1887

Abstract

A novel actinomycete, designated strain RM287, was isolated from surface-sterilized roots of (Jacq.) Merr., collected from Bangkok, Thailand. The status of the novel strain was determined using a polyphasic taxonomic approach. Phylogenetic analyses based on 16S rRNA gene sequences showed that the organism formed a distinct phyletic line within the radiation of the genus . The 16S rRNA gene sequence similarity indicated that strain RM287 was most closely related to IMSNU 20056 (97.4 %), DSM 46095 (97.2 %), NRRL B-24129 (97.2 %), DSM 44654 (97 %) and DSM 44671 (97 %). The novel organism was found to have chemical properties typical of members of the genus such as -diaminopimelic acid as the dignostic diamino acid in the cell-wall peptidoglycan and arabinose and galactose as the diagnostic sugars. The major menaquinone was MK-9(H). The major fatty acids were iso-C iso-C, iso 2-OH-C and iso-C. The DNA G+C content was 71.7 mol%. Phenotypic data clearly distinguished the novel isolate from its closest relatives. The combined genotypic and phenotypic data indicated that strain RM287 represented a novel species of the genus . The proposed name for this organism is sp. nov., with the type strain RM287 ( = TISTR 1919 = BCC 35842 = NBRC 106095).

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2011-04-01
2019-10-20
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