1887

Abstract

Two freshwater isolates (WB4.1-19 and WB4.4-101), sharing 99.9 % 16S rRNA gene sequence similarity, were highly related to (99.7 % similarity; 6 bp differences). A phylogenetic tree derived from a multi-locus phylogenetic analysis (MLPA) of the concatenated sequences of five housekeeping genes (, , , and ; 3684 bp) revealed that both strains clustered as an independent phylogenetic line next to members of and . The DNA–DNA reassociation value between the two new isolates was 89.3 %. Strain WB4.1-19 had a DNA–DNA relatedness value of <70 % with the type strains of the other species tested. Phenotypic characterization differentiated the two novel strains from all other type strains of species of the genus . It is concluded that the two new strains represent a novel species of the genus , for which the name sp. nov. is proposed, with the type strain WB4.1-19 (=CECT 7518=DSM 22539=MDC 2511).

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2011-02-01
2019-10-24
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Unrooted maximum-parsimony phylogenetic tree derived from the 16S rRNA gene sequences showing relationships of strains WB4.1-19 and WB4.4-101 with all other species. Numbers at nodes indicate bootstrap values >50% (percentage of 1000 replicates). Bar, 1 base change between nodes.

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Electron microscopy images of cells of strain WB4.1-19 . (a), Transmission electron microscope, negatively stained. Bar, 500 nm. (b), Scanning electron micrograph, Bar 5 µm.

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Base differences in the 16S rRNA gene sequence between strain WB4.1-19 and WB4.4-101 and the type strains of , and . DNA–DNA relatedness (%) between strains WB4.1-19 and WB4.4-101 and , , , , , , , , and type strains. Combined [ PDF file] 31KB

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