1887

Abstract

A bacterial strain, designated BF-3, was isolated from phenol-contaminated soil and investigated using a polyphasic taxonomic approach. Cells were Gram-reaction-negative, non-sporulating, non-motile, short rods. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain BF-3 formed a monophyletic branch at the periphery of the evolutionary radiation occupied by the genus ; it showed highest sequence similarities to LMG 2370 (96.8 %), DSM 13191 (96.4 %), LMG 23579 (96.4 %), LMG 3475 (96.3 %), KCTC 12005 (96.1 %) and LMG 1253 (96.0 %). The major cellular fatty acids were C, C/C 7, C cyclo and summed feature 3 (C 7 and/or iso-C 2-OH). Based on the phylogenetic analysis, DNA–DNA hybridization, whole-cell fatty acid composition and biochemical characteristics, strain BF-3 was clearly distinct from type strains of other recognized species of the genus and, as such, represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is BF-3 (=CCTCC AB 209170 =DSM 22523).

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2011-02-01
2019-10-14
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Phylogenetic trees based on complete 16S rRNA gene sequences of strain BF-3 and related species of the genus , reconstructed using the Maximum-parsimony (Fig. S1) and Maximum-likelihood (Fig. S2) methods. [PDF](30KB)

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Genotypic, phenotypic and nutritional characteristics (Table S1) and cellular fatty acid compositions (Table S2) that distinguish strain BF-3 from related species of the genus [PDF](34KB)

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