1887

Abstract

A taxonomic study was carried out on a novel strain, designated CK-I3-6, which was isolated from deep-sea sediment of the south-west Indian Ocean Ridge. Cells were Gram-reaction-negative, oxidase- and catalase-positive, rod-shaped and non-motile. Growth was observed at 4–38 °C and in 1–12 % (w/v) NaCl. Cells were able to degrade gelatin and oxidize thiosulfate but did not reduce nitrate. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain CK-I3-6 belonged to the genus with a sequence similarity of 97.3 % to CHLG 1, while similarities with other taxa were <95.7 %. DNA–DNA hybridization showed that strain CK-I3-6 and CHLG 1 showed a low DNA–DNA relatedness (48±3 %). The principal fatty acids were C (7.8 %), Cω7 (66.6 %), summed feature 3 (Cω6 and/or Cω7; 6.3 %) and Cω8 cyclo (10.0 %). The chromosomal DNA G+C content was 67.5 mol%. On the basis of the combined genotypic and phenotypic data, strain CK-I3-6 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is CK-I3-6 ( = CCTCC AB 209064  = LMG 25230  = MCCC 1A03060).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.022376-0
2011-04-01
2019-10-24
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/61/4/728.html?itemId=/content/journal/ijsem/10.1099/ijs.0.022376-0&mimeType=html&fmt=ahah

References

  1. Ausubel F. M., Brent R., Kingston R. E., Moore D. D., Seidman J. G., Smith J. A., Struhl K.. (editors) ( 1995;). Short Protocols in Molecular Biology: a Compendium of Methods from Current Protocols in Molecular Biology, , 3rd edn.. New York:: Wiley;.
    [Google Scholar]
  2. Chen S., Shao Z.. ( 2009;). Isolation and diversity analysis of arsenite-resistant bacteria in communities enriched from deep-sea sediments of the Southwest Indian Ocean Ridge. . Extremophiles 13:, 39–48. [CrossRef][PubMed]
    [Google Scholar]
  3. Choi D. H., Cho B. C.. ( 2006;). Citreimonas salinaria gen. nov., sp. nov., a member of the Roseobacter clade isolated from a solar saltern. . Int J Syst Evol Microbiol 56:, 2799–2803 [CrossRef][PubMed].
    [Google Scholar]
  4. Lai Q., Yuan J., Wang B., Sun F., Qiao N., Zheng T., Shao Z.. ( 2009;). Bowmanella pacifica sp. nov., isolated from a pyrene-degrading consortium. . Int J Syst Evol Microbiol 59:, 1579–1582. [CrossRef][PubMed]
    [Google Scholar]
  5. Liu C., Shao Z.. ( 2005;). Alcanivorax dieselolei sp. nov., a novel alkane-degrading bacterium isolated from sea water and deep-sea sediment. . Int J Syst Evol Microbiol 55:, 1181–1186. [CrossRef][PubMed]
    [Google Scholar]
  6. Mesbah M., Whitman W. B.. ( 1989;). Measurement of deoxyguanosine/thymidine ratios in complex mixtures by high-performance liquid chromatography for determination of the mole percentage guanine+cytosine of DNA. . J Chromatogr A 479:, 297–306. [CrossRef][PubMed]
    [Google Scholar]
  7. Rzhetsky A., Nei M.. ( 1992;). A simple method for estimating and testing minimum-evolution trees. . Mol Biol Evol 9:, 945–967.
    [Google Scholar]
  8. Rzhetsky A., Nei M.. ( 1993;). Theoretical foundation of the minimum-evolution method of phylogenetic inference. . Mol Biol Evol 10:, 1073–1095.[PubMed]
    [Google Scholar]
  9. Saitou N., Nei M.. ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  10. Sasser M.. ( 1990;). Identification of bacteria by gas chromatography of cellular fatty acids. MIDI Technical Note 101. Newark, DE:: MIDI;.
    [Google Scholar]
  11. Sorokin D. Y., Tourova T. P., Muyzer G.. ( 2005;). Citreicella thiooxidans gen. nov., sp. nov., a novel lithoheterotrophic sulfur-oxidizing bacterium from the Black Sea. . Syst Appl Microbiol 28:, 679–687. [CrossRef][PubMed]
    [Google Scholar]
  12. Tamura K., Dudley J., Nei M., Kumar S.. ( 2007;). mega4: molecular evolutionary genetics analysis (mega) software version 4.0. . Mol Biol Evol 24:, 1596–1599 [CrossRef][PubMed].
    [Google Scholar]
  13. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. et al. ( 1987;). Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37:, 463–464. [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.022376-0
Loading
/content/journal/ijsem/10.1099/ijs.0.022376-0
Loading

Data & Media loading...

Supplements

Transmission electron micrograph of a negatively stained cell of strain CK-I3-6 grown on Marine Agar for 24 h at 28 °C.

IMAGE

Cellular fatty acid contents of strain CK-I3-6 and CHLG 1 . [PDF](31.1KB)

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error