1887

Abstract

Eight isolates originating from the marine corals and and the zoanthids and in Brazil and the Pacific white shrimp () in Ecuador were studied by means of a polyphasic approach. The novel isolates formed a tight monophyletic group in the genus and were closely related to species of the group, to which they showed more than 99 % 16S rRNA gene sequence similarity. Analysis based on concatenated sequences of the following seven genes, 16S rRNA, , , , , and (5633 bp in length), showed clear separation between the isolates and species of the group. Amplified fragment length polymorphism (AFLP) analysis, performed previously, revealed that a representative isolate of this group, LMG 20370, was clearly separate from known species (it belonged to the so-called AFLP cluster A31). DNA–DNA hybridization (DDH) experiments with representative isolates and type strains of the species group revealed high DDH between the novel isolates (more than 74 %) and less than 70 % DDH towards type strains of related species, proving the novel species status of the isolates. Phenotypically, the novel species belongs to the arginine dihydrolase (A)-negative, lysine decarboxylase (L)-positive and ornithine decarboxylase (O)-positive (A−/L+/O+) cluster reported previously. Most species of the group (i.e. , , and ) also belong to this A−/L+/O+ cluster. However, several phenotypic features can be used for the identification of the novel species. In contrast to its closest phylogenetic neighbours, the novel species exhibits esterase (C4) and -acetyl--glucosaminidase activities, but it does not produce acetoin, does not use citrate, -ketoglutaric acid or propionic acid and does not ferment melibiose. The novel species can also be differentiated on the basis of the presence of the fatty acids C C 8, iso-C and iso-C and the absence of the fatty acid C. The name sp. nov. is proposed for this taxon. Strain R-40496 (=LMG 25430 =CAIM 1816) is the type strain.

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2011-02-01
2020-01-17
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Supplements

vol. , part 2, pp. 362 - 368

GenBank accession numbers of the housekeeping gene sequences used in this study.

Sequence similarity between the 16S rRNA, , , , , and gene sequences of sp. nov. R-40496 and type strains of related species.

DNA–DNA hybridization and DNA G+C contents of strains examined in this study.

Phenotypic variability among strains of sp. nov.

Cellular fatty acid contents of strains of sp. nov. and their closest phylogenetic neighbours.

Phylogenetic tree based on the maximum-parsimony method, using partial 16S rRNA gene sequences (1470 bp).

Neighbour-joining phylogenetic trees showing the position of strains of sp. nov. based on sequences of (775 bp) (S2), (556 bp) (S3), (790 bp) (S4), (654 bp) (S5), (531 bp) (S6) and (857 bp) (S7).

Neighbour-joining phylogenetic tree showing the phylogenetic position of strains of sp. nov. based on concatenated 16S rRNA (1470 bp), (775 bp), (556 bp), (790 bp), (654 bp), (531 bp) and (857 bp) gene sequences.

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