RT Journal Article SR Electronic(1) A1 Messner, Paul A1 Hollaus, Friedrich A1 Sleytr, Uwe B.YR 1984 T1 Paracrystalline Cell Wall Surface Layers of Different Bacillus stearothermophilus Strains JF International Journal of Systematic and Evolutionary Microbiology, VO 34 IS 2 SP 202 OP 210 DO https://doi.org/10.1099/00207713-34-2-202 PB Microbiology Society, SN 1466-5034, AB We compared the protein and glycoprotein patterns of 39 Bacillus stearothermophilus strains of various origins by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The ultrastructures of the cell walls of intact cells were examined by freeze-etching, negative-staining, and thin-sectioning techniques. A total of 30 strains possessed a paracrystalline protein or glycoprotein surface layer (S-layer) which resembled the highest-molecular-weight band(s) on the electrophoretograms. We found a remarkable heterogeneity in the molecular weights of the S-layer subunits and the geometry and constants of the S-layer lattices, even among closely related strains. All of the strains examined were equally susceptible to lysozyme, indicating that the S-layers have pores at least 3.5 nm wide., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/00207713-34-2-202