1887

Abstract

Spiroplasma strain CC-1, isolated from the gut of the soldier beetle , was serologically distinct from other spiroplasma species, groups, and subgroups. Cells of strain CC-1 were shown by light microscopy to be helical, motile filaments. Electron microscopy showed that the cells were bounded by a single cytoplasmic membrane, with no evidence of a cell wall. The organism was insensitive to penicillin. Strain CC-1 grew well in SM-1, M1D, and SP-4 liquid media under aerobic or anaerobic conditions. The strain also grew in 1% serum fraction medium. Optimal growth occurred at 32°C, with a doubling time of 2.6 h, but the strain multiplied at temperatures of 10 to 37°C. Strain CC-1 produced acid from glucose but hydrolyzed neither arginine nor urea. The guanine-plus-cytosine (G+C) content of the DNA was 26± 1 mol%. Other uncloned isolates from exhibited similar or identical serological patterns. On the basis of the data presented here, strain CC-1 (= ATCC 43207), previously proposed as the representative strain of subgroup XVI-1, is designated the type strain of a new species, .

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/content/journal/ijsem/10.1099/00207713-43-3-421
1993-07-01
2019-10-15
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http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/00207713-43-3-421
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