1887

Abstract

A novel psychrotolerant bacterial strain, BCw111, was isolated from seawater samples from the Chukchi Sea in the Arctic Ocean. Cells of strain BCw111 were Gram-negative, motile, facultatively anaerobic, curved rods and were able to grow at 0–30 °C (optimum 23–25 °C). Strain BCw111 had Q-8 as the major respiratory quinone and contained iso-C 2-OH and/or Cω7 (28.13 %), C (13.28 %) and C (12.90 %) as the major cellular fatty acids. The genomic DNA G+C content was 41.3 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain BCw11 formed a distinct lineage within the genus and exhibited the highest 16S rRNA gene sequence similarity with 537 (97.8 %) and SMK-10 (97.1 %). Based on phenotypic characteristics, phylogenetic analysis and DNA–DNA relatedness, a novel species, sp. nov., is proposed. The type strain is BCw111 ( = CGMCC 1.9127  = LMG 25329  = DSM 22576).

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2011-04-01
2019-09-16
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References

  1. Bowman J. P. , Gosink J. J. , McCammon S. A. , Lewis T. E. , Nichols D. S. , Nichols P. D. , Skerratt J. H. , Staley J. T. , McMeekin T. A. . ( 1998; ). Colwellia demingiae sp. nov., Colwellia hornerae sp. nov., Colwellia rossensis sp. nov. and Colwellia psychrotropica sp. nov.: psychrophilic Antarctic species with the ability to synthesize docosahexaenoic acid (22 : 6ω3). . Int J Syst Bacteriol 48:, 1171–1180. [CrossRef]
    [Google Scholar]
  2. Cashion P. , Holder-Franklin M. A. , McCully J. , Franklin M. . ( 1977; ). A rapid method for the base ratio determination of bacterial DNA. . Anal Biochem 81:, 461–466. [CrossRef] [PubMed]
    [Google Scholar]
  3. Choi E. J. , Kwon H. C. , Koh H. Y. , Kim Y. S. , Yang H. O. . ( 2010; ). Colwellia asteriadis sp. nov., a marine bacterium isolated from the starfish Asterias amurensis . . Int J Syst Evol Microbiol 60:, 1952–1957. [CrossRef] [PubMed]
    [Google Scholar]
  4. Chun J. , Lee J.-H. , Jung Y. , Kim M. , Kim S. , Kim B. K. , Lim Y.-W. . ( 2007; ). EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. . Int J Syst Evol Microbiol 57:, 2259–2261. [CrossRef] [PubMed]
    [Google Scholar]
  5. Collins M. D. . ( 1985; ). Isoprenoid quinone analysis in classification and identification. . In Chemical Methods in Bacterial Systematics, pp. 267–287. Edited by Goodfellow M. , Minnikin D. E. . . London:: Academic Press;.
    [Google Scholar]
  6. De Ley J. , Cattoir H. , Reynaerts A. . ( 1970; ). The quantitative measurement of DNA hybridization from renaturation rates. . Eur J Biochem 12:, 133–142. [CrossRef] [PubMed]
    [Google Scholar]
  7. Deming J. W. , Somers L. K. , Straube W. L. , Swartz D. G. , MacDonell M. T. . ( 1988; ). Isolation of an obligately barophilic bacterium and description of a new genus, Colwellia gen. nov.. Syst Appl Microbiol 10:, 152–160.[CrossRef]
    [Google Scholar]
  8. Dong X.-Z. , Cai M.-Y. . ( 2001; ). Manual for the Systematic Identification of General Bacteria, pp. 370–398. Beijing:: Science Press; (in Chinese).
    [Google Scholar]
  9. Felsenstein, J. (2009). phylip (phylogeny inference package) version 3.69. Distributed by the author. Department of Genome Sciences, University of Washington, Seattle, USA.
  10. Hall T. A. . ( 1999; ). BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. . Nucleic Acids Symp Ser 41:, 95–98.
    [Google Scholar]
  11. Huß V. A. R. , Festl H. , Schleifer K. H. . ( 1983; ). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. . Syst Appl Microbiol 4:, 184–192.[CrossRef]
    [Google Scholar]
  12. Jung S.-Y. , Oh T.-K. , Yoon J.-H. . ( 2006; ). Colwellia aestuarii sp. nov., isolated from a tidal flat sediment in Korea. . Int J Syst Evol Microbiol 56:, 33–37. [CrossRef] [PubMed]
    [Google Scholar]
  13. Mesbah M. , Premachandran U. , Whitman W. B. . ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. . Int J Syst Bacteriol 39:, 159–167. [CrossRef]
    [Google Scholar]
  14. Nogi Y. , Hosoya S. , Kato C. , Horikoshi K. . ( 2004; ). Colwellia piezophila sp. nov., a novel piezophilic species from deep-sea sediments of the Japan Trench. . Int J Syst Evol Microbiol 54:, 1627–1631. [CrossRef] [PubMed]
    [Google Scholar]
  15. Sasser, M. (1990). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc.
  16. Smibert R. M. , Krieg N. R. . ( 1994; ). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by Gerhardt P. , Murray R. G. E. , Wood W. A. , Krieg N. R. . . Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  17. Stackebrandt E. , Goebel B. M. . ( 1994; ). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. . Int J Syst Bacteriol 44:, 846–849. [CrossRef]
    [Google Scholar]
  18. Tamura K. , Dudley J. , Nei M. , Kumar S. . ( 2007; ). mega4: molecular evolutionary genetics analysis (mega) software version 4.0. . Mol Biol Evol 24:, 1596–1599. [CrossRef] [PubMed]
    [Google Scholar]
  19. Thompson J. D. , Gibson T. J. , Plewniak F. , Jeanmougin F. , Higgins D. G. . ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25:, 4876–4882. [CrossRef] [PubMed]
    [Google Scholar]
  20. Wayne L. G. , Brenner D. J. , Colwell R. R. , Grimont P. A. D. , Kandler O. , Krichevsky M. I. , Moore L. H. , Moore W. E. C. , Murray R. G. E. et al. ( 1987; ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37:, 463–464. [CrossRef]
    [Google Scholar]
  21. Weisburg W. G. , Barns S. M. , Pelletier D. A. , Lane D. J. . ( 1991; ). 16S ribosomal DNA amplification for phylogenetic study. . J Bacteriol 173:, 697–703.[PubMed]
    [Google Scholar]
  22. Wu C. , Lu X. , Qin M. , Wang Y. , Ruan J. . ( 1989;). Analysis of menaquinone compound in microbial cells by HPLC. . Microbiology [English translation of Microbiology (Beijing)] 16:, 176–178.
    [Google Scholar]
  23. Yumoto I. , Kawasaki K. , Iwata H. , Matsuyama H. , Okuyama H. . ( 1998; ). Assignment of Vibrio sp. strain ABE-1 to Colwellia maris sp. nov., a new psychrophilic bacterium. . Int J Syst Bacteriol 48:, 1357–1362. [CrossRef] [PubMed]
    [Google Scholar]
  24. Zhang D.-C. , Yu Y. , Xin Y.-H. , Liu H.-C. , Zhou P.-J. , Zhou Y.-G. . ( 2008; ). Colwellia polaris sp. nov., a psychrotolerant bacterium isolated from Arctic sea ice. . Int J Syst Evol Microbiol 58:, 1931–1934. [CrossRef] [PubMed]
    [Google Scholar]
  25. Zhang L. , Wang Y. , Dai J. , Tang Y. , Yang Q. , Luo X. , Fang C. X. . ( 2009; ). Bacillus korlensis sp. nov., a moderately halotolerant bacterium isolated from a sand soil sample in China. . Int J Syst Evol Microbiol 59:, 1787–1792. [CrossRef] [PubMed]
    [Google Scholar]
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vol. , part 4, pp. 850 - 853

Cellular fatty acid compositions of sp. nov. BCw111 and its closest phylogenetic neighbours.

Maximum-parsimony (Fig. S1) and maximum-likelihood (Fig. S2) trees based on 16S rRNA gene sequences showing the phylogenetic relationships of sp. nov. BCw111 and related taxa.

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