RT Journal Article SR Electronic(1) A1 Bruland, Nadine A1 Bathe, Stephan A1 Willems, Anne A1 Steinbüchel, AlexanderYR 2009 T1 Pseudorhodoferax soli gen. nov., sp. nov. and Pseudorhodoferax caeni sp. nov., two members of the class Betaproteobacteria belonging to the family Comamonadaceae JF International Journal of Systematic and Evolutionary Microbiology, VO 59 IS 11 SP 2702 OP 2707 DO https://doi.org/10.1099/ijs.0.006791-0 PB Microbiology Society, SN 1466-5034, AB A novel betaproteobacterium, strain TBEA3T, was isolated from soil using enrichment cultures with the organic thioether 3,3′-thiodipropionic acid as sole carbon and energy source. Analysis of the 16S rRNA gene sequence revealed 99.1 % sequence similarities to a poorly characterized member of the family Comamonadaceae, strain SB1T, which had been previously isolated from activated sludge. Both strains showed highest gene sequence similarities (up to 96.9 %) to members of the genera Rhodoferax and Curvibacter. The DNA G+C contents of strains TBEA3T and SB1T were 69.1 and 70.1 mol%, respectively, and the DNA–DNA hybridization value between these two strains was 45.3 %. The predominant cellular fatty acids in both strains were C16 : 0, C18 : 1 ω7c and summed feature 3 (C16 : 1 ω7c and/or C15 : 0 iso 2-OH). The major 3-hydroxy fatty acid was C10 : 0 3-OH. Based on the genetic and chemotaxonomic data, strains TBEA3T and SB1T represent two novel species of a new genus within the family Comamonadaceae, for which the name Pseudorhodoferax gen. nov. is proposed. Strain TBEA3T (=LMG 24555T=DSM 21634T) is assigned to Pseudorhodoferax soli sp. nov., as the type strain of the type species of the genus. Strain SB1T (=LMG 24543T=DSM 21598T) is the type strain of Pseudorhodoferax caeni sp. nov., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.006791-0