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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 59, Issue 11

gen. nov., sp. nov. and sp. nov., two members of the class belonging to the family Free

Abstract

A novel betaproteobacterium, strain TBEA3, was isolated from soil using enrichment cultures with the organic thioether 3,3′-thiodipropionic acid as sole carbon and energy source. Analysis of the 16S rRNA gene sequence revealed 99.1 % sequence similarities to a poorly characterized member of the family , strain SB1, which had been previously isolated from activated sludge. Both strains showed highest gene sequence similarities (up to 96.9 %) to members of the genera and . The DNA G+C contents of strains TBEA3 and SB1 were 69.1 and 70.1 mol%, respectively, and the DNA–DNA hybridization value between these two strains was 45.3 %. The predominant cellular fatty acids in both strains were C, C 7 and summed feature 3 (C 7 and/or C iso 2-OH). The major 3-hydroxy fatty acid was C 3-OH. Based on the genetic and chemotaxonomic data, strains TBEA3 and SB1 represent two novel species of a new genus within the family , for which the name gen. nov. is proposed. Strain TBEA3 (=LMG 24555=DSM 21634) is assigned to sp. nov., as the type strain of the type species of the genus. Strain SB1 (=LMG 24543=DSM 21598) is the type strain of sp. nov.

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Keyword(s): PTE, polythioester and TDP, 3,3′-thiodipropionic acid
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2009-11-01
2025-05-19
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References

  1. Appel, R. B., Tomlinson, I. A. & Hill, I.(1995). New reagents for the reductive quenching of ozonolysis reactions. Synth Commun 25, 3589–3595.[CrossRef] [Google Scholar]
  2. Bathe, S.(2004). Conjugal transfer of plasmid pNB2 to activated sludge bacteria leads to 3-chloroaniline degradation in enrichment cultures. Lett Appl Microbiol 38, 527–531.[CrossRef] [Google Scholar]
  3. Blenden, D. C. & Goldberg, H. S.(1965). Silver impregnation stain for Leptospira and flagella. J Bacteriol 89, 899–900. [Google Scholar]
  4. Bradford, M. M.(1976). A rapid and sensitive method for quantitation of micrograms quantities of proteins utilizing the principle of protein dye binding. Anal Biochem 72, 248–254.[CrossRef] [Google Scholar]
  5. Brandl, H., Gross, A., Lenz, R. W. & Fuller, R. C.(1988).Pseudomonas oleovorans as a source of poly(β-hydroxyalkanoates) for potential applications as biodegradable polyesters. Appl Environ Microbiol 54, 1977–1982. [Google Scholar]
  6. Bruland, N., Wübbeler, J. H. & Steinbüchel, A.(2009). 3-Mercaptopropionate dioxygenase, a cysteine dioxygenase homologue, catalyzes the initial step of 3-mercaptopropionate catabolism in the 3,3′-thiodipropionic acid degrading bacterium Variovorax paradoxus. J Biol Chem 284, 660–672.[CrossRef] [Google Scholar]
  7. Ding, L. & Yokota, A.(2004). Proposal of Curvibacter gracilis gen. nov., sp. nov. and Herbaspirillium putei sp. nov. for bacterial strains isolated from well water and reclassification of [Pseudomonas] huttiensis, [Pseudomonas] lanceolata, [Aquaspirillium] delicatum and [Aquaspirillium] autotrophicum as Herbaspirillium huttiense comb. nov., Curvibacter lanceolatus comb. nov., Curvibacter delicatus comb. nov. and Herbaspirillium autotrophicum comb. nov. Int J Syst Evol Microbiol 54, 2223–2230.[CrossRef] [Google Scholar]
  8. Felsenstein, J.(2002).phylip (phylogeny inference package), version 3.68. Distributed by the author. Department of Genome Sciences, University of Washington, Seattle, USA.
  9. Finneran, K. T., Johnsen, C. V. & Lovley, D. R.(2003).Rhodoferax ferrireducens sp. nov., a psychrotolerant, facultatively anaerobic bacterium that oxidizes acetate with the reduction of Fe(III). Int J Syst Evol Microbiol 53, 669–673.[CrossRef] [Google Scholar]
  10. Gerhardt, P., Murray, R. G. E., Wood, W. A. & Krieg, N. R.(1994).Methods for General and Molecular Bacteriology. Washington, DC: American Society for Microbiology.
  11. Goris, J., Suzuki, K.-I., De Vos, P., Nakase, T. & Kersters, K.(1998). Evaluation of a microplate DNA-DNA hybridisation method compared with the initial renaturation method. Can J Microbiol 44, 1148–1153.[CrossRef] [Google Scholar]
  12. Hiraishi, A. & Imhoff, F. J.(2005). Genus IX. Rhodoferax. In Bergey's Manual of Systematic Bacteriology, 2nd edn, vol. 2, pp. 727–731. Edited by D. J. Brenner, N. R. Krieg, J. T. Staley & G. M. Garrity. New York: Springer.
  13. Hiraishi, A., Hoshino, Y. & Satoh, T.(1991).Rhodoferax fermentans gen. nov., sp. nov., a phototrophic purple nonsulfur bacterium previously referred to as the “Rhodocyclus gelatinosus-like” group. Arch Microbiol 155, 330–336. [Google Scholar]
  14. Lehman, A. J., Fitzhugh, O. G., Nelson, A. A. & Woodward, G.(1951). The pharmacological evaluation of antioxidants. Adv Food Res 3, 197–208. [Google Scholar]
  15. Lütke-Eversloh, T. & Steinbüchel, A.(2003). Novel precursor substrates for polythioesters (PTE) and limits of PTE biosynthesis in Ralstonia eutropha. FEMS Microbiol Lett 221, 191–196.[CrossRef] [Google Scholar]
  16. Lütke-Eversloh, T. & Steinbüchel, A.(2004). Microbial polythioesters. Macromol Biosci 4, 166–174. [Google Scholar]
  17. Madigan, M. T., Jung, D. O., Woese, C. R. & Achenbach, L. A.(2000).Rhodoferax antarcticus sp. nov., a moderately psychrophilic purple non-sulphur bacterium isolated from an Antarctic microbial mat. Arch Microbiol 173, 269–277.[CrossRef] [Google Scholar]
  18. Maidak, B. L., Olsen, G. J., Larsen, N., Overbeek, R., McCaughey, M. J. & Woese, C. R.(1997). The RDP (Ribosomal Database Project). Nucleic Acids Res 25, 109–111.[CrossRef] [Google Scholar]
  19. Marmur, J.(1961). A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3, 208–218.[CrossRef] [Google Scholar]
  20. Mesbah, M., Premachandran, U. & Whitman, W. B.(1989). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 38, 159–167. [Google Scholar]
  21. Mohn, W. W.(1995). Bacteria obtained from a sequencing batch reactor that are capable of growth on dehydroabietic acid. Appl Environ Microbiol 61, 2145–2150. [Google Scholar]
  22. Page, R. D. M.(1996). TreeView: an application to display phylogenetic trees on personal computers. Comput Appl Biosci 12, 357–358. [Google Scholar]
  23. Pot, B. & Gillis, M.(2005). Genus III. Aquaspirillium. In Bergey's Manual of Systematic Bacteriology, 2nd edn, vol. 2, pp. 801–823. Edited by D. J. Brenner, N. R. Krieg, J. T. Staley & G. M. Garrity. New York: Springer.
  24. Ryu, S. H., Lee, D. S., Park, M., Wang, Q., Jang, H. H., Park, W. & Jeon, C. O.(2008).Caenimonas koreensis gen. nov., sp. nov., isolated from activated sludge. Int J Syst Evol Microbiol 58, 1064–1068.[CrossRef] [Google Scholar]
  25. Schlegel, H. G., Kaltwasser, H. & Gottschalk, G.(1961). A submersion method for culture of hydrogen-oxidizing bacteria: growth physiological studies. Arch Mikrobiol 38, 209–222 (in German).[CrossRef] [Google Scholar]
  26. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G.(1997). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef] [Google Scholar]
  27. Timm, A., Byrom, D. & Steinbüchel, A.(1990). Formation of blends of various poly(3-hydroxyalkanoic acids) by a recombinant strain of Pseudomonas oleovorans. Appl Microbiol Biotechnol 33, 296–301.[CrossRef] [Google Scholar]
  28. Vandamme, P., Vancanneyt, M., Pot, B., Mels, L., Hoste, B., Dewettinck, D., Vlaes, L., Van Den Borre, C., Higgins, R. & other authors(1992). Polyphasic taxonomic study of the emended genus Arcobacter with Arcobacter butzleri comb. nov. and Arcobacter skirrowii sp. nov., an aerotolerant bacterium isolated from veterinary specimens. Int J Syst Bacteriol 42, 344–356.[CrossRef] [Google Scholar]
  29. Weber, N., Klein, E. & Vosmann, K.(2006). Dialkyl 3,3′-thiodipropionate and dialkyl 2,2′ thiodiacetate antioxidants by lipase-catalyzed esterification and transesterifications. J Agric Food Chem 54, 2957–2963.[CrossRef] [Google Scholar]
  30. Willems, A. & Gillis, M.(2005). Family IV. Comamonadaceae. In Bergey's Manual of Systematic Bacteriology, 2nd edn, vol. 2, pp. 686–759. Edited by D. J. Brenner, N. R. Krieg, J. T. Staley & G. M. Garrity. New York: Springer.
  31. Wübbeler, J. H., Lütke-Eversloh, T., Van Trappen, S., Vandamme, P. & Steinbüchel, A.(2006).Tetrathiobacter mimigardefordensis sp. nov., isolated from compost, a betaproteobacterium capable of utilizing the organic disulfide 3,3′-dithiodipropionic acid. Int J Syst Evol Microbiol 56, 1305–1310.[CrossRef] [Google Scholar]
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Pseudorhodoferax soli gen. nov., sp. nov. and Pseudorhodoferax caeni sp. nov., two members of the class Betaproteobacteria belonging to the family Comamonadaceae
Int J Syst Evol Microbiol 59, 2702 (2009); https://doi.org/10.1099/ijs.0.006791-0
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Structure of thiodipropionic acid (TDP).

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