1887

Abstract

A molecular approach was used to examine the genetic relatedness of 19 isolates by measuring the extent of deoxyribonucleic acid-deoxyribonucleic acid homology and the fidelity of hybrid duplex molecules. The isolates examined were divided into two groups based on the results of hybridization tests. The members of genogroup 1, consisting of isolates from , and host plants, exhibited high levels of homology (67.4 to 94.1%) with strain ArI4, an isolate from . Isolates from , and and isolate AirI2 from did not exhibit significant homology (less than 39%) with strain ArI4. None of the strains showed a high degree of homology with strain EuI1a (less than 33%), an isolate from . Among the isolates not belonging to genogroup 1, subgroupings seemed to exist, as evidenced by a very high level of homology (97%) between two isolates from , strains D11 and the G2, but a low level of homology (27%) between other strains and strain G2. Thermal stability studies of the hybrid deoxyribonucleic acids which showed high levels of homology revealed an average mismatch of 3%, whereas the low-homology duplexes exhibited about 5% mismatch. The genome molecular weights of two probe strains, strains ArI4 and EuI1a, were 8.3 × 10and 6.0 × 10, respectively.

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/content/journal/ijsem/10.1099/00207713-35-2-140
1985-04-01
2019-12-07
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http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/00207713-35-2-140
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