1887

Abstract

Two Gram-stain-positive, non-motile, non-spore-forming cocci (strains MK-7 and MPA-33) were isolated from poultry houses. Strain MK-7 was isolated on marine broth agar from coquina, a food supplement for female ducks used in a duck-fattening farm. Strain MPA-33 was isolated from the air of a turkey house on TSA after filter sampling. On the basis of 16S rRNA gene sequence similarity studies, both strains were shown to belong to the genus ; MK-7 was most closely related to YKJ-115 (99.3 % similarity) and MPA-33 was most closely related to YKJ-101 (98.8 %). The quinone system of MK-7 was composed of equal amounts of menaquinones MK-7 and MK-6 and that of MPA-33 contained 76 % MK-7 and 24 % MK-6. The polar lipid profile of strain MK-7 consisted of the major compounds diphosphatidylglycerol and phosphatidylglycerol and six unidentified lipids present in minor to moderate amounts. In strain MPA-33, diphosphatidylglycerol was the single predominant lipid, whereas phosphatidylglycerol was detected in moderate amounts. In addition, one unidentified phospholipid and four unidentified lipids were detected. Fatty acid profiles with iso-15 : 0 and anteiso-15 : 0 as major fatty acids supported the affiliation of the strains to the genus . The results of physiological and biochemical tests as well as DNA–DNA hybridizations allowed clear phenotypic differentiation of strains MK-7 and MPA-33 from the most closely related species. Strains MK-7 and MPA-33 therefore represent novel species, for which the names sp. nov. (type strain MK-7 =DSM 22419 =CCM 7682 =CCUG 57956) and sp. nov. (type strain MPA-33 =DSM 22420 =CCM 7679 =CCUG 57953) are proposed.

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2011-02-01
2019-10-23
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Phylogenetic analysis based on 16S rRNA gene sequences available from the EMBL database (accession numbers in parentheses). Tree building was performed using the maximum-likelihood method with fastDNAml and no conservation filter. [PDF](34 KB)

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Polar lipid profiles of strains MK-7 (a) and MPA-33 (b), DSM 17274 (c) and CCUG 42722 (d). DPG, Diphosphatidylglycerol; PG, phosphatidylglycerol; PL1, unidentified phospholipid; L1–L7, unidentified lipids not stainable with any of the spray reagents applied.

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