1887

Abstract

A nitrogen-fixing bacterium, designated strain S27, was isolated from rhizosphere soil of . Phylogenetic analysis based on a fragment of the gene and the full-length 16S rRNA gene sequence revealed that strain S27 is a member of the genus . High levels of 16S rRNA gene sequence similarity were found between strain S27 and DSM 1735 (97.3 %), DSM 17841 (96.9 %), DSM 17842 (96.7 %) and DSM 18202 (96.6 %). However, DNA–DNA hybridization values between strain S27 and the four type strains were 37.64 %, 23.12 %, 25.6 % and 34.99 %, respectively. Levels of 16S rRNA gene sequence similarity between strain S27 and the type strains of other recognized members of the genus were below 96.5 %. The DNA G+C content of strain S27 was 46.0 mol%. The major fatty acids were anteiso-C, C and iso-C. The major isoprenoid quinone was MK-7. On the basis of its phenotypic characteristics and DNA–DNA hybridization results, strain S27 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is S27 ( = CGMCC 1.10238  = DSM 23020).

Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 30670036)
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2011-04-01
2024-04-26
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References

  1. Ash C., Priest F. G., Collins M. D. 1993; Molecular identification of rRNA group 3 bacilli (Ash, Farrow, Wallbanks and Collins) using a PCR probe test. Proposal for the creation of a new genus Paenibacillus . Antonie van Leeuwenhoek 64:253–260 [View Article][PubMed]
    [Google Scholar]
  2. Beneduzi A., Costa P. B., Parma M., Melo I. S., Bodanese-Zanettini M. H., Passaglia L. M. 2010; Paenibacillus riograndensis sp. nov., a nitrogen-fixing species isolated from the rhizosphere of Triticum aestivum . Int J Syst Evol Microbiol 60:128–133 [View Article][PubMed]
    [Google Scholar]
  3. Berge O., Guinebretière M. H., Achouak W., Normand P., Heulin T. 2002; Paenibacillus graminis sp. nov. and Paenibacillus odorifer sp. nov., isolated from plant roots, soil and food. Int J Syst Evol Microbiol 52:607–616[PubMed]
    [Google Scholar]
  4. Coelho M. R., Carneiro N. P., Marriel I. E., Seldin L. 2009; Molecular detection of nifH gene-containing Paenibacillus in the rhizosphere of sorghum (Sorghum bicolor) sown in Cerrado soil. Lett Appl Microbiol 48:611–617 [View Article][PubMed]
    [Google Scholar]
  5. Collins M. D. 1985; Analysis of isoprenoid quinones. Methods Microbiol 18:329–366 [View Article]
    [Google Scholar]
  6. Collins M. D., Goodfellow M., Minnikin D. E. 1980; Fatty acid, isoprenoid quinone and polar lipid composition in the classification of Curtobacterium and related taxa. J Gen Microbiol 118:29–37[PubMed]
    [Google Scholar]
  7. Cowan S. T., Steel K. J. 1965 Manual for the Identification of Medical Bacteria London: Cambridge University Press;
    [Google Scholar]
  8. Ding Y., Wang J., Liu Y., Chen S. 2005; Isolation and identification of nitrogen-fixing bacilli from plant rhizospheres in Beijing region. J Appl Microbiol 99:1271–1281 [View Article][PubMed]
    [Google Scholar]
  9. Elo S., Suominen I., Kämpfer P., Juhanoja J., Salkinoja-Salonen M., Haahtela K. 2001; Paenibacillus borealis sp. nov., a nitrogen-fixing species isolated from spruce forest humus in Finland. Int J Syst Evol Microbiol 51:535–545[PubMed]
    [Google Scholar]
  10. Gordon R. E., Haynes W. C., Pang C. H.-N. 1973 The Genus Bacillus. US Department of Agriculture Handbook no. 427 Washington, DC: Agricultural Research Service;
    [Google Scholar]
  11. Hong Y. Y., Ma Y. C., Zhou Y. G., Gao F., Liu H. C., Chen S. F. 2009; Paenibacillus sonchi sp. nov., a nitrogen-fixing species isolated from the rhizosphere of Sonchus oleraceus . Int J Syst Evol Microbiol 59:2656–2661 [View Article][PubMed]
    [Google Scholar]
  12. Komagata K., Suzuki K. 1987; Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19:161–207 [View Article]
    [Google Scholar]
  13. Kumar S., Tamura K., Nei M. 2004; mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5:150–163 [View Article][PubMed]
    [Google Scholar]
  14. Ma Y., Xia Z., Liu X., Chen S. 2007a; Paenibacillus sabinae sp. nov., a nitrogen-fixing species isolated from the rhizosphere soils of shrubs. Int J Syst Evol Microbiol 57:6–11 [View Article][PubMed]
    [Google Scholar]
  15. Ma Y., Zhang J., Chen S. 2007b; Paenibacillus zanthoxyli sp. nov., a novel nitrogen-fixing species isolated from the rhizosphere of Zanthoxylum simulans . Int J Syst Evol Microbiol 57:873–877 [View Article][PubMed]
    [Google Scholar]
  16. Minnikin D. E., Collins M. D., Goodfellow M. 1979; Fatty acid and polar lipid composition in the classification of Cellulomonas, Oerskovia and related taxa. J Appl Bacteriol 47:87–95 [CrossRef]
    [Google Scholar]
  17. Priest F. G., Goodfellow M., Todd C. 1981; The genus Bacillus: a numerical analysis. In The Aerobic Endospore-Forming Bacteria. Classification and Identification pp. 91–103 Edited by Berkeley R. C. W., Goodfellow M. London: Academic Press;
    [Google Scholar]
  18. Rhodes-Roberts M. 1981; The taxonomy of some nitrogen-fixing Bacillus species with special reference to nitrogen fixation. In The Aerobic Endospore-Forming Bacteria. Classification and Identification pp. 315–335 Edited by Berkeley R. C. W., Goodfellow M. London: Academic Press;
    [Google Scholar]
  19. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: a Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press;
    [Google Scholar]
  20. Sasser M., Kunitsky C., Jackoway G., Ezzell J. W., Teska J. D., Harper B., Parker S., Barden D., Blair H. et al. 2005; Identification of Bacillus anthracis from culture using gas chromatographic analysis of fatty acid methyl esters. J AOAC Int 88:178–181[PubMed]
    [Google Scholar]
  21. Shida O., Takagi H., Kadowaki K., Komagata K. 1996; Proposal for two new genera, Brevibacillus gen. nov. and Aneurinibacillus gen. nov. Int J Syst Bacteriol 46:939–946 [View Article][PubMed]
    [Google Scholar]
  22. Shida O., Takagi H., Kadowaki K., Nakamura L. K., Komagata K. 1997; Transfer of Bacillus alginolyticus, Bacillus chondroitinus, Bacillus curdlanolyticus, Bacillus glucanolyticus, Bacillus kobensis and Bacillus thiaminolyticus to the genus Paenibacillus and emended description of the genus Paenibacillus . Int J Syst Bacteriol 47:289–298 [View Article][PubMed]
    [Google Scholar]
  23. Shirling E. B., Gottlieb D. 1966; Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16:313–340 [View Article]
    [Google Scholar]
  24. Staneck J. L., Roberts G. D. 1974; Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol 28:226–231[PubMed]
    [Google Scholar]
  25. Suominen I., Spröer C., Kämpfer P., Rainey F. A., Lounatmaa K., Salkinoja-Salonen M. 2003; Paenibacillus stellifer sp. nov., a cyclodextrin-producing species isolated from paperboard. Int J Syst Evol Microbiol 53:1369–1374 [View Article][PubMed]
    [Google Scholar]
  26. Tamaoka J., Komagata K. 1984; Determination of DNA base composition by reverse-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128 [View Article]
    [Google Scholar]
  27. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. et al. 1987; Report of the Ad-Hoc-Committee on Reconciliation of Approaches to Bacterial Systematics. Int J Syst Bacteriol 37:463–464 [View Article]
    [Google Scholar]
  28. Weaver P. F., Wall J. D., Gest H. 1975; Characterization of Rhodopseudomonas capsulata . Arch Microbiol 105:207–216 [View Article][PubMed]
    [Google Scholar]
  29. Yanagi M., Yamasato K. 1993; Phylogenetic analysis of the family Rhizobiaceae and related bacteria by sequencing of 16S rRNA gene using PCR and DNA sequencer. FEMS Microbiol Lett 107:115–120 [View Article][PubMed]
    [Google Scholar]
  30. Yoon J.-H., Kim H., Kim S.-B., Kim H.-J., Kim W. Y., Lee S. T., Goodfellow M., Park Y.-H. 1996; Identification of Saccharomonospora strains by the use of genomic DNA fragments and rRNA gene probes. Int J Syst Bacteriol 46:502–505 [View Article]
    [Google Scholar]
  31. Ziemke F., Höfle M. G., Lalucat J., Rosselló-Mora R. 1998; Reclassification of Shewanella putrefaciens Owen’s genomic group II as Shewanella baltica sp. nov. Int J Syst Bacteriol 48:179–186 [View Article][PubMed]
    [Google Scholar]
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