1887

Abstract

Eight isolates originating from the marine corals and and the zoanthids and in Brazil and the Pacific white shrimp () in Ecuador were studied by means of a polyphasic approach. The novel isolates formed a tight monophyletic group in the genus and were closely related to species of the group, to which they showed more than 99 % 16S rRNA gene sequence similarity. Analysis based on concatenated sequences of the following seven genes, 16S rRNA, , , , , and (5633 bp in length), showed clear separation between the isolates and species of the group. Amplified fragment length polymorphism (AFLP) analysis, performed previously, revealed that a representative isolate of this group, LMG 20370, was clearly separate from known species (it belonged to the so-called AFLP cluster A31). DNA–DNA hybridization (DDH) experiments with representative isolates and type strains of the species group revealed high DDH between the novel isolates (more than 74 %) and less than 70 % DDH towards type strains of related species, proving the novel species status of the isolates. Phenotypically, the novel species belongs to the arginine dihydrolase (A)-negative, lysine decarboxylase (L)-positive and ornithine decarboxylase (O)-positive (A−/L+/O+) cluster reported previously. Most species of the group (i.e. , , and ) also belong to this A−/L+/O+ cluster. However, several phenotypic features can be used for the identification of the novel species. In contrast to its closest phylogenetic neighbours, the novel species exhibits esterase (C4) and -acetyl--glucosaminidase activities, but it does not produce acetoin, does not use citrate, -ketoglutaric acid or propionic acid and does not ferment melibiose. The novel species can also be differentiated on the basis of the presence of the fatty acids C C 8, iso-C and iso-C and the absence of the fatty acid C. The name sp. nov. is proposed for this taxon. Strain R-40496 (=LMG 25430 =CAIM 1816) is the type strain.

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2011-02-01
2020-10-01
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