1887

Abstract

A Gram-negative bacterium, designated B2-9, was isolated from water of a slightly alkaline lake, Longhu Lake in Daqing, north-east China. Strain B2-9 was an aerobic rod, heterotrophic, catalase- and oxidase-positive and motile by means of a single polar flflagellum. The isolate grew well on n-alkanes C–C and weakly on C–C, C and C. Growth occurred at 10–37 °C (optimum 20–25 °C), at pH 5.5–9.5 (optimum pH 7.0) and with ≤1.0 % (w/v) NaCl. The major fatty acids were C 7 (41.4 %), summed feature 3 (iso-C 2-OH and/or C 7; 18.5 %) and C (10.9 %). Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain B2-9 belonged to the genus of the class and shared 97.0 % 16S rRNA gene sequence similarity with AP103. DNA–DNA relatedness between strain B2-9 and . DSM 16095 was 24 %. The DNA G+C content of strain B2-9 was 66 mol%. Strain B2-9 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is B2-9 (=CGMCC 1.7049 =NBRC 104238).

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2011-02-01
2019-12-08
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vol. , part 2, pp. 408 - 411

Transmission electron micrographs of sp. nov. B2-9 , grown on R2A for 1 day (a) and 3 days (b and c), and DSM 16095 , grown on R2A for 3 days (d). (a) Group of single cells; (b) single polar flagellum and vesicles in the culture fluid; (c) cell covered with vesicles which are formed by local extensions of the outer membrane; (d) single polar flagellum without vesicles. Bars, 1.0 µm (a, d), 0.5 µm (b), 0.2 µm (c).

Cellular fatty acid compositions of strain B2-9 and DSM 16095 Fatty acids that represented <1% of the total in both strains are not shown. All data were taken from this study. tr, Trace (<1.0%); nd, not detected.

*Summed features represent two or three fatty acids that cannot be separated by the Microbial Identification System. Summed feature 2 consisted of C 3-OH and/or iso-C I. Summed feature 3 consisted of iso-C 2-OH and/or C ω7 .



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