1887

Abstract

A Gram-negative, oxidase- and catalase-positive, flagellated, rod-shaped bacterium, designated strain EM 1, was isolated from purified water. 16S rRNA gene sequence analysis indicated that the novel strain belonged to the family within the class ; the closest phylogenetic relative was DSM 19792 (96.7 % gene sequence similarity). The new isolate could be distinguished from the type strain of DSM 19792 (=CCUG 49009=CIP 109318) and from strain CCUG 49012, which has been described as a second genomovar of this species, on the basis of genotypic data and several phenotypic properties. An S-layer was present in the cell envelope in DSM 19792, but was absent in strains EM 1 and CCUG 49012. Test conditions were established that enabled strain CCUG 49012 to be distinguished from DSM 19792. As found for , the main fatty acids of strains EM 1 and CCUG 49012 were summed feature 3 (including unsaturated C 7), straight-chain C and unsaturated C 7 (low percentage in strain CCUG 49012), and C 3-OH was the sole hydroxylated fatty acid. The polar lipid profile consisted of the predominant lipids phosphatidylethanolamine, diphosphatidylglycerol and phosphatidylglycerol. The polyamine profile was mainly composed of the major compound putrescine and moderate amounts of 2-hydroxyputrescine. In contrast to and strain CCUG 49012, where ubiquinone Q8 was reported as the sole quinone component, the quinone system of strain EM 1 consisted of ubiquinone Q-8 (64 %) and Q-7 (36 %). The 16S rRNA gene sequence similarity, the polar lipid profile and the absence of C-hydroxylated fatty acids all indicated that strain EM 1 was affiliated with the genus . 16S rRNA gene sequence similarity values lower than 97.0 % and several differentiating phenotypic traits demonstrated that strain EM 1 represents a novel species for which the name sp. nov. is proposed (type strain EM 1=DSM 21777=CCUG 57265). In addition, based on previously published results and this study, a separate species, sp. nov., is proposed with strain CCUG 49012 (=DSM 23061=CIP 109317) as the type strain.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.018648-0
2011-02-01
2019-10-18
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/61/2/384.html?itemId=/content/journal/ijsem/10.1099/ijs.0.018648-0&mimeType=html&fmt=ahah

References

  1. Altenburger, P., Kämpfer, P., Makristathis, A., Lubitz, W. & Busse, H.-J. ( 1996; ). Classification of bacteria isolated from a medieval wall painting. J Biotechnol 47, 39–52.[CrossRef]
    [Google Scholar]
  2. Busse, H.-J. & Auling, G. ( 1988; ). Polyamine pattern as a chemotaxonomic marker within the Proteobacteria. Syst Appl Microbiol 11, 1–8.[CrossRef]
    [Google Scholar]
  3. Cowan, S. T. & Steel, K. J. ( 1974; ). Manual for the identification of medical bacteria, 2nd edn. Cambridge. : Cambridge University Press.
    [Google Scholar]
  4. Fernandes, C., Rainey, F. A., Nobre, M. F., Pinhal, I., Folhas, F. & da Costa, M. S. ( 2005; ). Herminiimonas fonticola gen. nov. sp. nov., a betaproteobacterium isolated from a source of bottled mineral water. Syst Appl Microbiol 28, 596–603.[CrossRef]
    [Google Scholar]
  5. Fluharty, D. M. & Packard, M. ( 1967; ). Differentiation of Gram-positive and Gram-negative bacteria without staining. Am J Vet Clin Pathol 1, 31–35.
    [Google Scholar]
  6. Garrity, G. M., Bell, J. A. & Lilburn, T. ( 2005; ). Family II. Oxalobacteraceae fam. nov. In Bergey's Manual of Systematic Bacteriology, 2nd edn, vol. 2C, p. 623. Edited by Brenner, D. J., Krieg, N. R., Staley, J. T. & Garrity, G. M.. Heidelberg & New York. : Springer.
    [Google Scholar]
  7. Huisman, G. W., Siegele, D., Zambrano, M. M. & Kolter, R. ( 1996; ). Morphological and physiological changes during stationary phase. In Escherichia coli and Salmonella. Cellular and Molecular Biology, 2nd edn, vol. 1, p. 1672. Edited by Neidhardt, F. C.. Washington, D.C.. : American Society for Microbiology.
    [Google Scholar]
  8. Kämpfer, P. & Kroppenstedt, R. M. ( 1996; ). Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. Can J Microbiol 42, 989–1005.[CrossRef]
    [Google Scholar]
  9. Kämpfer, P., Steiof, M. & Dott, W. ( 1991; ). Microbiological characterization of a fuel-oil contaminated site including numerical identification of heterotrophic water and soil bacteria. Microb Ecol 21, 227–251.[CrossRef]
    [Google Scholar]
  10. Kämpfer, P., Rosselló-Mora, R., Hermansson, M., Persson, F., Huber, B., Falsen, E. & Busse, H.-J. ( 2007; ). Undibacterium pigrum gen. nov., sp. nov., isolated from drinking water. Int J Syst Evol Microbiol 57, 1510–1515.[CrossRef]
    [Google Scholar]
  11. Ludwig, W., Strunk, O., Westram, R., Richter, L., Meier, H., Yadhukumar, Buchner, A., Lai, T., Steppi, S. & other authors (( 2004; ). ). arb: a software environment for sequence data. Nucleic Acids Res 32, 1363–1371.[CrossRef]
    [Google Scholar]
  12. Mesbah, M., Premachandran, U. & Whitman, W. B. ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef]
    [Google Scholar]
  13. Pruesse, E., Quast, C., Knittel, K., Fuchs, B., Ludwig, W., Peplies, J. & Glöckner, F. O. ( 2007; ). silva: a comprehensive online resource for quality checked and aligned ribosomal RNA sequence data compatible with arb. Nucleic Acids Res 35, 7188–7196.[CrossRef]
    [Google Scholar]
  14. Sasser, M. ( 1990; ). Identification of bacteria by gas chromatography of cellular fatty acids. USFCC Newsl 20, 16.
    [Google Scholar]
  15. Stamatakis, A. ( 2006; ). RAxML-VI-HPC: Maximum likelihood-based phylogenetic analyses with thousands of taxa and mixed models. Bioinformatics 22, 2688–2690.[CrossRef]
    [Google Scholar]
  16. Stolz, A., Busse, H.-J. & Kämpfer, P. ( 2007; ). Pseudomonas knackmussii sp. nov. Int J Syst Evol Microbiol 57, 572–576.[CrossRef]
    [Google Scholar]
  17. Tamaoka, J. & Komagata, K. ( 1984; ). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25, 125–128.[CrossRef]
    [Google Scholar]
  18. Tindall, B. J. ( 1990a; ). A comparative study of the lipid composition of Halobacterium saccharovorum from various sources. Syst Appl Microbiol 13, 128–130.[CrossRef]
    [Google Scholar]
  19. Tindall, B. J. ( 1990b; ). Lipid composition of Halobacterium lacusprofundi. FEMS Microbiol Lett 66, 199–202.[CrossRef]
    [Google Scholar]
  20. Xu, P., Li, W.-J., Tang, S.-K., Zhang, Y.-Q., Chen, G.-Z., Chen, H.-H., Xu, L.-H. & Jiang, C.-L. ( 2005; ). Naxibacter alkalitolerans gen. nov., sp. nov., a novel member of the family ‘Oxalobacteraceae’ isolated from China. Int J Syst Evol Microbiol 55, 1149–1153.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.018648-0
Loading
/content/journal/ijsem/10.1099/ijs.0.018648-0
Loading

Data & Media loading...

Supplements

Overall 16S rRNA gene sequence similarity values for strain EM 1 and type strains of the family . Whole-cell fatty acid composition of strain EM1 and closely related strains. [ Combined PDF file] 50 KB

PDF

Combined PDF file 

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error