1887

Abstract

A Gram-negative, aerobic, heterotrophic bacterium, designated KP1-19, was isolated from a 22-year-old volcanic deposit at a site lacking vegetation on the island of Miyake, Japan. Strain KP1-19 was able to use thiosulfate (optimum concentration 10 mM) as an additional energy source. 16S rRNA gene sequence analysis indicated that strain KP1-19 was closely related to CS-K2 within the class (97.7 % 16S rRNA gene sequence similarity). The cellular fatty acid profile was characteristic of the genus : the major fatty acids (>5 %) were C, C 7 and C 7 and minor amounts of C 3-OH were also found. DNA–DNA relatedness between strain KP1-19 and LMG 19593 was 18 %. Therefore, strain KP1-19 represents a novel species, for which the name sp. nov. is proposed. The type strain is KP1-19 (=LMG 24869 =NBRC 105857 =CIP 109929).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.020206-0
2011-02-01
2019-10-23
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/61/2/404.html?itemId=/content/journal/ijsem/10.1099/ijs.0.020206-0&mimeType=html&fmt=ahah

References

  1. Ezaki, T., Hashimoto, Y. & Yabuuchi, E. ( 1989; ). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39, 224–229.[CrossRef]
    [Google Scholar]
  2. Ikemoto, S., Kathoh, K. & Komagata, K. ( 1978; ). Cellular fatty acid composition in methanol-utilizing bacteria. J Gen Appl Microbiol 24, 41–49.[CrossRef]
    [Google Scholar]
  3. King, G. M. ( 2007; ). Chemolithotrophic bacteria: distributions, functions and significance in volcanic environment. Microbes Environ 22, 309–319.[CrossRef]
    [Google Scholar]
  4. King, G. M., Weber, C. F., Nanba, K., Sato, Y. & Ohta, H. ( 2008; ). Atmospheric CO and hydrogen uptake and CO oxidizer phylogeny for Miyake-jima, Japan volcanic deposits. Microbes Environ 23, 299–305.[CrossRef]
    [Google Scholar]
  5. Komagata, K. & Suzuki, K. ( 1987; ). Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19, 161–207.
    [Google Scholar]
  6. Lu, H., Fujimura, R., Sato, Y., Nanba, K., Kamijo, T. & Ohta, H. ( 2008; ). Characterization of Herbaspirillum- and Limnobacter-related strains isolated from young volcanic deposits in Miyake-jima Island, Japan. Microbes Environ 23, 66–72.[CrossRef]
    [Google Scholar]
  7. Ohta, H. & Hattori, T. ( 1983; ). Agromonas oligotrophica gen. nov., sp. nov., a nitrogen-fixing oligotrophic bacterium. Antonie van Leeuwenhoek 49, 429–446.
    [Google Scholar]
  8. Ohta, H., Ogiwara, K., Murakami, E., Takahashi, H., Sekiguchi, M., Koshida, K., Someya, T., Morishima, W., Rondal, J. D. & other authors ( 2003; ). Quinone profiling of bacterial populations developed in the surface layer of volcanic mudflow deposits from Mt. Pinatubo (the Philippines). Soil Biol Biochem 35, 1155–1158.[CrossRef]
    [Google Scholar]
  9. Ohta, H., Hattori, R., Ushiba, Y., Mitsui, H., Ito, M., Watanabe, H., Tonosaki, A. & Hattori, T. ( 2004; ). Sphingomonas oligophenolica sp. nov., a halo- and organo-sensitive oligotrophic bacterium from paddy soil that degrades phenolic acids at low concentrations. Int J Syst Evol Microbiol 54, 2185–2190.[CrossRef]
    [Google Scholar]
  10. Ostle, A. G. & Holt, J. G. ( 1982; ). Nile blue A as a fluorescent stain for poly-beta-hydroxybutyrate. Appl Environ Microbiol 44, 238–241.
    [Google Scholar]
  11. Page, R. D. M. ( 1996; ). TreeView: an application to display phylogenetic trees on personal computers. Comput Appl Biosci 12, 357–358.
    [Google Scholar]
  12. Pearson, W. R. & Lipman, D. J. ( 1988; ). Improved tools for biological sequence comparison. Proc Natl Acad Sci U S A 85, 2444–2448.[CrossRef]
    [Google Scholar]
  13. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  14. Sato, Y., Nishihara, H., Yoshida, M., Watanabe, M., Rondal, J. D. & Ohta, H. ( 2004; ). Occurrence of hydrogen-oxidizing Ralstonia species as primary microorganisms in the Mt. Pinatubo volcanic mudflow deposits. Soil Sci Plant Nutr 50, 855–861.[CrossRef]
    [Google Scholar]
  15. Sato, Y., Nishihara, H., Yoshida, M., Watanabe, M., Rondal, J. D., Concepcion, R. N. & Ohta, H. ( 2006; ). Cupriavidus pinatubonensis sp. nov. and Cupriavidus laharis sp. nov., novel hydrogen-oxidizing, facultatively chemolithotrophic bacteria isolated from volcanic mudflow deposits from Mt. Pinatubo in the Philippines. Int J Syst Evol Microbiol 56, 973–978.[CrossRef]
    [Google Scholar]
  16. Spring, S., Kämpfer, P. & Schleifer, K. H. ( 2001; ). Limnobacter thiooxidans gen. nov., sp. nov., a novel thiosulfate-oxidizing bacterium isolated from freshwater lake sediment. Int J Syst Evol Microbiol 51, 1463–1470.
    [Google Scholar]
  17. Tamaoka, J. & Komagata, K. ( 1984; ). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25, 125–128.[CrossRef]
    [Google Scholar]
  18. Thompson, J. D., Higgins, D. G. & Gibson, T. J. ( 1994; ). clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22, 4673–4680.[CrossRef]
    [Google Scholar]
  19. Ushiba, Y., Takahara, Y. & Ohta, H. ( 2003; ). Sphingobium amiense sp. nov., a novel nonylphenol-degrading bacterium isolated from a river sediment. Int J Syst Evol Microbiol 53, 2045–2048.[CrossRef]
    [Google Scholar]
  20. van der Linde, K., Lim, B. T., Rondeel, J. M. M., Antonissen, L. P. M. T. & de Jong, G. M. T. ( 1999; ). Improved bacteriological surveillance of haemodialysis fluids: a comparison between tryptic soy agar and Reasoner's 2A media. Nephrol Dial Transplant 14, 2433–2437.[CrossRef]
    [Google Scholar]
  21. Wayne, L. G., Brenner, D. J., Colwell, R. R., Grimont, P. A. D., Kandler, O., Krichevsky, M. I., Moore, L. H., Moore, W. E. C., Murray, R. G. E. & other authors ( 1987; ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.020206-0
Loading
/content/journal/ijsem/10.1099/ijs.0.020206-0
Loading

Data & Media loading...

Supplements

vol. , part 2, pp. 404 - 407

Electron micrograph of cells of sp. nov. KP1-19 negatively stained with 1 % sodium phosphotungstate (pH 7.2). Bar, 200 nm.



IMAGE

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error