1887

Abstract

A Gram-negative, motile, rod-shaped bacterial strain, designated S3-22, was isolated from a sediment sample collected from a ballast water tank of a commercial ship and subjected to a polyphasic taxonomic characterization. The isolate formed small, light-yellow, semi-translucent and circular colonies on solid complex media. The strain was oxidase- and catalase-positive and metabolized a large number of carbon sources. Chemotaxonomic analysis showed ubiquinone Q-10 as predominant respiratory quinone, phosphatidylglycerol and an unidentified glycolipid as major polar lipids and iso-C 9, iso-C, C 7 and/or iso-C 2-OH, C, iso-C and C 7 as major fatty acids and the hydroxy fatty acids iso-C 3-OH and C 3-OH. The genomic DNA G+C content was 54.9 mol%. 16S rRNA gene sequence analysis revealed that the isolate has 96.1 % similarity to the type strain of , the sole described species within the order , and less than 91.0 % similarity to other recognized species. On the basis of phenotypic and genotypic data, strain S3-22 represents a novel species of the genus , for which the name sp. nov. is proposed, with the type strain S3-22 (=CGMCC 1.9109 =JCM 16261). An emended description of the genus is also presented.

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2011-02-01
2020-01-23
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Transmission electron micrographs of cells of strain S3-22 . (a) Micrograph showing long rods with flagella. (b) Ultrathin section of exponentially grown cells. Bars, 1 µm (a) and 0.2 µm (b).

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Phylogenetic trees based on 16S rRNA gene sequences reconstructed using the maximum-parsimony method (Fig. S2) and the maximum-likelihood method (Fig. S3). [PDF](61 KB)

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Two-dimensional TLC after staining with molybdatophosphoric acid showing the total polar lipid profiles of strain S3-22 (a) and JCM 12864 (b). PG, Phosphatidylglycerol; GL1–GL5, unidentified glycolipids; L1–L13, unidentified lipids.

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Fatty acid compositions of strain S3-22 and JCM 12864 [PDF](32 KB)

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