1887

Abstract

We compared the protein and glycoprotein patterns of 39 strains of various origins by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The ultrastructures of the cell walls of intact cells were examined by freeze-etching, negative-staining, and thin-sectioning techniques. A total of 30 strains possessed a paracrystalline protein or glycoprotein surface layer (S-layer) which resembled the highest-molecular-weight band(s) on the electrophoretograms. We found a remarkable heterogeneity in the molecular weights of the S-layer subunits and the geometry and constants of the S-layer lattices, even among closely related strains. All of the strains examined were equally susceptible to lysozyme, indicating that the S-layers have pores at least 3.5 nm wide.

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/content/journal/ijsem/10.1099/00207713-34-2-202
1984-04-01
2019-10-22
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http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/00207713-34-2-202
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