@article{mbs:/content/journal/ijsem/10.1099/ijs.0.010546-0, author = "Wang, Ya-Nan and Chi, Chang-Qiao and Cai, Man and Lou, Zhi-Ying and Tang, Yue-Qin and Zhi, Xiao-Yang and Li, Wen-Jun and Wu, Xiao-Lei and Du, Xun", title = "Amycolicicoccus subflavus gen. nov., sp. nov., an actinomycete isolated from a saline soil contaminated by crude oil", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2010", volume = "60", number = "3", pages = "638-643", doi = "https://doi.org/10.1099/ijs.0.010546-0", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.010546-0", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", abstract = "Two novel actinomycetes, designated DQS3-9A1T and DQS3-9A2, were isolated from a saline soil contaminated with crude oil in the Shengli Oilfield in China. On the basis of 16S rRNA gene sequence analysis, the two strains were most closely related to Mycobacterium species (92.7–94.9 % similarities), and formed a distinct lineage in the suborder Corynebacterineae. In addition, the major sugars in the cell wall, arabinose and galactose, supported the affiliation of strain DQS3-9A1T with members of the family Mycobacteriaceae. However, strain DQS3-9A1T did not contain mycolic acids and MK-8 (85.5 %) was the major menaquinone for both isolates. The major cellular fatty acids for strain DQS3-9A1T were C16 : 0 (20.5 %), 10-methyl C17 : 0 (19.3 %), 10-methyl C18 : 0 (16.1 %), summed feature 3 (11.4 %), C15 : 0 (11.3 %), C17 : 0 (5.0 %) and C17 : 1 ω8c (5.0 %). The polar lipids of strain DQS3-9A1T consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, phosphatidylinositol and an unknown glucosamine-containing phospholipid. These chemotaxonomic data indicated that strain DQS3-9A1T differs from the present members of the suborder Corynebacterineae. Therefore, the creation of Amycolicicoccus subflavus gen. nov., sp. nov. is proposed, with DQS3-9A1T (=DSM 45089T=CGMCC 4.3532T) as the type strain.", }