1887

Abstract

A Gram-reaction-positive, aerobic, catalase-positive, non-spore-forming, rod-shaped bacterium designated IMMIB L-1656 was isolated from an ear swab of a man and characterized using a polyphasic approach. 16S rRNA gene sequence analysis indicated that strain IMMIB L-1656 is related to members of the family (<95.1 % sequence similarity). Anaylsis using different phylogenetic algorithms consistently grouped strain IMMIB L-1656 with members of the genus . The organism posessed a cell-wall murein based on -lysine (variation A4, type -Lys–Gly–-Glu), MK-10 as the predominant menaquinone and long-chain cellular fatty acids of straight-chain and branched-chain saturated types (with iso-C and anteiso-C predominating). The polar lipids included diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol in addition to unknown glycolipids. The DNA G+C content was 59.7 mol%. Based on its distinctive genotypic and phenotypic characteristics, strain IMMIB L-1656 represents a novel species in a novel genus, for which the name gen. nov., sp. nov. is proposed. We also propose that members of the family be transferred to the family with amendments to the description of the suborder The type strain of is IMMIB L-1656 (=DSM 45359 =CCUG 57943).

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2011-02-01
2019-10-19
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References

  1. Altenburger, P., Kämpfer, P., Schumann, P., Steiner, R., Lubitz, W. & Busse, H.-J. ( 2002; ). Citricoccus muralis gen. nov., sp. nov., a novel actinobacterium isolated from a medieval wall painting. Int J Syst Evol Microbiol 52, 2095–2100.[CrossRef]
    [Google Scholar]
  2. Barrow, G. I. & Feltham, R. K. A. ( 1993; ). Cowan and Steel's Manual for the Identification of Medical Bacteria, 3rd edn. Cambridge. : Cambridge University Press.
    [Google Scholar]
  3. Bauer, A. W., Kirby, W. M. M., Sherris, J. C. & Truck, M. ( 1966; ). Antibiotic sensitivity testing by a standardized single disk method. Am J Clin Pathol 45, 493–496.
    [Google Scholar]
  4. Collins, M. D., Pirouz, T., Goodfellow, M. & Minnikin, D. E. ( 1977; ). Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 100, 221–230.[CrossRef]
    [Google Scholar]
  5. Daneshvar, M. I., Hollis, D. G., Weyant, R. S., Jordan, J. G., MacGregor, J. P., Morey, R. E., Whitney, A. M., Brenner, D. J. & Steigerwalt, A. G. & other authors ( 2004; ). Identification of some charcoal-black-pigmented CDC fermentative coryneform group 4 isolates as Rothia dentocariosa and some as Corynebacterium aurimucosum: proposal of Rothia dentocariosa emend. Georg and Brown 1967, Corynebacterium aurimucosum emend. Yassin et al. 2002, and Corynebacterium nigricans Shukla et al. 2003 pro synon. Corynebacterium aurimucosum. J Clin Microbiol 42, 4189–4198.[CrossRef]
    [Google Scholar]
  6. Facklam, R. & Elliot, J. A. ( 1995; ). Identification, classification, and clinical relevance of catalase-negative, Gram-positive cocci, excluding the streptococci and enterococci. Clin Microbiol Rev 8, 479–495.
    [Google Scholar]
  7. Felsenstein, J. ( 1981; ). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17, 368–376.[CrossRef]
    [Google Scholar]
  8. Felsenstein, J. ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef]
    [Google Scholar]
  9. Fitch, W. M. ( 1971; ). Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20, 406–416.[CrossRef]
    [Google Scholar]
  10. Funke, G., Hutson, R. A., Bernard, K. A., Pfyffer, G. E., Wauters, G. & Collins, M. D. ( 1996; ). Isolation of Arthrobacter spp. from clinical specimens and description of Arthrobacter cumminsii sp. nov. and Arthrobacter woluwensis sp. nov. J Clin Microbiol 34, 2356–2363.
    [Google Scholar]
  11. Georg, L. K. & Brown, J. M. ( 1967; ). Rothia, gen. nov. An aerobic genus of the family Actinomycetaceae. Int J Syst Bacteriol 17, 79–88.[CrossRef]
    [Google Scholar]
  12. Gerencser, M. A. & Bowden, G. H. ( 1986; ). Genus Rothia Georg and Brown 1967, 68AL. In Bergey's Manual of Systematic Bacteriology, vol. 2, pp. 1342–1346. Edited by Sneath, P. H. A., Mair, N. S., Sharpe, M. E. & Holt, J. G.. Baltimore. : Williams & Wilkins..
    [Google Scholar]
  13. Gordon, R. E. ( 1966; ). Some criteria for the recognition of Nocardia madurae (Vincent) Blanchard. J Gen Microbiol 45, 355–364.[CrossRef]
    [Google Scholar]
  14. Gordon, R. E. ( 1967; ). The taxonomy of soil bacteria. In The Ecology of Soil Bacteria, pp. 293–321. Edited by Gray, T. R. G. & Parkinson, B.. Liverpool. : Liverpool University Press.
    [Google Scholar]
  15. Gordon, R. E. & Mihm, J. M. ( 1957; ). A comparative study of some strains received as nocardiae. J Bacteriol 73, 15–27.
    [Google Scholar]
  16. Groth, I., Schumann, P., Weiss, N., Martin, K. & Rainey, F. A. ( 1996; ). Agrococcus jenensis gen. nov., sp. nov., a new genus of actinomycetes with diaminobutyric acid in the cell wall. Int J Syst Bacteriol 46, 234–239.[CrossRef]
    [Google Scholar]
  17. Jukes, T. H. & Cantor, C. R. ( 1969; ). Evolution of protein molecules. In Mammalian Protein Metabolism, vol. 3, pp. 21–132. Edited by Munro, H. N.. New York. : Academic Press.
    [Google Scholar]
  18. Lechevalier, M. P. ( 1968; ). Identification of aerobic actinomycetes of clinical importance. J Lab Clin Med 71, 934–944.
    [Google Scholar]
  19. Lechevalier, M. P., De Bièvre, C. & Lechevalier, H. A. ( 1977; ). Chemotaxonomy of aerobic actinomycetes: phospholipid composition. Biochem Syst Ecol 5, 249–260.[CrossRef]
    [Google Scholar]
  20. Li, W.-J., Chen, H.-H., Xu, P., Zhang, Y.-Q., Schumann, P., Tang, S.-K., Xu, L.-H. & Jiang, C.-L. ( 2004; ). Yania halotolerans gen. nov., sp. nov., a novel member of the suborder Micrococcineae from saline soil in China. Int J Syst Evol Microbiol 54, 525–531.[CrossRef]
    [Google Scholar]
  21. Li, W.-J., Chen, H.-H., Zhang, Y.-Q., Kim, C.-J., Park, D.-J., Lee, J.-C., Xu, L.-H. & Jiang, C.-L. ( 2005; ). Citricoccus alkalitolerans sp. nov., a novel actinobacterium isolated from a desert soil in Egypt. Int J Syst Evol Microbiol 55, 87–90.[CrossRef]
    [Google Scholar]
  22. Li, W.-J., Zhi, X.-Y. & Euzéby, J. P. ( 2008; ). Proposal of Yaniellaceae fam. nov., Yaniella gen. nov. and Sinobaca gen. nov. as replacements for the illegitimate prokaryotic names Yaniaceae Li et al. 2005, Yania Li et al. 2004, emend Li et al. 2005, and Sinococcus Li et al. 2006, respectively. Int J Syst Evol Microbiol 58, 525–527.[CrossRef]
    [Google Scholar]
  23. Ludwig, W., Strunk, O., Westram, R., Richter, L., Meier, H., Yadhukumar, Buchner, A., Lai, T., Steppi, S. & other authors ( 2004; ). arb: a software environment for sequence data. Nucleic Acids Res 32, 1363–1371.[CrossRef]
    [Google Scholar]
  24. MacKenzie, S. L. ( 1987; ). Gas chromatographic analysis of amino acids as the N-heptafluorobutyryl isobutyl esters. J Assoc Off Anal Chem 70, 151–160.
    [Google Scholar]
  25. Mages, I. S., Frodl, R., Bernard, K. A. & Funke, G. ( 2008; ). Identities of Arthrobacter spp. and Arthrobacter-like bacteria encountered in human clinical specimens. J Clin Microbiol 46, 2980–2986.[CrossRef]
    [Google Scholar]
  26. Mesbah, M., Premachandran, U. & Whitman, W. B. ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef]
    [Google Scholar]
  27. Minnikin, D. E., Hutchinson, I. G., Caldicott, A. B. & Goodfellow, M. ( 1980; ). Thin-layer chromatography of methanolysates of mycolic acid-containing bacteria. J Chromatogr A 188, 221–223.[CrossRef]
    [Google Scholar]
  28. Rainey, F. A., Ward-Rainey, N., Kroppenstedt, R. M. & Stackebrandt, E. ( 1996; ). The genus Nocardiopsis represents a phylogenetically coherent taxon and a distinct actinomycete lineage: proposal of Nocardiopsiaceae fam. nov. Int J Syst Bacteriol 46, 28–96.
    [Google Scholar]
  29. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  30. Schleifer, K. H. ( 1985; ). Analysis of the chemical composition and primary structure of murein. Methods Microbiol 18, 123–156.
    [Google Scholar]
  31. Schleifer, K. H. & Kandler, O. ( 1972; ). Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 36, 407–477.
    [Google Scholar]
  32. Schumann, P., Kämpfer, P., Busse, H.-J. & Evtushenko, L. I., Subcommittee on the Taxonomy of the Suborder Micrococcineae of the International Committee on Systematics of Prokaryotes ( 2009; ). Proposed minimal standards for describing new genera and species of the suborder Micrococcineae. Int J Syst Evol Microbiol 59, 1823–1849.[CrossRef]
    [Google Scholar]
  33. Stackebrandt, E. & Schumann, P. ( 2000; ). Description of Bogoriellaceae fam. nov., Dermacoccaceae fam. nov., Rarobacteraceae fam. nov. and Sanguibacteraceae fam. nov. and emendation of some families of the suborder Micrococcineae. Int J Syst Evol Microbiol 50, 1279–1285.[CrossRef]
    [Google Scholar]
  34. Stackebrandt, E., Lewis, B. J. & Woese, C. R. ( 1980; ). The phylogenetic structure of the coryneform group of bacteria. Zentralbl Bakteriol Parasitenkd Infektionskr Hyg Abt. 1 Orig. Reihe C 1, 137–149.
    [Google Scholar]
  35. Stackebrandt, E., Koch, C., Gvozdiak, O. & Schumann, P. ( 1995; ). Taxonomic dissection of the genus Micrococcus: Kocuria gen. nov., Nesterenkonia gen. nov., Kytococcus gen. nov., Dermacoccus gen. nov., and Micrococcus Cohn 1872 gen. emend. Int J Syst Bacteriol 45, 682–692.[CrossRef]
    [Google Scholar]
  36. Stackebrandt, E., Rainey, F. A. & Ward-Rainey, N. L. ( 1997; ). Proposal for a new hierarchic classification system, Actinobacteria classis nov. Int J Syst Bacteriol 47, 479–491.[CrossRef]
    [Google Scholar]
  37. Wieser, M., Denner, E. B. M., Kämpfer, P., Schumann, P., Tindall, B., Steiner, U., Vybiral, D., Lubitz, W., Maszenan, A. M. & other authors ( 2002; ). Emended descriptions of the genus Micrococcus, Micrococcus luteus (Cohn 1872) and Micrococcus lylae (Kloos et al. 1974). Int J Syst Evol Microbiol 52, 629–637.
    [Google Scholar]
  38. Yassin, A. F. & Hupfer, H. ( 2006; ). Williamsia deligens sp. nov., isolated from human blood. Int J Syst Evol Microbiol 56, 193–197.[CrossRef]
    [Google Scholar]
  39. Yassin, A. F., Haggenei, B., Budzikiewicz, H. & Schaal, K. P. ( 1993; ). Fatty acid and polar lipid composition of the genus Amycolatopsis: application of fast atom bombardment–mass spectrometry to structure analysis of underivatized phospholipids. Int J Syst Bacteriol 43, 414–420.[CrossRef]
    [Google Scholar]
  40. Yassin, A. F., Rainey, F. A., Brzezinka, H., Burghardt, J., Lee, H. L. & Schaal, K. P. ( 1995; ). Tsukamurella inchonensis sp. nov. Int J Syst Bacteriol 45, 522–527.[CrossRef]
    [Google Scholar]
  41. Yassin, A. F., Chen, W. M., Hupfer, H., Siering, C., Kroppenstedt, R. M., Arun, A. B., Lai, W. A., Shen, F. T., Rekha, P. D. & Young, C. C. ( 2007; ). Lysobacter defluvii sp. nov., isolated from municipal solid waste. Int J Syst Evol Microbiol 57, 1131–1136.[CrossRef]
    [Google Scholar]
  42. Zhang, Y.-Q., Schumann, P., Yu, L.-Y., Liu, H.-Y., Zhang, Y.-Q., Xu, L.-H., Stackebrandt, E., Jiang, C.-L. & Li, W.-J. ( 2007; ). Zhihengliuella halotolerans gen. nov., sp. nov., a novel member of the family Micrococcaceae. Int J Syst Evol Microbiol 57, 1018–1023.[CrossRef]
    [Google Scholar]
  43. Zhi, X.-Y., Li, W.-J. & Stackebrandt, E. ( 2009; ). An update of the structure and 16S rRNA gene sequence-based definition of higher ranks of the class Actinobacteria, with the proposal of two new suborders and four new families and emended descriptions of the existing higher taxa. Int J Syst Evol Microbiol 59, 589–608.[CrossRef]
    [Google Scholar]
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vol. , part 2, pp. 223 - 230

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vol. , part 2, pp. 223 - 230

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