of nocardioform actinomycetes: Amycolata gen. nov. and Amycolatopsis gen. nov.. Int J Syst Bacteriol 36:29–37 [CrossRef]
    [Google Scholar]
  20. Lee S. D. 2006; Amycolatopsis jejuensis sp. nov. and Amycolatopsis halotolerans sp. nov., novel actinomycetes isolated from a natural cave. Int J Syst Evol Microbiol 56:549–553 [CrossRef][PubMed]
    [Google Scholar]
  21. Lee S. D. 2009; Amycolatopsis ultiminotia sp. nov., isolated from rhizosphere soil, and emended description of the genus Amycolatopsis . Int J Syst Evol Microbiol 59:1401–1404 [CrossRef][PubMed]
    [Google Scholar]
  22. Minnikin D. E., Patel P. V., Alshamaony L., Goodfellow M. 1977; Polar lipid composition in the classification of Nocardia and related bacteria. Int J Syst Bacteriol 27:104–117 [CrossRef]
    [Google Scholar]
  23. Page R. D. M. 1996; TreeView: an application to display phylogenetic trees on personal computers. Comput Appl Biosci 12:357–358[PubMed]
    [Google Scholar]
  24. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425[PubMed]
    [Google Scholar]
  25. Shirling E. B., Gottlieb D. 1966; Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16:313–340 [CrossRef]
    [Google Scholar]
  26. Tamaoka J., Komagata K. 1984; Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128 [CrossRef]
    [Google Scholar]
  27. Tan G. Y. A., Robinson S., Lacey E., Goodfellow M. 2006; Amycolatopsis australiensis sp. nov., an actinomycete isolated from arid soils. Int J Syst Evol Microbiol 56:2297–2301 [CrossRef][PubMed]
    [Google Scholar]
  28. Tan G. Y. A., Robinson S., Lacey E., Brown R., Kim W., Goodfellow M. 2007; Amycolatopsis regifaucium sp. nov., a novel actinomycete that produces kigamicins. Int J Syst Evol Microbiol 57:2562–2567 [CrossRef][PubMed]
    [Google Scholar]
  29. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [CrossRef][PubMed]
    [Google Scholar]
  30. Tomiyasu I. 1982; Mycolic acid composition and thermally adaptative changes in Nocardia asteroides . J Bacteriol 151:828–837[PubMed]
    [Google Scholar]
  31. Tseng M., Yang S.-F., Li W.-J., Jiang C.-L. 2006; Amycolatopsis taiwanensis sp. nov., from soil. Int J Syst Evol Microbiol 56:1811–1815 [CrossRef][PubMed]
    [Google Scholar]
  32. Uchida K., Aida K. 1984; An improved method for the glycolate test for simple identification of the acyl type of bacterial cell walls. J Gen Appl Microbiol 30:131–134 [CrossRef]
    [Google Scholar]
  33. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. et al. 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on the reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.022699-0
Loading
/content/journal/ijsem/10.1099/ijs.0.022699-0
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error