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Abstract

A nitrogen-fixing bacterium, designated strain S27, was isolated from rhizosphere soil of . Phylogenetic analysis based on a fragment of the gene and the full-length 16S rRNA gene sequence revealed that strain S27 is a member of the genus . High levels of 16S rRNA gene sequence similarity were found between strain S27 and DSM 1735 (97.3 %), DSM 17841 (96.9 %), DSM 17842 (96.7 %) and DSM 18202 (96.6 %). However, DNA–DNA hybridization values between strain S27 and the four type strains were 37.64 %, 23.12 %, 25.6 % and 34.99 %, respectively. Levels of 16S rRNA gene sequence similarity between strain S27 and the type strains of other recognized members of the genus were below 96.5 %. The DNA G+C content of strain S27 was 46.0 mol%. The major fatty acids were anteiso-C, C and iso-C. The major isoprenoid quinone was MK-7. On the basis of its phenotypic characteristics and DNA–DNA hybridization results, strain S27 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is S27 ( = CGMCC 1.10238  = DSM 23020).

Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 30670036)
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2011-04-01
2021-05-18
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