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Abstract

Two Gram-stain-positive, mycelium-forming actinobacteria (strains 14-Be-013 and 02-Gi-014) were isolated from walls colonized with moulds and studied taxonomically. The isolates formed yellowish-pigmented substrate mycelium showing no fragmentation. Comparative analysis of 16S rRNA gene sequences showed that these bacteria are most closely related to genera within the family , but form a separate lineage within this family. Highest sequence similarities were to the type strains of (96.0 % to 14-Be-013), subsp. and (both 95.3 % to 14-Be-013). Whole-cell hydrolysates contained -diaminopimelic acid as the diagnostic diamino acid of the cell wall and no diagnostic sugars. Mycolic acids were absent. The major menaquinones were MK-10(H), MK-11(H) and MK-12(H). The polar lipid profile consisted of phosphatidylcholine, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and unknown lipids. Major fatty acids iso-C, anteiso-C and C 9 supported the affiliation of these isolates to the family . Phenotypic analysis (including chemotaxonomy) further differentiated strains 14-Be-013 and 02-Gi-014 from the most closely related members of the genera and . Since the two strains form a distinct lineage in the 16S rRNA gene sequence-based phylogenetic tree, the novel genus gen. nov. with the type species sp. nov. is proposed. The type strain of is 14-Be-013 (=DSM 45312 =CCM 7612).

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2010-08-01
2019-10-16
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vol. , part 8, pp. 1729 - 1734

Physiological and biochemical characteristics of strains 14-Be-013 and 02-Gi-014 in comparison with the type strains of the most closely related species and . [PDF](94 KB)



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