A novel betaproteobacterium, strain TBEA3, was isolated from soil using enrichment cultures with the organic thioether 3,3′-thiodipropionic acid as sole carbon and energy source. Analysis of the 16S rRNA gene sequence revealed 99.1 % sequence similarities to a poorly characterized member of the family , strain SB1, which had been previously isolated from activated sludge. Both strains showed highest gene sequence similarities (up to 96.9 %) to members of the genera and . The DNA G+C contents of strains TBEA3 and SB1 were 69.1 and 70.1 mol%, respectively, and the DNA–DNA hybridization value between these two strains was 45.3 %. The predominant cellular fatty acids in both strains were C, C 7 and summed feature 3 (C 7 and/or C iso 2-OH). The major 3-hydroxy fatty acid was C 3-OH. Based on the genetic and chemotaxonomic data, strains TBEA3 and SB1 represent two novel species of a new genus within the family , for which the name gen. nov. is proposed. Strain TBEA3 (=LMG 24555=DSM 21634) is assigned to sp. nov., as the type strain of the type species of the genus. Strain SB1 (=LMG 24543=DSM 21598) is the type strain of sp. nov.


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Structure of thiodipropionic acid (TDP).


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