1887

Abstract

Two luminous marine bacterial strains, LC2-005 and LC2-102, were isolated from seawater at Kuroshio Region and Sagami Bay in Japan, respectively. These bacteria were Gram-negative, oxidase-positive, catalase-positive, motile and rod-shaped. On the basis of 16S rRNA gene sequence analysis, strains LC2-005 and LC2-102 formed a cluster within the species group. However, multilocus sequence analysis using five loci (, , , and ) and DNA–DNA hybridization experiments indicated that these strains were distinct from the currently known species. Additionally, these strains differ from related species in utilization of glucose, mannitol, inositol, sorbitol, rhamnose, sucrose, melibiose and arabinose, production of lysine decarboxylase, ornithine decarboxylase, tryptophan deaminase, esterase (C4), lipase (C4), chymotrypsin, acid phosphatase, -glucosidase, -glucosidase and -acetyl--glucosaminidase and the ability to reduce nitrate to nitrite. The major fatty acids were C iso 2-OH and/or C 7, C, C 7 and C. The DNA G+C contents of strains LC2-005 and LC2-102 were 45.2 and 45.5 mol%, respectively. On the basis of the polyphasic taxonomic evidence presented in this study, it can be concluded that strains LC2-005 and LC2-102 belong to the same genospecies and represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is LC2-005 (=NBRC 104587 =KCTC 22352).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.004283-0
2009-07-01
2019-10-19
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/59/7/1645.html?itemId=/content/journal/ijsem/10.1099/ijs.0.004283-0&mimeType=html&fmt=ahah

References

  1. Baumann, P. & Baumann, L. ( 1984; ). Genus II. Photobacterium Beijerinck 1889, 401AL. In Bergey's Manual of Systematic Bacteriology, vol. 1, pp. 539–545. Edited by N. R. Krieg & J. G. Holt. Baltimore, MD: Williams & Wilkins.
  2. Baumann, P. & Schubert, R. H. W. ( 1984; ). Family II. Vibrionaceae Veron 1965, 5245AL. In Bergey's Manual of Systematic Bacteriology, vol. 1, pp. 516–517. Edited by N. R. Krieg & J. G. Holt. Baltimore, MD: Williams & Wilkins.
  3. Ezaki, T., Hashimoto, Y. & Yabuuchi, E. ( 1989; ). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39, 224–229.[CrossRef]
    [Google Scholar]
  4. Farmer, J. J., III, Janda, J. M., Brenner, F. W., Cameron, D. N. & Birkhead, K. M. ( 2005; ). Genus I. Vibrio Pacini 1854, 411AL. In Bergey's Manual of Systematic Bacteriology, 2nd edn, vol. 2 part B, pp. 494–546. Edited by D. J. Brenner, N. R. Krieg & J. T. Staley. New York: Springer.
  5. Felsenstein, J. ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef]
    [Google Scholar]
  6. Gomez-Gil, B., Soto-Rodriguez, S., Garcia-Gasca, A., Roque, A., Vazquez-Juarez, R., Thompson, F. L. & Swings, J. ( 2004; ). Molecular identification of Vibrio harveyi-related isolates associated with diseased aquatic organisms. Microbiology 150, 1769–1777.[CrossRef]
    [Google Scholar]
  7. Kimura, M. ( 1980; ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide-sequences. J Mol Evol 16, 111–120.[CrossRef]
    [Google Scholar]
  8. Kumar, S., Tamura, K. & Nei, M. ( 2004; ). mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5, 150–163.[CrossRef]
    [Google Scholar]
  9. Lane, D. J. ( 1991; ). 16S/23S rRNA sequencing. In Nucleic Acid Techniques in Bacterial Systematics, pp. 115–175. Edited by E. Stackebrandt & M. Goodfellow. Chichester, UK: Wiley.
  10. Marmur, J. ( 1961; ). A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3, 208–218.[CrossRef]
    [Google Scholar]
  11. Mellado, E., Moore, E. R. B., Nieto, J. J. & Ventosa, A. ( 1996; ). Analysis of 16S rRNA gene sequences of Vibrio costicola strains: description of Salinivibrio costicola gen. nov., comb. nov. Int J Syst Bacteriol 46, 817–821.[CrossRef]
    [Google Scholar]
  12. Mesbah, M., Premachandran, U. & Whitman, W. B. ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef]
    [Google Scholar]
  13. Nealson, K. H., Wimpee, B. & Wimpee, C. ( 1993; ). Identification of Vibrio splendidus as a member of the planktonic luminous bacteria from the persian gulf and Kuwait region with luxa probes. Appl Environ Microbiol 59, 2684–2689.
    [Google Scholar]
  14. Nishino, T., Ikemoto, E. & Kogure, K. ( 2004; ). Application of atomic force microscopy to observation of marine bacteria. J Oceanogr 60, 219–225.[CrossRef]
    [Google Scholar]
  15. Oliver, J. D., Roberts, D. M., White, V. K., Dry, M. A. & Simpson, L. M. ( 1986; ). Bioluminescence in a strain of the human pathogenic bacterium Vibrio vulnificus. Appl Environ Microbiol 52, 1209–1211.
    [Google Scholar]
  16. Palmer, L. M. & Colwell, R. R. ( 1991; ). Detection of luciferase gene sequence in nonluminescent Vibrio cholerae by colony hybridization and polymerase chain reaction. Appl Environ Microbiol 57, 1286–1293.
    [Google Scholar]
  17. Pujalte, M.-J. & Garay, E. ( 1986; ). Proposal of Vibrio mediterranei sp. nov.: a new marine member of the genus Vibrio. Int J Syst Bacteriol 36, 278–281.[CrossRef]
    [Google Scholar]
  18. Reichelt, J. L. & Baumann, P. ( 1973; ). Taxonomy of the marine, luminous bacteria. Arch Mikrobiol 94, 283–330.[CrossRef]
    [Google Scholar]
  19. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  20. Sawabe, T., Kita-Tsukamoto, K. & Thompson, F. L. ( 2007; ). Inferring the evolutionary history of vibrios by means of multilocus sequence analysis. J Bacteriol 189, 7932–7936.[CrossRef]
    [Google Scholar]
  21. Thompson, J. D., Higgins, D. G. & Gibson, T. J. ( 1994; ). clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22, 4673–4680.[CrossRef]
    [Google Scholar]
  22. Thompson, F. L., Hoste, B., Thompson, C. C., Goris, J., Gomez-Gil, B., Huys, L., De Vos, P. & Swings, J. ( 2002; ). Enterovibrio norvegicus gen. nov., sp nov., isolated from the gut of turbot (Scophthalmus maximus) larvae: a new member of the family Vibrionaceae. Int J Syst Evol Microbiol 52, 2015–2022.[CrossRef]
    [Google Scholar]
  23. Thompson, F. L., Hoste, B., Vandemeulebroecke, K. & Swings, J. ( 2003; ). Reclassification of Vibrio hollisae as Grimontia hollisae gen. nov., comb. nov. Int J Syst Evol Microbiol 53, 1615–1617.[CrossRef]
    [Google Scholar]
  24. Thompson, F. L., Iida, T. & Swings, J. ( 2004; ). Biodiversity of vibrios. Microbiol Mol Biol Rev 68, 403–431.[CrossRef]
    [Google Scholar]
  25. Thompson, F. L., Gomez-Gil, B., Vasconcelos, A. T. R. & Sawabe, T. ( 2007; ). Multilocus sequence analysis reveals that Vibrio harveyi and V. campbellii are distinct species. Appl Environ Microbiol 73, 4279–4285.[CrossRef]
    [Google Scholar]
  26. Urbanczyk, H., Ast, J. C., Higgins, M. J., Carson, J. & Dunlap, P. V. ( 2007; ). Reclassification of Vibrio fischeri, Vibrio logei, Vibrio salmonicida and Vibrio wodanis as Aliivibrio fischeri gen. nov., comb. nov., Aliivibrio logei comb. nov., Aliivibrio salmonicida comb. nov. and Aliivibrio wodanis comb. nov. Int J Syst Evol Microbiol 57, 2823–2829.[CrossRef]
    [Google Scholar]
  27. Xie, C. H. & Yokota, A. ( 2003; ). Phylogenetic analyses of Lampropedia hyalina based on the 16S rRNA gene sequence. J Gen Appl Microbiol 49, 345–349.[CrossRef]
    [Google Scholar]
  28. Yang, Y., Yeh, L., Cao, Y., Baumann, L., Baumann, P., Tang, J. S. & Beaman, B. ( 1983; ). Characterization of marine luminous bacteria isolated off the coast of China and description of Vibrio orientalis sp. nov. Curr Microbiol 8, 95–100.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.004283-0
Loading
/content/journal/ijsem/10.1099/ijs.0.004283-0
Loading

Data & Media loading...

Supplements

[ Single PDF file] (66 KB)

PDF

[ Single PDF file] (2.5 MB)

PDF

Micrograph using atomic force microscopy of strain LC2-005 (=NBRC 104587 ) grown on solid medium. Bar, 2 µm.

IMAGE

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error