1887

Abstract

A novel betaproteobacterium, strain TBEA3, was isolated from soil using enrichment cultures with the organic thioether 3,3′-thiodipropionic acid as sole carbon and energy source. Analysis of the 16S rRNA gene sequence revealed 99.1 % sequence similarities to a poorly characterized member of the family , strain SB1, which had been previously isolated from activated sludge. Both strains showed highest gene sequence similarities (up to 96.9 %) to members of the genera and . The DNA G+C contents of strains TBEA3 and SB1 were 69.1 and 70.1 mol%, respectively, and the DNA–DNA hybridization value between these two strains was 45.3 %. The predominant cellular fatty acids in both strains were C, C 7 and summed feature 3 (C 7 and/or C iso 2-OH). The major 3-hydroxy fatty acid was C 3-OH. Based on the genetic and chemotaxonomic data, strains TBEA3 and SB1 represent two novel species of a new genus within the family , for which the name gen. nov. is proposed. Strain TBEA3 (=LMG 24555=DSM 21634) is assigned to sp. nov., as the type strain of the type species of the genus. Strain SB1 (=LMG 24543=DSM 21598) is the type strain of sp. nov.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.006791-0
2009-11-01
2019-10-19
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/59/11/2702.html?itemId=/content/journal/ijsem/10.1099/ijs.0.006791-0&mimeType=html&fmt=ahah

References

  1. Appel, R. B., Tomlinson, I. A. & Hill, I. ( 1995; ). New reagents for the reductive quenching of ozonolysis reactions. Synth Commun 25, 3589–3595.[CrossRef]
    [Google Scholar]
  2. Bathe, S. ( 2004; ). Conjugal transfer of plasmid pNB2 to activated sludge bacteria leads to 3-chloroaniline degradation in enrichment cultures. Lett Appl Microbiol 38, 527–531.[CrossRef]
    [Google Scholar]
  3. Blenden, D. C. & Goldberg, H. S. ( 1965; ). Silver impregnation stain for Leptospira and flagella. J Bacteriol 89, 899–900.
    [Google Scholar]
  4. Bradford, M. M. ( 1976; ). A rapid and sensitive method for quantitation of micrograms quantities of proteins utilizing the principle of protein dye binding. Anal Biochem 72, 248–254.[CrossRef]
    [Google Scholar]
  5. Brandl, H., Gross, A., Lenz, R. W. & Fuller, R. C. ( 1988; ). Pseudomonas oleovorans as a source of poly(β-hydroxyalkanoates) for potential applications as biodegradable polyesters. Appl Environ Microbiol 54, 1977–1982.
    [Google Scholar]
  6. Bruland, N., Wübbeler, J. H. & Steinbüchel, A. ( 2009; ). 3-Mercaptopropionate dioxygenase, a cysteine dioxygenase homologue, catalyzes the initial step of 3-mercaptopropionate catabolism in the 3,3′-thiodipropionic acid degrading bacterium Variovorax paradoxus. J Biol Chem 284, 660–672.[CrossRef]
    [Google Scholar]
  7. Ding, L. & Yokota, A. ( 2004; ). Proposal of Curvibacter gracilis gen. nov., sp. nov. and Herbaspirillium putei sp. nov. for bacterial strains isolated from well water and reclassification of [Pseudomonas] huttiensis, [Pseudomonas] lanceolata, [Aquaspirillium] delicatum and [Aquaspirillium] autotrophicum as Herbaspirillium huttiense comb. nov., Curvibacter lanceolatus comb. nov., Curvibacter delicatus comb. nov. and Herbaspirillium autotrophicum comb. nov. Int J Syst Evol Microbiol 54, 2223–2230.[CrossRef]
    [Google Scholar]
  8. Felsenstein, J. ( 2002; ). phylip (phylogeny inference package), version 3.68. Distributed by the author. Department of Genome Sciences, University of Washington, Seattle, USA.
  9. Finneran, K. T., Johnsen, C. V. & Lovley, D. R. ( 2003; ). Rhodoferax ferrireducens sp. nov., a psychrotolerant, facultatively anaerobic bacterium that oxidizes acetate with the reduction of Fe(III). Int J Syst Evol Microbiol 53, 669–673.[CrossRef]
    [Google Scholar]
  10. Gerhardt, P., Murray, R. G. E., Wood, W. A. & Krieg, N. R. ( 1994; ). Methods for General and Molecular Bacteriology. Washington, DC: American Society for Microbiology.
  11. Goris, J., Suzuki, K.-I., De Vos, P., Nakase, T. & Kersters, K. ( 1998; ). Evaluation of a microplate DNA-DNA hybridisation method compared with the initial renaturation method. Can J Microbiol 44, 1148–1153.[CrossRef]
    [Google Scholar]
  12. Hiraishi, A. & Imhoff, F. J. ( 2005; ). Genus IX. Rhodoferax. In Bergey's Manual of Systematic Bacteriology, 2nd edn, vol. 2, pp. 727–731. Edited by D. J. Brenner, N. R. Krieg, J. T. Staley & G. M. Garrity. New York: Springer.
  13. Hiraishi, A., Hoshino, Y. & Satoh, T. ( 1991; ). Rhodoferax fermentans gen. nov., sp. nov., a phototrophic purple nonsulfur bacterium previously referred to as the “Rhodocyclus gelatinosus-like” group. Arch Microbiol 155, 330–336.
    [Google Scholar]
  14. Lehman, A. J., Fitzhugh, O. G., Nelson, A. A. & Woodward, G. ( 1951; ). The pharmacological evaluation of antioxidants. Adv Food Res 3, 197–208.
    [Google Scholar]
  15. Lütke-Eversloh, T. & Steinbüchel, A. ( 2003; ). Novel precursor substrates for polythioesters (PTE) and limits of PTE biosynthesis in Ralstonia eutropha. FEMS Microbiol Lett 221, 191–196.[CrossRef]
    [Google Scholar]
  16. Lütke-Eversloh, T. & Steinbüchel, A. ( 2004; ). Microbial polythioesters. Macromol Biosci 4, 166–174.
    [Google Scholar]
  17. Madigan, M. T., Jung, D. O., Woese, C. R. & Achenbach, L. A. ( 2000; ). Rhodoferax antarcticus sp. nov., a moderately psychrophilic purple non-sulphur bacterium isolated from an Antarctic microbial mat. Arch Microbiol 173, 269–277.[CrossRef]
    [Google Scholar]
  18. Maidak, B. L., Olsen, G. J., Larsen, N., Overbeek, R., McCaughey, M. J. & Woese, C. R. ( 1997; ). The RDP (Ribosomal Database Project). Nucleic Acids Res 25, 109–111.[CrossRef]
    [Google Scholar]
  19. Marmur, J. ( 1961; ). A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3, 208–218.[CrossRef]
    [Google Scholar]
  20. Mesbah, M., Premachandran, U. & Whitman, W. B. ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 38, 159–167.
    [Google Scholar]
  21. Mohn, W. W. ( 1995; ). Bacteria obtained from a sequencing batch reactor that are capable of growth on dehydroabietic acid. Appl Environ Microbiol 61, 2145–2150.
    [Google Scholar]
  22. Page, R. D. M. ( 1996; ). TreeView: an application to display phylogenetic trees on personal computers. Comput Appl Biosci 12, 357–358.
    [Google Scholar]
  23. Pot, B. & Gillis, M. ( 2005; ). Genus III. Aquaspirillium. In Bergey's Manual of Systematic Bacteriology, 2nd edn, vol. 2, pp. 801–823. Edited by D. J. Brenner, N. R. Krieg, J. T. Staley & G. M. Garrity. New York: Springer.
  24. Ryu, S. H., Lee, D. S., Park, M., Wang, Q., Jang, H. H., Park, W. & Jeon, C. O. ( 2008; ). Caenimonas koreensis gen. nov., sp. nov., isolated from activated sludge. Int J Syst Evol Microbiol 58, 1064–1068.[CrossRef]
    [Google Scholar]
  25. Schlegel, H. G., Kaltwasser, H. & Gottschalk, G. ( 1961; ). A submersion method for culture of hydrogen-oxidizing bacteria: growth physiological studies. Arch Mikrobiol 38, 209–222 (in German).[CrossRef]
    [Google Scholar]
  26. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G. ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef]
    [Google Scholar]
  27. Timm, A., Byrom, D. & Steinbüchel, A. ( 1990; ). Formation of blends of various poly(3-hydroxyalkanoic acids) by a recombinant strain of Pseudomonas oleovorans. Appl Microbiol Biotechnol 33, 296–301.[CrossRef]
    [Google Scholar]
  28. Vandamme, P., Vancanneyt, M., Pot, B., Mels, L., Hoste, B., Dewettinck, D., Vlaes, L., Van Den Borre, C., Higgins, R. & other authors ( 1992; ). Polyphasic taxonomic study of the emended genus Arcobacter with Arcobacter butzleri comb. nov. and Arcobacter skirrowii sp. nov., an aerotolerant bacterium isolated from veterinary specimens. Int J Syst Bacteriol 42, 344–356.[CrossRef]
    [Google Scholar]
  29. Weber, N., Klein, E. & Vosmann, K. ( 2006; ). Dialkyl 3,3′-thiodipropionate and dialkyl 2,2′ thiodiacetate antioxidants by lipase-catalyzed esterification and transesterifications. J Agric Food Chem 54, 2957–2963.[CrossRef]
    [Google Scholar]
  30. Willems, A. & Gillis, M. ( 2005; ). Family IV. Comamonadaceae. In Bergey's Manual of Systematic Bacteriology, 2nd edn, vol. 2, pp. 686–759. Edited by D. J. Brenner, N. R. Krieg, J. T. Staley & G. M. Garrity. New York: Springer.
  31. Wübbeler, J. H., Lütke-Eversloh, T., Van Trappen, S., Vandamme, P. & Steinbüchel, A. ( 2006; ). Tetrathiobacter mimigardefordensis sp. nov., isolated from compost, a betaproteobacterium capable of utilizing the organic disulfide 3,3′-dithiodipropionic acid. Int J Syst Evol Microbiol 56, 1305–1310.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.006791-0
Loading
/content/journal/ijsem/10.1099/ijs.0.006791-0
Loading

Data & Media loading...

Supplements

Structure of thiodipropionic acid (TDP).

IMAGE

[ Combined PDF file] 74 KB

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error