1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 60, Issue 8

gen. nov., sp. nov., an actinomycete isolated from indoor walls Free

Abstract

Two Gram-stain-positive, mycelium-forming actinobacteria (strains 14-Be-013 and 02-Gi-014) were isolated from walls colonized with moulds and studied taxonomically. The isolates formed yellowish-pigmented substrate mycelium showing no fragmentation. Comparative analysis of 16S rRNA gene sequences showed that these bacteria are most closely related to genera within the family , but form a separate lineage within this family. Highest sequence similarities were to the type strains of (96.0 % to 14-Be-013), subsp. and (both 95.3 % to 14-Be-013). Whole-cell hydrolysates contained -diaminopimelic acid as the diagnostic diamino acid of the cell wall and no diagnostic sugars. Mycolic acids were absent. The major menaquinones were MK-10(H), MK-11(H) and MK-12(H). The polar lipid profile consisted of phosphatidylcholine, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and unknown lipids. Major fatty acids iso-C, anteiso-C and C 9 supported the affiliation of these isolates to the family . Phenotypic analysis (including chemotaxonomy) further differentiated strains 14-Be-013 and 02-Gi-014 from the most closely related members of the genera and . Since the two strains form a distinct lineage in the 16S rRNA gene sequence-based phylogenetic tree, the novel genus gen. nov. with the type species sp. nov. is proposed. The type strain of is 14-Be-013 (=DSM 45312 =CCM 7612).

  • Published Online:
SGM
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.015990-0
2010-08-01
2023-12-01
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/60/8/1729.html?itemId=/content/journal/ijsem/10.1099/ijs.0.015990-0&mimeType=html&fmt=ahah

References

  1. Al-Tai A. M., Ruan J. S. 1994; Nocardiopsis halophila sp. nov., a new halophilic actinomycete isolated from soil. Int J Syst Bacteriol 44:474–478 [CrossRef]
     [Google Scholar]
  2. Al-Zarban S. S., Abbas I., Al-Musallam A. A., Steiner U., Stackebrandt E., Kroppenstedt R. M. 2002; Nocardiopsis halotolerans sp. nov., isolated from salt marsh soil in Kuwait. Int J Syst Evol Microbiol 52:525–529
     [Google Scholar]
  3. Becker B., Lechevalier M. P., Lechevalier H. A. 1965; Chemical composition of cell-wall preparations from strains of various form-genera of aerobic actinomycetes. Appl Microbiol 13:236–243
     [Google Scholar]
  4. Cai M., Zhi X.-Y., Tang S.-K., Zhang Y.-Q., Xu L.-H., Li W.-J. 2008; Streptomonospora halophila sp. nov., a halophilic actinomycete isolated from a hypersaline soil. Int J Syst Evol Microbiol 58:1556–1560 [CrossRef]
     [Google Scholar]
  5. Chakrabarty A. M., Brown J. F. 1978; Microbial genetic engineering by natural plasmid transfer. In Genetic Engineering pp 185–193 Edited by M A. Chakrabarty. West Palm; Beach, FL: CRC Press;
     [Google Scholar]
  6. Chen Y.-G., Cui X.-L., Kroppenstedt R. M., Stackebrandt E., Wen M.-L., Yu L.-H., Jiang C.-L. 2008; Nocardiopsis quinghaiensis sp. nov., isolated from saline soil in China. Int J Syst Evol Microbiol 58:699–705 [CrossRef]
     [Google Scholar]
  7. Chen Y.-G., Wang Y.-X., Zhang Y.-Q., Tang S.-K., Liu Z.-X., Xiao H.-D., Xu L.-H., Cui X.-L., Li W.-X. 2009; Nocardiopsis litoralis sp. nov., a halophilic marine actinomycete isolated from a sea anemone. Int J Syst Evol Microbiol 59:2708–2713 [CrossRef]
     [Google Scholar]
  8. Chun J., Bae K. S., Moon E. Y., Jung S.-O., Lee H. K., Kim S.-J. 2000; Nocardiopsis kunsanensis sp. nov., a moderately halophilic actinomycete isolated from a saltern. Int J Syst Evol Microbiol 50:1909–1913
     [Google Scholar]
  9. Collins M. D., Jones D. 1980; Lipids in the classification and identification of coryneform bacteria containing peptidoglycans based on 2,4-diaminobutyric acid. J Appl Bacteriol 48:459–470 [CrossRef]
     [Google Scholar]
  10. Collins M. D., Goodfellow M., Minnikin D. E. 1979; Isoprenoid quinones in the classification of coryneform and related bacteria. J Gen Microbiol 110:127–136 [CrossRef]
     [Google Scholar]
  11. Cui X. L., Mao P. H., Zeng M., Li W. J., Zhang L. P., Xu L. H., Jiang C. L. 2001; Streptimonospora salina gen. nov., sp. nov., a new member of the family Nocardiopsaceae . Int J Syst Evol Microbiol 51:357–363
     [Google Scholar]
  12. Evtushenko L. I., Taran V. V., Akimov V. N., Kroppenstedt R. M., Tiedje J. M., Stackebrandt E. 2000; Nocardiopsis tropica sp. nov., Nocardiopsis trehalosi sp. nov., nom. rev. and Nocardiopsisdassonvillei subsp. albirubida subsp. nov., comb. nov. Int J Syst Evol Microbiol 50: 73–81 [CrossRef]
     [Google Scholar]
  13. Gauze G. F., Preobrazhenskaya T. P., Sveshnikova M. A., Terekova L. P., Maksimova T. 1983; Opredelitel'Aktinomycetov. Rody Streptomyces, Streptoverticillium, Chainia . Moscow: Izd; Nauka (in Russian
  14. Groth I., Schumann P., Weiss N., Martin K., Rainey F. A. 1996; Agrococcus jenensis gen. nov., sp. nov. a new genus of actinomycetes with diaminobutyric acid in the cell wall. Int J Syst Bacteriol 46:234–239 [CrossRef]
     [Google Scholar]
  15. Grund E., Kroppenstedt R. M. 1990; Chemotaxonomy and numerical taxonomy of the genus Nocardiopsis Meyer 1976. Int J Syst Bacteriol 40:5–11 [CrossRef]
     [Google Scholar]
  16. Hozzein W. N., Li W.-J., Ali M. I. A., Hammouda O., Mousa A. S., Xu L.-H., Jiang C.-L. 2004; Nocardiopsis alkaliphila sp. nov., a novel alkaliphilic actinomycete isolated from desert soil in Egypt. Int J Syst Evol Microbiol 54:247–252 [CrossRef]
     [Google Scholar]
  17. Jones K. L. 1949; Fresh isolates of actinomycetes in which the presence of sporogenous aerial mycelia is a fluctuating characteristic. J Bacteriol 57:141–145
     [Google Scholar]
  18. Kämpfer P., Kroppenstedt R. M. 1996; Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. Can J Microbiol 42:989–1005 [CrossRef]
     [Google Scholar]
  19. Kämpfer P., Kroppenstedt R. M. 2004; Pseudonocardia benzenivorans sp. nov. Int J Syst Evol Microbiol 54:749–751 [CrossRef]
     [Google Scholar]
  20. Kämpfer P., Steiof M., Dott W. 1991; Microbiological characterization of a fuel-oil contaminated site including numerical identification of heterotrophic water and soil bacteria. Microb Ecol 21:227–251 [CrossRef]
     [Google Scholar]
  21. Kämpfer P., Busse H.-J., Rainey F. A. 2002; Nocardiopsis compostus sp. nov., from the atmosphere of a composting facility. Int J Syst Evol Microbiol 52:621–627
     [Google Scholar]
  22. Kämpfer P., Dreyer U., Neef A., Dott W., Busse H.-J. 2003; Chryseobacterium defluvii sp. nov., isolated from wastewater. Int J Syst Evol Microbiol 53:93–97 [CrossRef]
     [Google Scholar]
  23. Kroppenstedt R. M., Evtushenko L. I. 2006; The family Nocardiopsaceae . In The Prokaryotes : A Handbook on the Biology of Bacteria . , 3rd edn. vol 3 pp 754–795 Edited by Dworkin M., Falkow S., Rosenberg E., Schleifer K. H., Stackebrandt E. New York: Springer;
  24. Kukolya J., Nagy I., Laday M., Toth E., Oravecz O., Marialigeti K., Hornok L. 2002; Thermobifida cellulolytica sp. nov., a novel lignocellulose-decomposing actinomycete. Int J Syst Evol Microbiol 52:1193–1199 [CrossRef]
     [Google Scholar]
  25. Lechevalier M. P. 1968; Identification of aerobic actinomycetes of clinical importance. J Lab Clin Med 71:934–944
     [Google Scholar]
  26. Lechevalier M. P., Lechevalier H. A. 1970; Chemical composition as a criterion in the classification of aerobic actinomycetes. Int J Syst Bacteriol 20:435–443 [CrossRef]
     [Google Scholar]
  27. Li M.-G., Li W.-J., Xu P., Cui X.-L., Xu L.-H., Jiang C.-L. 2003a; Nocardiopsis xinjiangensis sp. nov., a halophilic actinomycete isolated from a saline soil sample in China. Int J Syst Evol Microbiol 53:317–321 [CrossRef]
     [Google Scholar]
  28. Li W.-J., Xu P., Zhang L.-P., Tang S.-K., Cui X.-L., Mao P.-H., Xu L.-H., Schumann P., Stackebrandt E., Jiang C.-L. 2003b; Streptomonospora alba sp. nov., a novel halophilic actinomycete, and emended description of the genus Streptomonospora Cui et al. 2001. Int J Syst Evol Microbiol 53:1421–1425 [CrossRef]
     [Google Scholar]
  29. Li W.-J., Park D.-J., Tang S.-K., Wang D., Lee J.-C., Xu L.-H., Kim C.-J., Jiang C.-L. 2004; Nocardiopsis salina sp. nov., a novel halophilic actinomycete isolated from saline soil in China. Int J Syst Evol Microbiol 54:1805–1809 [CrossRef]
     [Google Scholar]
  30. Li W.-J., Kroppenstedt R. M., Wang D., Tang S.-K., Lee J.-C., Park D.-J., Kim C.-J., Xu L.-H., Jiang C.-L. 2006; Five novel species of the genus Nocardiopsis isolated from hypersaline soils and emended description of Nocardiopsis salina Li et al. 2004. Int J Syst Evol Microbiol 56:1089–1096 [CrossRef]
     [Google Scholar]
  31. Ludwig W., Strunk O., Westram R., Richter L., Meier H., Yadhukumar Buchner A., Lai T., Steppi S. other authors 2004; arb: a software environment for sequence data. Nucleic Acids Res 32:1363–1371 [CrossRef]
     [Google Scholar]
  32. Meyer J. 1976; Nocardiopsis , a new genus of the order Actinomycetales . Int J Syst Bacteriol 26:487–493 [CrossRef]
     [Google Scholar]
  33. Minnikin D. E., Alshamaony L., Goodfellow M. 1975; Differentiation of Mycobacterium , Nocardia , and related taxa by thin-layer chromatographic analysis of whole-organism methanolysates. J Gen Microbiol 88:200–204 [CrossRef]
     [Google Scholar]
  34. Minnikin D. E., Collins M. D., Goodfellow M. 1979; Fatty acid and polar lipid composition in the classification of Cellulomonas , Oerskovia and related taxa. J Appl Bacteriol 47:87–95 [CrossRef]
     [Google Scholar]
  35. Olsen G. J., Matsuda H., Hagström R., Overbeek R. 1994; fastDNAml: a tool for construction of phylogenetic trees of DNA sequences using maximum likelihood. Comput Appl Biosci 10:41–48
     [Google Scholar]
  36. Peltola J. S. P., Andersson M. A., Kämpfer P., Auling G., Kroppenstedt R. M., Busse H.-J., Salinoja-Salonen M. S., Rainey F. A. 2001; Isolation of toxigenic Nocardiopsis strains from indoor environments and description of two new Nocardiopsis species, N. exhalans sp. nov. and N. umidischolae sp. nov. Appl Environ Microbiol 67:4293–4304 [CrossRef]
     [Google Scholar]
  37. Prauser H., Falta R. 1968; Phagensensibilität, Zellwand-Zusammensetzung und Taxonomie von Actinomyceten. Z Allg Mikrobiol 8:39–46 in German [CrossRef]
     [Google Scholar]
  38. Pruesse E., Quast C., Knittel K., Fuchs B. M., Ludwig W., Peplies J., Glöckner F. O. 2007; silva: a comprehensive online resource for quality checked and aligned ribosomal RNA sequence data compatible with arb. Nucleic Acids Res 35:7188–7196 [CrossRef]
     [Google Scholar]
  39. Sabry S. A., Ghanem N. B., Abu-Ella G. A., Schumann P., Stackebrandt E., Kroppenstedt R. M. 2004; Nocardiopsis aegyptia sp. nov., isolated from marine sediment. Int J Syst Evol Microbiol 54:453–456 [CrossRef]
     [Google Scholar]
  40. Schippers A., Bosecker K., Willscher S., Spröer C., Schumann P., Kroppenstedt R. M. 2002; Nocardiopsis metallicus sp. nov., a metal-leaching actinomycete isolated from an alkaline slag dump. Int J Syst Evol Microbiol 52:2291–2295 [CrossRef]
     [Google Scholar]
  41. Schleifer K. H., Kandler O. 1972; Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 36:407–477
     [Google Scholar]
  42. Shirling E. B., Gottlieb D. 1966; Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16:313–340 [CrossRef]
     [Google Scholar]
  43. Tamura K., Dudley J., Nei M., Kumar S. 2007; mega4: molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol 24:1596–1599 [CrossRef]
     [Google Scholar]
  44. Tang S.-K., Tian X.-P., Zhi X.-Y., Cai M., Wu J.-Y., Yang L.-L., Xu L.-H., Li W.-J. 2008; Haloactinospora alba gen. nov., sp. nov., a halophilic filamentous actinomycete of the family Nocardiopsaceae . Int J Syst Evol Microbiol 58:2075–2080 [CrossRef]
     [Google Scholar]
  45. Tian X.-P., Tang S.-K., Dong J.-D., Zhang Y.-Q., Xu L.-H., Zhang S., Li W.-J. 2009; Marinactinospora thermotolerans gen. nov., sp. nov. a marine actinomycete isolated from a sediment in the northern South China sea. Int J Syst Evol Microbiol 59:948–952 [CrossRef]
     [Google Scholar]
  46. Yang R., Zhang L.-P., Guo L.-G., Shi N., Lu Z., Zhang X. 2008a; Nocardiopsis valliformis sp. nov., an alkaliphilic actinomycete isolated from alkali lake soil in China. Int J Syst Evol Microbiol 58:1542–1546 [CrossRef]
     [Google Scholar]
  47. Yang L.-L., Tang S.-K., Zhang Y.-Q., Zhi X.-Y., Wang D., Xu L.-H., Li W.-J. 2008b; Thermobifida halotolerans sp. nov., isolated from a salt mine sample, and emended description of the genus Thermobifida . Int J Syst Evol Microbiol 58:1821–1825 [CrossRef]
     [Google Scholar]
  48. Yassin A. F., Galinski E. A., Wohlfarth A., Jahnke K.-D., Schaal K.-P., Trüper H. G. 1993; A new actinomycete species, Nocardiopsis lucentensis sp. nov. Int J Syst Bacteriol 43:266–271 [CrossRef]
     [Google Scholar]
  49. Yassin A. F., Rainey F. A., Burghardt J., Gierth D., Ungerechts J., Lux I., Seifert P., Bal C., Schaal K.-P. 1997; Description of Nocardiopsis synnemataformans sp. nov., elevation of Nocardiopsis alba subsp. prasina to Nocardiopsis prasina comb. nov., and designation of Nocardiopsis antarctica and Nocardiopsis alborubida as later subjective synonyms of Nocardiopsis dassonvillei . Int J Syst Bacteriol 47:983–988 [CrossRef]
     [Google Scholar]
  50. Yassin A. F., Spröer C., Hupfer H., Siering C., Klenk H. P. 2009; Nocardiopsis potens sp. nov., isolated from household waste. Int J Syst Evol Microbiol 59:2729–2733 [CrossRef]
     [Google Scholar]
  51. Zhang Z., Wang Y., Ruan J. 1998; Reclassification of Thermomonospora and Microtetraspora . Int J Syst Bacteriol 48:411–422 [CrossRef]
     [Google Scholar]
  52. Zhang X., Zhang L.-P., Yang R., Shi N., Lu Z., Chen W.-X., Jiang C.-L., Yu L.-H. 2008; Nocardiopsis ganjiahuensis sp. nov., isolated from a soil from Ganjiahu, China. Int J Syst Evol Microbiol 58:195–199 [CrossRef]
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.015990-0
Loading
Murinocardiopsis flavida gen. nov., sp. nov., an actinomycete isolated from indoor walls
Int J Syst Evol Microbiol 60, 1729 (2010); https://doi.org/10.1099/ijs.0.015990-0
/content/journal/ijsem/10.1099/ijs.0.015990-0
/content/journal/ijsem/10.1099/ijs.0.015990-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error