1887

Abstract

Relationships among selected species of viridans streptococci were studied by using deoxyribonucleic acid (DNA)-DNA hybridization and biochemical and serological tests. The results of hybridization experiments performed with DNA immobilized on membrane filters and index DNA made radioactive with [-H]thymidine indicated that ATCC 27335 (type strain), ATCC 27823, ATCC 9895, “ MG-intermedius” CDC SS 899, group F strain MGH 8153, and group F strain SFGH 879 are closely related to each other genetically, even though physiological tests indicated that this is a relatively heterogeneous group of organisms. The relative binding ratios for these six strains ranged from 60 to 99%, and the majority of values were greater than 85%, indicating that these strains should be considered members of the same species. Type strain ATCC 33399 (called ) is not a typical strain based on biochemical test results, and only moderate relatedness (41%) was observed in hybridization experiments performed with a typical strain (strain SS 429). I ATCC 10556 and II ATCC 10557 do not belong in the same species. A low relative binding ratio (13%) for these strains confirmed similar findings by previous investigators.

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1983-04-01
2022-08-10
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References

  1. Andrewes F. W., Horder T. J. 1906; A study of the streptococci pathogenic for man. Lancet 11:708–713 775-782, and 852-855
    [Google Scholar]
  2. Carlsson J. 1967; Presence of various types of nonhemolytic streptococci in dental plaque and in other sites of the oral cavity in man. Odontol. Revy 18:55–74
    [Google Scholar]
  3. Carlsson J. 1968; A numerical taxonomic study of human oral streptococci. Odontol. Revy 19:137–160
    [Google Scholar]
  4. Cole J. S. III, Kolstad R. A. 1974; Some atypical features of Streptococcus sanguis ATCC 10557. Int. J. Syst. Bacteriol. 24:370–372
    [Google Scholar]
  5. Cole R. M., Calandra G. B., Huff E., Nugent K. M. 1976; Attributes of potential utility in differentiating among “group H” streptococci or Streptococcus sanguis . J. Dent. Res. 55:A142–A153
    [Google Scholar]
  6. Colman G., Williams R. E. O. 1972; Taxonomy of some human viridans streptococci. 281–299 Wannamaker L. W., Matsen J. M. Streptococci and streptococcal diseases Academic Press, Inc.; New York:
    [Google Scholar]
  7. Coykendall A. L. 1970; Base composition of deoxyribonucleic acid isolated from cariogenic streptococci. Arch. Oral Biol. 15:365–368
    [Google Scholar]
  8. Coykendall A. L., Kaczmarek F. S., Slots J. 1980; Genetic heterogeneity in Bacteroides asaccharolyticus (Holdeman and Moore 1970) Finegold and Barnes 1977 (Approved Lists 1980) and proposal of Bacteroides gingivalis sp. nov. and Bacteroides macacae (Slots and Genco) comb. nov. Int. J. Syst. Bacteriol. 30:559–564
    [Google Scholar]
  9. Coykendall A. L., Specht P. A. 1975; DNA base sequence homologies among strains of Streptococcus sanguis . J. Gen. Microbiol. 91:92–98
    [Google Scholar]
  10. De Ley J., Tijtgat R. 1970; Evaluation of membrane filters for DNA-DNA hybridization. Antonie van Leeu-wenhoek. J. Microbiol. Serol. 36:461–474
    [Google Scholar]
  11. Dowell V. R. Jr., Hawkins T. M. 1977 Laboratory methods in anaerobic bacteriology Centers for Disease Control; Atlanta, Ga:
    [Google Scholar]
  12. Facklam R. R. 1977; Physiological differentiation of viridans streptococci. J. Clin. Microbiol. 5:184–201
    [Google Scholar]
  13. Guggenheim B. 1968; Streptococci of dental plaques. Caries Res. 2:147–163
    [Google Scholar]
  14. Guthof O. 1956; Pathogenic strains of Streptococcus viridans; streptococci found in dental abscesses and infiltrates in the region of the oral cavity. Zentralbl. Bakteriol. Naturwiss. 166:553–564
    [Google Scholar]
  15. Hardie J. M., Bowden G. H. 1976; Physiological classification of oral viridans streptococci. J. Dent. Res. 55:A166–A176
    [Google Scholar]
  16. Holdeman L. V., Moore W. E. C. 1974; New genus, Coprococcus twelve new species, and emended descriptions of four previously described species of bacteria from human feces. Int. J. Syst. Bacteriol. 24:260–277
    [Google Scholar]
  17. Horsfall F. L. Jr. 1951; Studies on non-hemolytic streptococci isolated from the respiratory tract of man. The antigenic basis for type specific reactions with Streptococcus salivarius and non-levan-forming streptococci. J. Exp. Med. 93:229–245
    [Google Scholar]
  18. Johnson J. L. 1981; Genetic characterization. 450–475 Gerhardt P. Manual of methods for general bacteriology American Society for Microbiology; Washington, D.C.:
    [Google Scholar]
  19. Jones D. 1978; Composition and differentiation of the genus Streptococcus . 1–40 Skinner F. A., Quensel L. B. Streptococci Academic Press, Inc.; New York:
    [Google Scholar]
  20. Lai C., Listgarten M., Rosan B. 1973; Serology of Streptococcus sanguis: localization of antigens with unlabeled antisera. Infect. Immun. 8:475–481
    [Google Scholar]
  21. Legault-Demare J., Desseaux B., Heyman T., Seror S., Ress G. P. 1967; Studies on hybrid molecules of nucleic acids. I. DNA-DNA hybrids on nitrocellulose filters. Biochem. Biophys. Res. Commun. 28:550–557
    [Google Scholar]
  22. Liu P. 1954; Carbon dioxide requirement of group F and minute colony G hemolytic streptococci. J. Bacteriol. 68:282–288
    [Google Scholar]
  23. Long P. H., Bliss E. A. 1934; Studies upon minute hemolytic streptococci. I. The isolation and cultural characteristics of minute betahemolytic streptococci. J. Exp. Med. 60:619–631
    [Google Scholar]
  24. Marmur J. 1961; A procedure for the isolation of deoxyri-bonucleic acid from microorganisms. J. Mol. Biol. 3:208–218
    [Google Scholar]
  25. Mirick G., Thomas L., Curnen E. C., Horsfall F. L. Jr. 1944; Studies on a non-hemolytic streptococcus isolated from the respiratory tract of human beings. J. Exp. Med. 80:391–440
    [Google Scholar]
  26. Ottens H., Winkler K. C. 1962; Indifferent and haemolytic streptococci possessing group-antigen. F. J. Gen. Microbiol. 28:181–191
    [Google Scholar]
  27. Parker M. T., Ball L. C. 1976; Streptococci and aerococci associated with systemic infection in man. J. Med. Microbiol. 9:275–302
    [Google Scholar]
  28. Poole P. M., Wilson G. 1976; Infection with minute-colonyforming beta-haemolytic streptococci. J. Clin. Pathol. 29:740–745
    [Google Scholar]
  29. Pulliam L., Forschen R. K., Hadley W. K. 1980; Biochemical properties of CO2-dependent streptococci. J. Clin. Microbiol. 12:27–31
    [Google Scholar]
  30. Rosan B. 1973; Antigens of Streptococcus sanguis . Infect. Immun. 7:205–211
    [Google Scholar]
  31. Ruoff K., Kunz L. J. 1982; Identification of viridans streptococci isolated from clinical specimens. J. Clin. Microbiol. 15:920–925
    [Google Scholar]
  32. Saito H., Miura K. 1963; Preparation of transforming DNA by phenol treatment. Biochim. Biophys. Acta 72:619–629
    [Google Scholar]
  33. Sherman J. M. 1937; The streptococci. Bacteriol. Rev. 1:3–97
    [Google Scholar]
  34. Skerman V. B. D., McGowan V., Sneath P. H. A. (ed.). 1980; Approved lists of bacterial names. Int. J. Syst. Bacteriol. 30:225–420
    [Google Scholar]
  35. Weissman S. M., Reich P. R., Somerson N. L., Cole R. M. 1966; Genetic differentiation by nucleic acid homology. IV. Relationships among Lancefield groups and serotypes of streptococci. J. Bacteriol. 92:1372–1377
    [Google Scholar]
  36. Williamson C. K. 1964; Serological classification of viridans streptococci from the respiratory tract of man. 607–622 Leone C. A. International conference on taxonomic biochemistry and serology The Ronald Press Co.; New York:
    [Google Scholar]
  37. Wilson G. S., Miles A. 1975; The streptococci. 712–763 In Principles of bacteriology, virology, and immunity 1 The Williams & Wilkins Co.; Baltimore:
    [Google Scholar]
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