Nine Gram-negative, rod-shaped, non-spore-forming isolates with identical or very similar repetitive-sequence-based PCR profiles were recovered from an evaporative lagoon in Mexico. Two strains, designated 1N and 3N, had virtually identical 16S rRNA gene sequences and, on the basis of these sequences, were identified as members of the genus , with R-20805 as the closest relative. All nine isolates had practically identical whole-cell protein profiles. The major fatty acids [C C 7 and summed feature a (C 7 and/or C 6)] of strains 1N and 3N supported their affiliation with the genus . The DNA–DNA reassociation values with respect to LMG 23201 and other closely related species were <15 %. Physiological and biochemical tests allowed phenotypic differentiation of the strains analysed, including strain 1N, from the five phylogenetically closest species. On the basis of the data obtained by using this polyphasic taxonomic approach, the nine strains represent a novel species, for which the name sp. nov. is proposed. The type strain is 1N (=LMG 24676=CIP 109853).


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vol. , part 6, pp. 1416 – 1420

Pulsed-field electrophoresis gel of representative strains of sp. nov. and type and reference strains of known species [ PDF] (104 KB)

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