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Abstract

Two novel actinomycetes, designated DQS3-9A1 and DQS3-9A2, were isolated from a saline soil contaminated with crude oil in the Shengli Oilfield in China. On the basis of 16S rRNA gene sequence analysis, the two strains were most closely related to species (92.7–94.9 % similarities), and formed a distinct lineage in the suborder . In addition, the major sugars in the cell wall, arabinose and galactose, supported the affiliation of strain DQS3-9A1 with members of the family . However, strain DQS3-9A1 did not contain mycolic acids and MK-8 (85.5 %) was the major menaquinone for both isolates. The major cellular fatty acids for strain DQS3-9A1 were C (20.5 %), 10-methyl C (19.3 %), 10-methyl C (16.1 %), summed feature 3 (11.4 %), C (11.3 %), C (5.0 %) and C 8 (5.0 %). The polar lipids of strain DQS3-9A1 consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, phosphatidylinositol and an unknown glucosamine-containing phospholipid. These chemotaxonomic data indicated that strain DQS3-9A1 differs from the present members of the suborder . Therefore, the creation of gen. nov., sp. nov. is proposed, with DQS3-9A1 (=DSM 45089=CGMCC 4.3532) as the type strain.

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2010-03-01
2019-12-05
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References

  1. Butler, W. R., Floyd, M. M., Brown, J., Toney, S. R., Daneshvar,M., Cooksey, R. S., Carr, J., Steigerwalt, A. & Charles, N. ( 2005; ). Novel mycolic acid-containing bacteria in the family Segniliparaceae fam. nov., including the genus Segniliparusgen. nov., with descriptions of Segniliparus rotundus sp. nov. and Segniliparus rugosus sp. nov. Int J Syst Evol Microbiol 55, 1615–1624.[CrossRef]
    [Google Scholar]
  2. Chun, J., Blackall, L. L., Kang, S. O., Hah, Y. C. & Goodfellow,M. ( 1997; ). A proposal to reclassify Nocardia pinensis Blackall et al. as Skermania piniformis gen. nov., comb. nov. Int J Syst Bacteriol 47, 127–131.[CrossRef]
    [Google Scholar]
  3. Collins, M. D., Goodfellow, M. & Minnikin, D. E. ( 1980; ). Fatty acid isoprenoid quinine and polar lipid compositionin the classification of Curtobacterium and related taxa. J Gen Microbiol 118, 29–37.
    [Google Scholar]
  4. Collins, M. D., Burton, R. A. & Jones, D. ( 1988; ). Corynebacterium amycolatum sp. nov., a new mycolicacid-less Corynebacterium species from human skin. FEMSMicrobiol Lett 49, 349–352.
    [Google Scholar]
  5. Collins, M. D., Falsen, E., Akervall, E., Sjöden, B. &Alvarez, A. ( 1998; ). Corynebacterium kroppenstedtii sp. nov., a novel corynebacterium that does not contain mycolic acids. Int J Syst Bacteriol 48, 1449–1454.[CrossRef]
    [Google Scholar]
  6. Collins, M. D., Hoyles, L., Foster, G. & Falsen, E. ( 2004; ). Corynebacterium caspium sp. nov., from aCaspian seal (Phoca caspica). Int J Syst EvolMicrobiol 54, 925–928.
    [Google Scholar]
  7. De Ley, J., Cattoir, H. & Reynaerts, A. ( 1970; ). The quantitative measurement of DNA hybridization from renaturationrates. Eur J Biochem 12, 133–142.[CrossRef]
    [Google Scholar]
  8. Eguchi, M., Nishikawa, T. & Macdonald, K. ( 1996; ). Responses to stress and nutrient availability by the marineultramicrobacterium Sphingomonas sp. strain RB2256. ApplEnviron Microbiol 62, 1287–1294.
    [Google Scholar]
  9. Felsenstein, J. ( 1985; ). Confidence limitson phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef]
    [Google Scholar]
  10. Fernández-Garayzábal, J. F., Vela, A. I., Egido,R., Hutson, R. A., Lanzarot, M. P., Fernández-García, M. &Collins, M. D. ( 2004; ). Corynebacterium ciconiae sp. nov., isolated from the trachea of black storks (Ciconianigra). Int J Syst Evol Microbiol 54, 2191–2195.[CrossRef]
    [Google Scholar]
  11. Hall, V., Collins, M. D., Hutson, R. A., Lawson, P. A., Falsen,E. & Duerden, B. I. ( 2003; ). Corynebacteriumatypicum sp. nov., from a human clinical source, does not contain corynomycolicacids. Int J Syst Evol Microbiol 53, 1065–1068.[CrossRef]
    [Google Scholar]
  12. Huß, V. A. R., Festl, H. & Schleifer, K.-H. ( 1983; ). Studies on the spectrometric determination of DNAhybridisation from renaturation rates. Syst Appl Microbiol 4, 184–192.[CrossRef]
    [Google Scholar]
  13. Kämpfer, P., Anderson, M. A., Rainey, F. A., Kroppenstedt,R. M. & Salonen, S. M. ( 1999; ). Williamsiamuralis gen. nov., sp. nov., isolated from the indoor environment ofa children's day care centre. Int J Syst Bacteriol 49, 681–687.[CrossRef]
    [Google Scholar]
  14. Kates, M. ( 1986; ). Techniques ofLipidology, 2nd edn. Amsterdam: Elsevier.
  15. Kimura, M. ( 1980; ). A simple method forestimating evolutionary rates of base substitutions through comparative studiesof nucleotide sequences. J Mol Evol 16, 111–120.[CrossRef]
    [Google Scholar]
  16. Komagata, K. & Suzuki, K. ( 1987; ).Lipid and cell wall analysis in bacterial systematics. MethodsMicrobiol 19, 161–206.
    [Google Scholar]
  17. Kumar, S., Tamura, K. & Nei, M. ( 2004; ). mega3: integrated software for molecular evolutionary geneticsanalysis and sequence alignment. Brief Bioinform 5, 150–163.[CrossRef]
    [Google Scholar]
  18. Li, W. L. ( 2007; ). Corynebacterineae.In Actinomycete Systematic Principles, Methods and Practice, pp.323–358. Edited by L. H. Xu, W. L. Li, Z. H. Liu & C. L. Jiang.Beijing: Science Press.
  19. Marmur, J. & Doty, P. ( 1962; ). Determinationof the base composition of deoxyribonucleic acid from its thermal denaturationtemperature. J Mol Biol 5, 109–118.[CrossRef]
    [Google Scholar]
  20. Rainey, F. A., Klatte, S., Kroppenstedt, R. M. & Stackebrandt,E. ( 1995; ). Dietzia, a new genus including Dietzia maris comb. nov., formerly Rhodococcus maris. Int J Syst Bacteriol 45, 32–36.[CrossRef]
    [Google Scholar]
  21. Saitou, N. & Nei, M. ( 1987; ). Theneighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  22. Soddell, J. A., Stainsby, F. M., Eales, K. L., Kroppenstedt,R. M., Robert, J., Seviour, R. J. & Goodfellow, M. ( 2006; ). Millisia brevis gen. nov., sp. nov., an actinomyceteisolated from activated sludge foam. Int J Syst Evol Microbiol 56, 739–744.[CrossRef]
    [Google Scholar]
  23. Stackebrandt, E., Rainey, F. A. & Ward-Rainey, N. L. ( 1997; ). Proposal for a new hierarchic classification system, Actinobacteria classis nov. Int J Syst Bacteriol 47, 479–491.[CrossRef]
    [Google Scholar]
  24. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. &Higgins, D. G. ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignmentaided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef]
    [Google Scholar]
  25. Wang, Y. N., Cai, H., Yu, S. L., Wang, Z. Y., Liu, J. &Wu, X. L. ( 2007; ). Halomonas gudaonensis sp.nov., isolated from a saline soil contaminated by crude oil. IntJ Syst Evol Microbiol 57, 911–915.
    [Google Scholar]
  26. Wauters, G., Driessen, A., Ageron, E., Janssens, M. & Grimont,P. A. D. ( 1996; ). Propionic acid-producing strainspreviously designated as Corynebacterium xerosis, C. minutissimum, C. striatum, and CDC group I and group F coryneforms belongto the species Corynebacterium amycolatum. Int J SystBacteriol 46, 653–657.
    [Google Scholar]
  27. Williams, S. T., Goodfellow, M., Alderson, G., Wellington, E.M. H., Sneath, P. H. A. & Sackin, M. J. ( 1983; ).Numerical classification of Streptomyces and related genera. J Gen Microbiol 129, 1743–1813.
    [Google Scholar]
  28. Zumft, W. G. ( 1991; ). The denitrifyingbacteria. In The Prokaryotes. A Handbook on the Biology of Bacteria: Ecophysiology,Isolation, Identification, Application, 2nd edn, pp. 554–582. Editedby A. Balows, H. G. Trüper, M. Dworkin, W. Harder & K. H. Schleifer.New York: Springer.
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