1887

Abstract

Two-dimensional immunoelectrophoresis was used to study the antigenic composition and serological relationships of the following five species: , , , , and . A total of 15 to 25 precipitin peaks were observed in the homologous reactions. . and . were most closely related serologically, having six related antigens. Nearly all antigens were negatively charged at pH 8.6; the only exceptions were one antigen of . and one antigen of . . The “positively charged” antigens were membrane associated and did not cross-react. One common antigen was recognized in each organism by all heterologous antisera. Monospecific antiserum prepared against the common antigen (peak 0.72 of . ) reacted with one antigen in each species tested. The electrophoretic of the common antigen ranged from 0.45 (. ) to 0.72 (. ). This antigen was located in the cytoplasm of . because the antiserum reacted only with the cytoplasmic fractions, not with the membrane fractions. The recognition of a common cytoplasmic antigen in the is important to the taxonomy of these organisms.

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/content/journal/ijsem/10.1099/00207713-33-2-356
1983-04-01
2024-12-13
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