%0 Journal Article %A Escalante, Ana E. %A Caballero-Mellado, Jesús %A Martínez-Aguilar, Lourdes %A Rodríguez-Verdugo, Alejandra %A González-González, Andrea %A Toribio-Jiménez, Jeiry %A Souza, Valeria %T Pseudomonas cuatrocienegasensis sp. nov., isolated from an evaporating lagoon in the Cuatro Ciénegas valley in Coahuila, Mexico %D 2009 %J International Journal of Systematic and Evolutionary Microbiology, %V 59 %N 6 %P 1416-1420 %@ 1466-5034 %R https://doi.org/10.1099/ijs.0.006189-0 %I Microbiology Society, %X Nine Gram-negative, rod-shaped, non-spore-forming isolates with identical or very similar repetitive-sequence-based PCR profiles were recovered from an evaporative lagoon in Mexico. Two strains, designated 1NT and 3N, had virtually identical 16S rRNA gene sequences and, on the basis of these sequences, were identified as members of the genus Pseudomonas, with Pseudomonas peli R-20805T as the closest relative. All nine isolates had practically identical whole-cell protein profiles. The major fatty acids [C16 : 0, C18 : 1 ω7c and summed feature a (C16 : 1 ω7 and/or C16 : 1 ω6c)] of strains 1NT and 3N supported their affiliation with the genus Pseudomonas. The DNA–DNA reassociation values with respect to P. peli LMG 23201T and other closely related Pseudomonas species were <15 %. Physiological and biochemical tests allowed phenotypic differentiation of the strains analysed, including strain 1NT, from the five phylogenetically closest Pseudomonas species. On the basis of the data obtained by using this polyphasic taxonomic approach, the nine strains represent a novel species, for which the name Pseudomonas cuatrocienegasensis sp. nov. is proposed. The type strain is 1NT (=LMG 24676T=CIP 109853T). %U https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.006189-0