1887

Abstract

Two red-pigmented, strictly aerobic, pleomorphic rod-shaped and extremely halophilic archaea, designated strains HST01-2R and HST03, were isolated from salt in a fish sauce sample from Thailand. The novel strains grew optimally at 37 °C, pH 7.0, and in the presence of 20–25 % (w/v) NaCl. The DNA G+C contents of the isolates were 61.6–62.2 mol%. Phylogenetic analysis based on a comparison of 16S rRNA gene sequences revealed that strains HST01-2R and HST03 were placed in the radiation of species of the genus . The chemotaxonomic properties of the two strains, i.e. the presence of MK-8 and MK-8(H) as the major menaquinone components and CC derivatives of phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, phosphatidylglycerol sulfate and a triglycosyl diether as major polar lipids, supported the assignment of the two strains to the genus . Nevertheless, several phenotypic features and the low DNA–DNA relatedness between the two strains and related species of the genus (13.4–46.9 %) enabled the strains to be distinguished from each other and from recognized species. Therefore, strains HST01-2R and HST03 represent two novel species in the genus , for which the names sp. nov. and sp. nov. are proposed, respectively. The type strains are HST01-2R (=BCC 40029=JCM 15759=PCU 313) and HST03 (=BCC 40030=JCM 15760=PCU 314).

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2011-02-01
2021-04-18
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