RT Journal Article SR Electronic(1) A1 Yoshizawa, Susumu A1 Wada, Minoru A1 Kita-Tsukamoto, Kumiko A1 Ikemoto, Eiko A1 Yokota, Akira A1 Kogure, KazuhiroYR 2009 T1 Vibrio azureus sp. nov., a luminous marine bacterium isolated from seawater JF International Journal of Systematic and Evolutionary Microbiology, VO 59 IS 7 SP 1645 OP 1649 DO https://doi.org/10.1099/ijs.0.004283-0 PB Microbiology Society, SN 1466-5034, AB Two luminous marine bacterial strains, LC2-005T and LC2-102, were isolated from seawater at Kuroshio Region and Sagami Bay in Japan, respectively. These bacteria were Gram-negative, oxidase-positive, catalase-positive, motile and rod-shaped. On the basis of 16S rRNA gene sequence analysis, strains LC2-005T and LC2-102 formed a cluster within the Vibrio harveyi species group. However, multilocus sequence analysis using five loci (pyrH, ftsZ, mreB, gyrB and gapA) and DNA–DNA hybridization experiments indicated that these strains were distinct from the currently known Vibrio species. Additionally, these strains differ from related Vibrio species in utilization of glucose, mannitol, inositol, sorbitol, rhamnose, sucrose, melibiose and arabinose, production of lysine decarboxylase, ornithine decarboxylase, tryptophan deaminase, esterase (C4), lipase (C4), chymotrypsin, acid phosphatase, α-glucosidase, β-glucosidase and N-acetyl-β-glucosaminidase and the ability to reduce nitrate to nitrite. The major fatty acids were C15 : 0 iso 2-OH and/or C16 : 1 ω7c, C16 : 0, C18 : 1 ω7c and C14 : 0. The DNA G+C contents of strains LC2-005T and LC2-102 were 45.2 and 45.5 mol%, respectively. On the basis of the polyphasic taxonomic evidence presented in this study, it can be concluded that strains LC2-005T and LC2-102 belong to the same genospecies and represent a novel species of the genus Vibrio, for which the name Vibrio azureus sp. nov. is proposed. The type strain is LC2-005T (=NBRC 104587T =KCTC 22352T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.004283-0