@article{mbs:/content/journal/ijsem/10.1099/ijs.0.015990-0, author = "Kämpfer, P. and Schäfer, J. and Lodders, N. and Martin, K.", title = "Murinocardiopsis flavida gen. nov., sp. nov., an actinomycete isolated from indoor walls", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2010", volume = "60", number = "8", pages = "1729-1734", doi = "https://doi.org/10.1099/ijs.0.015990-0", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.015990-0", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", abstract = "Two Gram-stain-positive, mycelium-forming actinobacteria (strains 14-Be-013T and 02-Gi-014) were isolated from walls colonized with moulds and studied taxonomically. The isolates formed yellowish-pigmented substrate mycelium showing no fragmentation. Comparative analysis of 16S rRNA gene sequences showed that these bacteria are most closely related to genera within the family Nocardiopsaceae, but form a separate lineage within this family. Highest sequence similarities were to the type strains of Marinactinospora thermotolerans (96.0 % to 14-Be-013T), Nocardiopsis dassonvillei subsp. albirubida and Nocardiopsis lucentensis (both 95.3 % to 14-Be-013T). Whole-cell hydrolysates contained meso-diaminopimelic acid as the diagnostic diamino acid of the cell wall and no diagnostic sugars. Mycolic acids were absent. The major menaquinones were MK-10(H4), MK-11(H4) and MK-12(H2). The polar lipid profile consisted of phosphatidylcholine, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and unknown lipids. Major fatty acids iso-C16 : 0, anteiso-C17 : 0 and C18 : 1 ω9c supported the affiliation of these isolates to the family Nocardiopsaceae. Phenotypic analysis (including chemotaxonomy) further differentiated strains 14-Be-013T and 02-Gi-014 from the most closely related members of the genera Marinactinospora and Nocardiopsis. Since the two strains form a distinct lineage in the 16S rRNA gene sequence-based phylogenetic tree, the novel genus Murinocardiopsis gen. nov. with the type species Murinocardiopsis flavida sp. nov. is proposed. The type strain of Murinocardiopsis flavida is 14-Be-013T (=DSM 45312T =CCM 7612T).", }