1887

Abstract

A Gram-negative, aerobic, heterotrophic bacterium, designated KP1-19, was isolated from a 22-year-old volcanic deposit at a site lacking vegetation on the island of Miyake, Japan. Strain KP1-19 was able to use thiosulfate (optimum concentration 10 mM) as an additional energy source. 16S rRNA gene sequence analysis indicated that strain KP1-19 was closely related to CS-K2 within the class (97.7 % 16S rRNA gene sequence similarity). The cellular fatty acid profile was characteristic of the genus : the major fatty acids (>5 %) were C, C 7 and C 7 and minor amounts of C 3-OH were also found. DNA–DNA relatedness between strain KP1-19 and LMG 19593 was 18 %. Therefore, strain KP1-19 represents a novel species, for which the name sp. nov. is proposed. The type strain is KP1-19 (=LMG 24869 =NBRC 105857 =CIP 109929).

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2011-02-01
2020-08-13
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References

  1. Ezaki T., Hashimoto Y., Yabuuchi E. 1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229 [CrossRef]
    [Google Scholar]
  2. Ikemoto S., Kathoh K., Komagata K. 1978; Cellular fatty acid composition in methanol-utilizing bacteria. J Gen Appl Microbiol 24:41–49 [CrossRef]
    [Google Scholar]
  3. King G. M. 2007; Chemolithotrophic bacteria: distributions, functions and significance in volcanic environment. Microbes Environ 22:309–319 [CrossRef]
    [Google Scholar]
  4. King G. M., Weber C. F., Nanba K., Sato Y., Ohta H. 2008; Atmospheric CO and hydrogen uptake and CO oxidizer phylogeny for Miyake-jima, Japan volcanic deposits. Microbes Environ 23:299–305 [CrossRef]
    [Google Scholar]
  5. Komagata K., Suzuki K. 1987; Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19:161–207
    [Google Scholar]
  6. Lu H., Fujimura R., Sato Y., Nanba K., Kamijo T., Ohta H. 2008; Characterization of Herbaspirillum - and Limnobacter -related strains isolated from young volcanic deposits in Miyake-jima Island, Japan. Microbes Environ 23:66–72 [CrossRef]
    [Google Scholar]
  7. Ohta H., Hattori T. 1983; Agromonas oligotrophica gen. nov., sp. nov. a nitrogen-fixing oligotrophic bacterium. Antonie van Leeuwenhoek 49:429–446
    [Google Scholar]
  8. Ohta H., Ogiwara K., Murakami E., Takahashi H., Sekiguchi M., Koshida K., Someya T., Morishima W., Rondal J. D. other authors 2003; Quinone profiling of bacterial populations developed in the surface layer of volcanic mudflow deposits from Mt. Pinatubo (the Philippines). Soil Biol Biochem 35:1155–1158 [CrossRef]
    [Google Scholar]
  9. Ohta H., Hattori R., Ushiba Y., Mitsui H., Ito M., Watanabe H., Tonosaki A., Hattori T. 2004; Sphingomonas oligophenolica sp. nov., a halo- and organo-sensitive oligotrophic bacterium from paddy soil that degrades phenolic acids at low concentrations. Int J Syst Evol Microbiol 54:2185–2190 [CrossRef]
    [Google Scholar]
  10. Ostle A. G., Holt J. G. 1982; Nile blue A as a fluorescent stain for poly-beta-hydroxybutyrate. Appl Environ Microbiol 44:238–241
    [Google Scholar]
  11. Page R. D. M. 1996; TreeView: an application to display phylogenetic trees on personal computers. Comput Appl Biosci 12:357–358
    [Google Scholar]
  12. Pearson W. R., Lipman D. J. 1988; Improved tools for biological sequence comparison. Proc Natl Acad Sci U S A 85:2444–2448 [CrossRef]
    [Google Scholar]
  13. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  14. Sato Y., Nishihara H., Yoshida M., Watanabe M., Rondal J. D., Ohta H. 2004; Occurrence of hydrogen-oxidizing Ralstonia species as primary microorganisms in the Mt. Pinatubo volcanic mudflow deposits. Soil Sci Plant Nutr 50:855–861 [CrossRef]
    [Google Scholar]
  15. Sato Y., Nishihara H., Yoshida M., Watanabe M., Rondal J. D., Concepcion R. N., Ohta H. 2006 Cupriavidus pinatubonensis sp. nov. and Cupriavidus laharis sp. nov., novel hydrogen-oxidizing, facultatively chemolithotrophic bacteria isolated from volcanic mudflow deposits from Mt. Pinatubo in the Philippines. Int J Syst Evol Microbiol 56973–978 [CrossRef]
  16. Spring S., Kämpfer P., Schleifer K. H. 2001; Limnobacter thiooxidans gen. nov., sp. nov., a novel thiosulfate-oxidizing bacterium isolated from freshwater lake sediment. Int J Syst Evol Microbiol 51:1463–1470
    [Google Scholar]
  17. Tamaoka J., Komagata K. 1984; Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128 [CrossRef]
    [Google Scholar]
  18. Thompson J. D., Higgins D. G., Gibson T. J. 1994; clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680 [CrossRef]
    [Google Scholar]
  19. Ushiba Y., Takahara Y., Ohta H. 2003; Sphingobium amiense sp. nov., a novel nonylphenol-degrading bacterium isolated from a river sediment. Int J Syst Evol Microbiol 53:2045–2048 [CrossRef]
    [Google Scholar]
  20. van der Linde K., Lim B. T., Rondeel J. M. M., Antonissen L. P. M. T., de Jong G. M. T. 1999; Improved bacteriological surveillance of haemodialysis fluids: a comparison between tryptic soy agar and Reasoner's 2A media. Nephrol Dial Transplant 14:2433–2437 [CrossRef]
    [Google Scholar]
  21. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. other authors 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [CrossRef]
    [Google Scholar]
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