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A novel betaproteobacterium, strain TBEA3T, was isolated from soil using enrichment cultures with the organic thioether 3,3′-thiodipropionic acid as sole carbon and energy source. Analysis of the 16S rRNA gene sequence revealed 99.1 % sequence similarities to a poorly characterized member of the family Comamonadaceae, strain SB1T, which had been previously isolated from activated sludge. Both strains showed highest gene sequence similarities (up to 96.9 %) to members of the genera Rhodoferax and Curvibacter. The DNA G+C contents of strains TBEA3T and SB1T were 69.1 and 70.1 mol%, respectively, and the DNA–DNA hybridization value between these two strains was 45.3 %. The predominant cellular fatty acids in both strains were C16 : 0, C18 : 1 ω7c and summed feature 3 (C16 : 1 ω7c and/or C15 : 0 iso 2-OH). The major 3-hydroxy fatty acid was C10 : 0 3-OH. Based on the genetic and chemotaxonomic data, strains TBEA3T and SB1T represent two novel species of a new genus within the family Comamonadaceae, for which the name Pseudorhodoferax gen. nov. is proposed. Strain TBEA3T (=LMG 24555T=DSM 21634T) is assigned to Pseudorhodoferax soli sp. nov., as the type strain of the type species of the genus. Strain SB1T (=LMG 24543T=DSM 21598T) is the type strain of Pseudorhodoferax caeni sp. nov.
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