1887

Abstract

A motile bacterium, designated strain TDA-040725-5, was isolated from the haemolymph of a Chinese mitten crab, , with tremor disease. Based on 16S rRNA gene sequence analysis, the strain was phylogenetically distinct from other spiroplasmas but was closely related to ATCC 29335. Cells of strain TDA-040725-5 were variable in length and shape, helical and motile, as determined by phase-contrast light microscopy. Examination by electron microscopy revealed wall-less cells delimited by a single membrane. The strain grew in M1D or R-2 liquid media at 20–40 °C, with optimum growth at 30 °C. Doubling time at the optimal temperature was 24 h. The strain catabolized glucose and hydrolysed arginine but did not hydrolyse urea. The DNA G+C content was 29.7±1 mol%. The genome size was ~1.4–1.6 Mbp. Serological analysis, performed using the deformation test, did not reveal any reciprocal titres ≥320, indicating that strain TDA-040725-5 had minimal cross-reactivity to strains of recognized species of the genus . Based on this evidence, strain TDA-040725-5 ( = CCTCC M 207170  = DSM 21848) represents a novel species of the genus , for which the name sp. nov. is proposed, belonging to the novel serological group XLIII.

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2011-04-01
2019-09-22
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References

  1. Aluotto B. B., Wittler R. G., Williams C. O., Faber J. E.. ( 1970;). Standardized bacteriologic techniques for the characterization of Mycoplasma species. . Int J Syst Bacteriol 20:, 35–58. [CrossRef]
    [Google Scholar]
  2. Bi K., Huang H., Gu W., Wang J., Wang W.. ( 2008;). Phylogenetic analysis of spiroplasmas from three freshwater crustaceans (Eriocheir sinensis, Procambarus clarkia and Penaeus vannamei) in China. . J Invertebr Pathol 99:, 57–65. [CrossRef][PubMed]
    [Google Scholar]
  3. Brown D. R., Whitcomb R. F., Bradbury J. M.. ( 2007;). Revised minimal standards for description of new species of the class Mollicutes (division Tenericutes). . Int J Syst Evol Microbiol 57:, 2703–2719. [CrossRef][PubMed]
    [Google Scholar]
  4. Carle P., Laigret F., Tully J. G., Bové J. M.. ( 1995;). Heterogeneity of genome sizes within the genus Spiroplasma. . Int J Syst Bacteriol 45:, 178–181. [CrossRef][PubMed]
    [Google Scholar]
  5. Gasparich G. E., Whitcomb R. F., Dodge D., French F. E., Glass J., Williamson D. L.. ( 2004;). The genus Spiroplasma and its non-helical descendants: phylogenetic classification, correlation with phenotype and roots of the Mycoplasma mycoides clade. . Int J Syst Evol Microbiol 54:, 893–918. [CrossRef][PubMed]
    [Google Scholar]
  6. Moulder R. W., French F. E., Chang C. J.. ( 2002;). Simplified media for spiroplasmas associated with tabanid flies. . Can J Microbiol 48:, 1–6. [CrossRef][PubMed]
    [Google Scholar]
  7. Nunan L. M., Pantoja C. R., Salazar M., Aranguren F., Lightner D. V.. ( 2004;). Characterization and molecular methods for detection of a novel spiroplasma pathogenic to Penaeus vannamei. . Dis Aquat Organ 62:, 255–264. [CrossRef][PubMed]
    [Google Scholar]
  8. Nunan L. M., Lightner D. V., Oduori M. A., Gasparich G. E.. ( 2005;). Spiroplasma penaei sp. nov., associated with mortalities in Penaeus vannamei, Pacific white shrimp. . Int J Syst Evol Microbiol 55:, 2317–2322. [CrossRef][PubMed]
    [Google Scholar]
  9. Posada D., Crandall K. A.. ( 1998;). modeltest: testing the model of DNA substitution. . Bioinformatics 14:, 817–818. [CrossRef][PubMed]
    [Google Scholar]
  10. Regassa L. B., Gasparich G. E.. ( 2006;). Spiroplasmas: evolutionary relationships and biodiversity. . Front Biosci 11:, 2983–3002. [CrossRef][PubMed]
    [Google Scholar]
  11. Regassa L. B., Stewart K. M., Murphy A. C., French F. E., Lin T., Whitcomb R. F.. ( 2004;). Differentiation of group VIII Spiroplasma strains with sequences of the 16S–23S rDNA intergenic spacer region. . Can J Microbiol 50:, 1061–1067. [CrossRef][PubMed]
    [Google Scholar]
  12. Swofford, D. L. (2002). paup*: Phylogenetic analysis using parsimony (and other methods), version 4.0b10. Sunderland, MA: Sinauer Associates.
  13. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G.. ( 1997;). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25:, 4876–4882. [CrossRef][PubMed]
    [Google Scholar]
  14. Tully J. G.. ( 1983;). Cloning and filtration techniques for mycoplasmas. . Methods Mycoplasmol 1:, 173–177.
    [Google Scholar]
  15. Tully J. G., Whitcomb R. F., Williamson D. L., Clark H. F.. ( 1976;). Suckling mouse cataract agent is a helical wall-free prokaryote (spiroplasma) pathogenic for vertebrates. . Nature 259:, 117–120. [CrossRef][PubMed]
    [Google Scholar]
  16. Tully J. G., Whitcomb R. F., Clark H. F., Williamson D. L.. ( 1977;). Pathogenic mycoplasmas: cultivation and vertebrate pathogenicity of a new spiroplasma. . Science 195:, 892–894. [CrossRef][PubMed]
    [Google Scholar]
  17. Tully J. G., Whitcomb R. F., Rose D. L., Bove J. M.. ( 1982;). Spiroplasma mirum, a new species from the rabbit tick (Haemaphysalis leporispalustris). . Int J Syst Bacteriol 32:, 92–100. [CrossRef]
    [Google Scholar]
  18. Tully J. G., Whitcomb R. F., Rose D. L., Williamson D. L., Bové J. M.. ( 1983;). Characterization and taxonomic status of tick spiroplasmas: a review. . Yale J Biol Med 56:, 599–603.[PubMed]
    [Google Scholar]
  19. Wang W., Gu Z.. ( 2002;). Rickettsia-like organism associated with tremor disease and mortality of the Chinese mitten crab Eriocheir sinensis. . Dis Aquat Organ 48:, 149–153. [CrossRef][PubMed]
    [Google Scholar]
  20. Wang W., Zhu N., Gu Z., Du K., Xu Z.. ( 2002;). Study on the transmission of tremor disease (TD) in the Chinese mitten crab, Eriocheir sinensis (Crustacea: Decapoda). . J Invertebr Pathol 81:, 202–204. [CrossRef][PubMed]
    [Google Scholar]
  21. Wang W., Rong L., Gu W., Du K., Chen J.. ( 2003;). Study on experimental infections of Spiroplasma from the Chinese mitten crab in crayfish, mice and embryonated chickens. . Res Microbiol 154:, 677–680. [CrossRef][PubMed]
    [Google Scholar]
  22. Wang W., Chen J., Du K., Xu Z.. ( 2004;a). Morphology of spiroplasmas in the Chinese mitten crab Eriocheir sinensis associated with tremor disease. . Res Microbiol 155:, 630–635. [CrossRef][PubMed]
    [Google Scholar]
  23. Wang W., Wen B., Gasparich G. E., Zhu N., Rong L., Chen J., Xu Z.. ( 2004;b). A spiroplasma associated with tremor disease in the Chinese mitten crab (Eriocheir sinensis). . Microbiology 150:, 3035–3040. [CrossRef][PubMed]
    [Google Scholar]
  24. Wang W., Gu W., Ding Z., Ren Y., Chen J., Hou Y.. ( 2005;). A novel Spiroplasma pathogen causing systemic infection in the crayfish Procambarus clarkii (Crustacea: Decapod), in China. . FEMS Microbiol Lett 249:, 131–137. [CrossRef][PubMed]
    [Google Scholar]
  25. Wang J., Huang H., Feng Q., Liang T., Bi K., Gu W., Wang W., Shields J. D.. ( 2009;). Enzyme-linked immunosorbent assay for the detection of pathogenic spiroplasma in commercially exploited crustaceans from China. . Aquaculture 292:, 166–171. [CrossRef]
    [Google Scholar]
  26. Wei Z.. ( 1999;). [ An investigation on the epidemiology of tremor disease of the Chinese mitten crab, Eriocheir sinensis. ]. Freshw Fish 7:, 16–71 (in Chinese).
    [Google Scholar]
  27. Whitcomb R. F., Tully J. G., Rose D. L., Stephens E. B., Smith A., McCoy R. E., Barile M. F.. ( 1982;). Wall-less prokaryotes from fall flowers in central United States and Maryland. . Curr Microbiol 7:, 285–290. [CrossRef]
    [Google Scholar]
  28. Williamson D. L.. ( 1983;). Specialized electron microscopic techniques for spiroplasmas in plant and insect tissues. . Methods Mycoplasmol 1:, 71–76.
    [Google Scholar]
  29. Williamson D. L., Whitcomb R. F., Tully J. G.. ( 1978;). The spiroplasma deformation test, a new serological method. . Curr Microbiol 1:, 203–207. [CrossRef]
    [Google Scholar]
  30. Ye X., Chen Y.. ( 1991;). [ Isolation, cultivation, and nutrition characters of spiroplasmas from flowers. ]. J Nanjing Agricultural University 14:, 58–62 (in Chinese).
    [Google Scholar]
  31. Zbinden M., Cambon-Bonavita M. A.. ( 2003;). Occurrence of Deferribacterales and Entomoplasmatales in the deep-sea Alvinocarid shrimp Rimicaris exoculata gut. . FEMS Microbiol Ecol 46:, 23–30. [CrossRef][PubMed]
    [Google Scholar]
  32. Zhang F., Wang J., Zhu Q., Zhou G.. ( 2002;). [ Electron microscopy of pathologen of appendage shaking disease of Eriocheir sinensis. ]. J Dalian Fisheries University 17:, 336–340 (in Chinese).
    [Google Scholar]
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