@article{mbs:/content/journal/ijsem/10.1099/00207713-43-3-421, author = "Whitcomb, Robert F. and Chastel, C. and Abalain-Colloc, M. and Stevens, C. and Tully, J. G. and Rose, D. L. and Carle, P. and Bové, J. M. and Henegar, R. B. and Hackett, K. J. and Clark, T. B. and Konai, M. and Williamson, D. L.", title = "Spiroplasma cantharicola sp. nov., from Cantharid Beetles (Coleoptera: Cantharidae)", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "1993", volume = "43", number = "3", pages = "421-424", doi = "https://doi.org/10.1099/00207713-43-3-421", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/00207713-43-3-421", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", abstract = "Abstract Spiroplasma strain CC-1T, isolated from the gut of the soldier beetle Cantharis carolinus, was serologically distinct from other spiroplasma species, groups, and subgroups. Cells of strain CC-1T were shown by light microscopy to be helical, motile filaments. Electron microscopy showed that the cells were bounded by a single cytoplasmic membrane, with no evidence of a cell wall. The organism was insensitive to penicillin. Strain CC-1T grew well in SM-1, MID, and SP-4 liquid media under aerobic or anaerobic conditions. The strain also grew in 1% serum fraction medium. Optimal growth occurred at 32°C, with a doubling time of 2.6 h, but the strain multiplied at temperatures of 10 to 37°C. Strain CC-1T produced acid from glucose but hydrolyzed neither arginine nor urea. The guanine-plus-cytosine (G+C) content of the DNA was 26 ± 1 mol%. Other uncloned isolates from C. carolinus exhibited similar or identical serological patterns. On the basis of the data presented here, strain CC-1T (= ATCC 43207), previously proposed as the representative strain of subgroup XVI-1, is designated the type strain of a new species, Spiroplasma cantharicola.", }